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学科主题: 临床医学
题名:
A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer
作者: Hu, Yuntao1,2,6; Liu, Laura1,2,7; Lu, Bo4; Zhu, Danhong1,2,3; Ribeiro, Ramiro1,2,8; Diniz, Bruno1,2,9; Thomas, Padmaja B.1,2; Ahuja, Ashish K.1,2; Hinton, David R.1,2,3; Tai, Yu-Chong4; Hikita, Sherry T.5; Johnson, Lincoln V.5; Clegg, Dennis O.5; Thomas, Biju B.1,2; Humayun, Mark S.1,2
关键词: Transplantation ; Stem cells ; Parylene ; Retinal pigment epithelium ; Royal College of Surgeon rats
刊名: OPHTHALMIC RESEARCH
发表日期: 2012
DOI: 10.1159/000338749
卷: 48, 期:4, 页:186-191
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: BRUCHS MEMBRANE ; TRANSPLANTATION ; THERAPY ; DEGENERATION ; REGENERATION ; PROSPECTS ; DELIVERY
英文摘要:

Objective: To evaluate the feasibility of a new technique for the implantation of ultrathin substrates containing stem cell-derived retinal pigment epithelium (RPE) cells into the subretinal space of retina-degenerate Royal College of Surgeon (RCS) rats. Methods: A platform device was used for the implantation of 4-mu m-thick parylene substrates containing a monolayer of human embryonic stem cell-derived RPE (hESC-RPE). Normal Copenhagen rats (n = 6) and RCS rats (n = 5) were used for the study. Spectral-domain optical coherence tomography (SD-OCT) scanning and histological examinations were performed to confirm placement location of the implant. hESC- RPE cells attached to the substrate before and after implantation were evaluated using standard cell counting techniques. Results: SD-OCT scanning and histological examination revealed that the substrates were precisely placed in the rat′s subretinal space. The hESC-RPE cell monolayer that covered the surface of the substrate was found to be intact after implantation. Cell counting data showed that less than 2% of cells were lost from the substrate due to the implantation procedure (preimplantation count 2,792 +/- 74.09 cells versus postimplantation count 2,741 +/- 62.08 cells). Detailed microscopic examination suggested that the cell loss occurred mostly along the edges of the implant. Conclusion: With the help of this platform device, it is possible to implant ultrathin substrates containing an RPE monolayer into the rat′s subretinal space. This technique can be a useful approach for stem cell-based tissue bioengineering techniques in retinal transplantation research. Copyright (c) 2012 S. Karger AG, Basel

语种: 英语
所属项目编号: DRI-01444 ; EY03040
项目资助者: CIRM ; Research to Prevent Blindness and NEI
WOS记录号: WOS:000307874500004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51093
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: 1.Univ So Calif, Doheny Eye Inst, Keck Sch Med, Los Angeles, CA 90033 USA
2.Univ So Calif, Dept Ophthalmol, Keck Sch Med, Los Angeles, CA 90033 USA
3.Univ So Calif, Keck Sch Med, Dept Pathol, Los Angeles, CA 90033 USA
4.CALTECH, Pasadena, CA 91125 USA
5.Univ Calif Santa Barbara, Ctr Stem Cell Biol & Engn, Santa Barbara, CA 93106 USA
6.Chang Gung Mem Hosp, Dept Ophthalmol, Linkou, Taiwan
7.Hosp Evangel Curitiba, Curitiba, Parana, Brazil
8.Univ Fed Sao Paulo, Retina Sect, Sao Paulo, Brazil
9.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China

Recommended Citation:
Hu, Yuntao,Liu, Laura,Lu, Bo,et al. A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer[J]. OPHTHALMIC RESEARCH,2012,48(4):186-191.
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