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学科主题临床医学
Long-distance intercellular connectivity between cardiomyocytes and cardiofibroblasts mediated by membrane nanotubes
He, Kangmin1,2,3; Shi, Xiaoli1; Zhang, Xuejie1; Dang, Song1; Ma, Xiaowei2,3; Liu, Fei3; Xu, Ming2,3; Lv, Zhizhen2,3; Han, Dong4; Fang, Xiaohong1; Zhang, Youyi2,3
关键词Cardiomyocytes Cardiofibroblasts Membrane Nanotubes Mitochondria Calcium
刊名CARDIOVASCULAR RESEARCH
2011-10-01
DOI10.1093/cvr/cvr189
92期:1页:39-47
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems
资助者National Key Basic Research Program of People&prime ; s Republic of China ; Natural Science Foundation of China ; National Key Basic Research Program of People&prime ; s Republic of China ; Natural Science Foundation of China
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]TUNNELING NANOTUBES ; CARDIAC MYOCYTES ; ANIMAL-CELLS ; IN-VIVO ; FIBROBLASTS ; MACROPHAGES ; FACILITATE ; MECHANISM ; EXOCYST ; HEART
英文摘要

Aims Intercellular interactions between cardiomyocytes (CMs) and cardiofibroblasts (FBs) are important in the physiological and pathophysiological heart. Understanding such interactions is important for developing effective heart disease therapies. However, until recently, little has been known about these interactions. We aimed to investigate structural and functional connections between CMs and FBs that are distinct from gap junctions.

Methods and results By membrane dye staining, we observed long, thin membrane nanotubular structures containing actin and microtubules that connected neonatal rat ventricular CMs and FBs. By single-particle tracking, we observed vehicles moving between CMs and FBs within the membrane nanotubes. By dual colour staining, confocal imaging and flow cytometry, we observed mitochondria exchange between CMs and FBs in a coculture system. By combined atomic force microscopy (AFM) and confocal microscopy, we observed calcium signal propagation from AFM-stimulated CM (or FB) to unstimulated FB (or CM) via membrane nanotubes. By membrane and cytoskeleton staining, we observed similar nanotubular structures in adult mouse heart tissue, which suggests their physiological relevance.

Conclusions As a novel type of CM to FB communication, membrane nanotubes observed in vitro and in vivo provide structural and functional connectivity between CMs and FBs over long distances.

语种英语
所属项目编号2007CB935601 ; 81030001 ; 30821001 ; 81070196 ; 20821003
资助者National Key Basic Research Program of People&prime ; s Republic of China ; Natural Science Foundation of China ; National Key Basic Research Program of People&prime ; s Republic of China ; Natural Science Foundation of China
WOS记录号WOS:000294969000008
引用统计
被引频次:55[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51089
专题北京大学第三临床医学院_心血管内科
作者单位1.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Peking Univ, Inst Vasc Med, Hosp 3,Key Lab Cardiovasc Mol Biol & Regulatory P, Minist Educ,Key Lab Mol Cardiovasc Sci,Minist Hlt, Beijing 100191, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100191, Peoples R China
4.Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China
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GB/T 7714
He, Kangmin,Shi, Xiaoli,Zhang, Xuejie,et al. Long-distance intercellular connectivity between cardiomyocytes and cardiofibroblasts mediated by membrane nanotubes[J]. CARDIOVASCULAR RESEARCH,2011,92(1):39-47.
APA He, Kangmin.,Shi, Xiaoli.,Zhang, Xuejie.,Dang, Song.,Ma, Xiaowei.,...&Zhang, Youyi.(2011).Long-distance intercellular connectivity between cardiomyocytes and cardiofibroblasts mediated by membrane nanotubes.CARDIOVASCULAR RESEARCH,92(1),39-47.
MLA He, Kangmin,et al."Long-distance intercellular connectivity between cardiomyocytes and cardiofibroblasts mediated by membrane nanotubes".CARDIOVASCULAR RESEARCH 92.1(2011):39-47.
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