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学科主题: 药学
题名:
Determination of 4-methylpiperaine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rats′ plasma by online-SPE-HPLC-DAD: Application to a pharmacokinetic study
作者: Zhang, Jianmei1; Lin, Wensi1; Li, Xiaona1; Yu, Ning1; Ling, Xiaomei1,2; Fu, Ge1; Li, Runtao2; Cui, Jingrong2
关键词: Online-SPE-HPLC-DAD ; Pharmacokinetic study ; TM208
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2012-03-01
DOI: 10.1002/jssc.201100876
卷: 35, 期:5-6, 页:721-725
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: SOLID-PHASE EXTRACTION ; TANDEM MASS-SPECTROMETRY ; METABOLITES ; TM208 ; MS/MS ; LC
英文摘要:

A specific, simple, and fast online-solid-phase extraction-high performance liquid chromatography-diode array detector (SPE-HPLC-DAD) method was developed and validated to quantify 4-methylpiperaine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride (TM208) in small volume samples of rats plasma for the first time. In this method, the 50-mu L plasma sample was taken to perform protein precipitation with 75 mu L methanol, and then 50 mu L supernatant containing the target analytes was injected and concentrated automatically in a C18 solid-phase extraction (SPE) cartridge. After that the sample was separated on a C18 RP analytical column and analyzed by DAD. The run cycle time is 6.0 min for each sample, and the calibration curve over the range of 0.03 to 25.00 mu g/mL has a good linear relationship (r > 0.9998). The recoveries of the quality control samples were all greater than 90%. The limit of detection and the lowest limit of quantification were 0.01 and 0.03 mu g/mL, respectively. Finally, this method was successfully applied to a pharmacokinetic study of TM208 in rats.

语种: 英语
所属项目编号: 81072612 ; 7102107 ; K20090207 ; 2009ZX09301-010
项目资助者: National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; Open Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; National New Drug Research and Development Project
WOS记录号: WOS:000303040400015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51072
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Dept Pharmaceut Anal, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Jianmei,Lin, Wensi,Li, Xiaona,et al. Determination of 4-methylpiperaine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rats′ plasma by online-SPE-HPLC-DAD: Application to a pharmacokinetic study[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(5-6):721-725.
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