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学科主题临床医学
A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer
Hu, Yuntao1,2,6; Liu, Laura1,2,7; Lu, Bo4; Zhu, Danhong1,2,3; Ribeiro, Ramiro1,2,8; Diniz, Bruno1,2,9; Thomas, Padmaja B.1,2; Ahuja, Ashish K.1,2; Hinton, David R.1,2,3; Tai, Yu-Chong4; Hikita, Sherry T.5; Johnson, Lincoln V.5; Clegg, Dennis O.5; Thomas, Biju B.1,2; Humayun, Mark S.1,2
关键词Transplantation Stem Cells Parylene Retinal Pigment Epithelium Royal College Of Surgeon Rats
刊名OPHTHALMIC RESEARCH
2012
DOI10.1159/000338749
48期:4页:186-191
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
资助者CIRM ; Research to Prevent Blindness and NEI ; CIRM ; Research to Prevent Blindness and NEI
研究领域[WOS]Ophthalmology
关键词[WOS]BRUCHS MEMBRANE ; TRANSPLANTATION ; THERAPY ; DEGENERATION ; REGENERATION ; PROSPECTS ; DELIVERY
英文摘要

Objective: To evaluate the feasibility of a new technique for the implantation of ultrathin substrates containing stem cell-derived retinal pigment epithelium (RPE) cells into the subretinal space of retina-degenerate Royal College of Surgeon (RCS) rats. Methods: A platform device was used for the implantation of 4-mu m-thick parylene substrates containing a monolayer of human embryonic stem cell-derived RPE (hESC-RPE). Normal Copenhagen rats (n = 6) and RCS rats (n = 5) were used for the study. Spectral-domain optical coherence tomography (SD-OCT) scanning and histological examinations were performed to confirm placement location of the implant. hESC- RPE cells attached to the substrate before and after implantation were evaluated using standard cell counting techniques. Results: SD-OCT scanning and histological examination revealed that the substrates were precisely placed in the rat′s subretinal space. The hESC-RPE cell monolayer that covered the surface of the substrate was found to be intact after implantation. Cell counting data showed that less than 2% of cells were lost from the substrate due to the implantation procedure (preimplantation count 2,792 +/- 74.09 cells versus postimplantation count 2,741 +/- 62.08 cells). Detailed microscopic examination suggested that the cell loss occurred mostly along the edges of the implant. Conclusion: With the help of this platform device, it is possible to implant ultrathin substrates containing an RPE monolayer into the rat′s subretinal space. This technique can be a useful approach for stem cell-based tissue bioengineering techniques in retinal transplantation research. Copyright (c) 2012 S. Karger AG, Basel

语种英语
所属项目编号DRI-01444 ; EY03040
资助者CIRM ; Research to Prevent Blindness and NEI ; CIRM ; Research to Prevent Blindness and NEI
WOS记录号WOS:000307874500004
Citation statistics
Cited Times:59[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51093
Collection北京大学第三临床医学院_眼科
作者单位1.Univ So Calif, Doheny Eye Inst, Keck Sch Med, Los Angeles, CA 90033 USA
2.Univ So Calif, Dept Ophthalmol, Keck Sch Med, Los Angeles, CA 90033 USA
3.Univ So Calif, Keck Sch Med, Dept Pathol, Los Angeles, CA 90033 USA
4.CALTECH, Pasadena, CA 91125 USA
5.Univ Calif Santa Barbara, Ctr Stem Cell Biol & Engn, Santa Barbara, CA 93106 USA
6.Chang Gung Mem Hosp, Dept Ophthalmol, Linkou, Taiwan
7.Hosp Evangel Curitiba, Curitiba, Parana, Brazil
8.Univ Fed Sao Paulo, Retina Sect, Sao Paulo, Brazil
9.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Hu, Yuntao,Liu, Laura,Lu, Bo,et al. A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer[J]. OPHTHALMIC RESEARCH,2012,48(4):186-191.
APA Hu, Yuntao.,Liu, Laura.,Lu, Bo.,Zhu, Danhong.,Ribeiro, Ramiro.,...&Humayun, Mark S..(2012).A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer.OPHTHALMIC RESEARCH,48(4),186-191.
MLA Hu, Yuntao,et al."A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer".OPHTHALMIC RESEARCH 48.4(2012):186-191.
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