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学科主题: 临床医学
题名:
Long-distance intercellular connectivity between cardiomyocytes and cardiofibroblasts mediated by membrane nanotubes
作者: He, Kangmin1,2,3; Shi, Xiaoli1; Zhang, Xuejie1; Dang, Song1; Ma, Xiaowei2,3; Liu, Fei3; Xu, Ming2,3; Lv, Zhizhen2,3; Han, Dong4; Fang, Xiaohong1; Zhang, Youyi2,3
关键词: Cardiomyocytes ; Cardiofibroblasts ; Membrane nanotubes ; Mitochondria ; Calcium
刊名: CARDIOVASCULAR RESEARCH
发表日期: 2011-10-01
DOI: 10.1093/cvr/cvr189
卷: 92, 期:1, 页:39-47
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cardiac & Cardiovascular Systems
研究领域[WOS]: Cardiovascular System & Cardiology
关键词[WOS]: TUNNELING NANOTUBES ; CARDIAC MYOCYTES ; ANIMAL-CELLS ; IN-VIVO ; FIBROBLASTS ; MACROPHAGES ; FACILITATE ; MECHANISM ; EXOCYST ; HEART
英文摘要:

Aims Intercellular interactions between cardiomyocytes (CMs) and cardiofibroblasts (FBs) are important in the physiological and pathophysiological heart. Understanding such interactions is important for developing effective heart disease therapies. However, until recently, little has been known about these interactions. We aimed to investigate structural and functional connections between CMs and FBs that are distinct from gap junctions.

Methods and results By membrane dye staining, we observed long, thin membrane nanotubular structures containing actin and microtubules that connected neonatal rat ventricular CMs and FBs. By single-particle tracking, we observed vehicles moving between CMs and FBs within the membrane nanotubes. By dual colour staining, confocal imaging and flow cytometry, we observed mitochondria exchange between CMs and FBs in a coculture system. By combined atomic force microscopy (AFM) and confocal microscopy, we observed calcium signal propagation from AFM-stimulated CM (or FB) to unstimulated FB (or CM) via membrane nanotubes. By membrane and cytoskeleton staining, we observed similar nanotubular structures in adult mouse heart tissue, which suggests their physiological relevance.

Conclusions As a novel type of CM to FB communication, membrane nanotubes observed in vitro and in vivo provide structural and functional connectivity between CMs and FBs over long distances.

语种: 英语
所属项目编号: 2007CB935601 ; 81030001 ; 30821001 ; 81070196 ; 20821003
项目资助者: National Key Basic Research Program of People&prime ; s Republic of China ; Natural Science Foundation of China
WOS记录号: WOS:000294969000008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51089
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Peking Univ, Inst Vasc Med, Hosp 3,Key Lab Cardiovasc Mol Biol & Regulatory P, Minist Educ,Key Lab Mol Cardiovasc Sci,Minist Hlt, Beijing 100191, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100191, Peoples R China
4.Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China

Recommended Citation:
He, Kangmin,Shi, Xiaoli,Zhang, Xuejie,et al. Long-distance intercellular connectivity between cardiomyocytes and cardiofibroblasts mediated by membrane nanotubes[J]. CARDIOVASCULAR RESEARCH,2011,92(1):39-47.
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