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Determination of 4-methylpiperaine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rats′ plasma by online-SPE-HPLC-DAD: Application to a pharmacokinetic study
Zhang, Jianmei1; Lin, Wensi1; Li, Xiaona1; Yu, Ning1; Ling, Xiaomei1,2; Fu, Ge1; Li, Runtao2; Cui, Jingrong2
关键词Online-spe-hplc-dad Pharmacokinetic Study Tm208
刊名JOURNAL OF SEPARATION SCIENCE
2012-03-01
DOI10.1002/jssc.201100876
35期:5-6页:721-725
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]SOLID-PHASE EXTRACTION ; TANDEM MASS-SPECTROMETRY ; METABOLITES ; TM208 ; MS/MS ; LC
英文摘要

A specific, simple, and fast online-solid-phase extraction-high performance liquid chromatography-diode array detector (SPE-HPLC-DAD) method was developed and validated to quantify 4-methylpiperaine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride (TM208) in small volume samples of rats plasma for the first time. In this method, the 50-mu L plasma sample was taken to perform protein precipitation with 75 mu L methanol, and then 50 mu L supernatant containing the target analytes was injected and concentrated automatically in a C18 solid-phase extraction (SPE) cartridge. After that the sample was separated on a C18 RP analytical column and analyzed by DAD. The run cycle time is 6.0 min for each sample, and the calibration curve over the range of 0.03 to 25.00 mu g/mL has a good linear relationship (r > 0.9998). The recoveries of the quality control samples were all greater than 90%. The limit of detection and the lowest limit of quantification were 0.01 and 0.03 mu g/mL, respectively. Finally, this method was successfully applied to a pharmacokinetic study of TM208 in rats.

语种英语
WOS记录号WOS:000303040400015
项目编号81072612 ; 7102107 ; K20090207 ; 2009ZX09301-010
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; Open Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; National New Drug Research and Development Project
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51072
专题北京大学药学院_化学生物学系
北京大学药学院_分子与细胞药理学系
北京大学第一临床医学院_眼科
作者单位1.Peking Univ, Dept Pharmaceut Anal, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Jianmei,Lin, Wensi,Li, Xiaona,et al. Determination of 4-methylpiperaine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rats′ plasma by online-SPE-HPLC-DAD: Application to a pharmacokinetic study[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(5-6):721-725.
APA Zhang, Jianmei.,Lin, Wensi.,Li, Xiaona.,Yu, Ning.,Ling, Xiaomei.,...&Cui, Jingrong.(2012).Determination of 4-methylpiperaine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rats′ plasma by online-SPE-HPLC-DAD: Application to a pharmacokinetic study.JOURNAL OF SEPARATION SCIENCE,35(5-6),721-725.
MLA Zhang, Jianmei,et al."Determination of 4-methylpiperaine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rats′ plasma by online-SPE-HPLC-DAD: Application to a pharmacokinetic study".JOURNAL OF SEPARATION SCIENCE 35.5-6(2012):721-725.
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