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学科主题临床医学
In Vivo Confocal Microscopic Observation of Lamellar Corneal Transplantation in the Rabbit Using Xenogenic Acellular Corneal Scaffolds as a Substitute
Feng, Yun; Wang, Wei
关键词Corneal Substitute Decellularization Lamellar Corneal Transplantation Xenogenic Acellular Corneal Scaffolds
刊名CHINESE MEDICAL JOURNAL
2015-04-05
DOI10.4103/0366-6999.154301
128期:7页:933-940
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]CD4(+) T-CELLS ; PORCINE CORNEA ; MATRIX ; REJECTION ; RECONSTRUCTION ; COLLAGEN ; MICE ; WEAK
英文摘要

Background: The limiting factor to corneal transplantation is the availability of donors. Research has suggested that xenogenic acellular corneal scaffolds (XACS) may be a possible alternative to transplantation. This study aimed to investigate the viability of performing lamellar corneal transplantation (LCT) in rabbits using canine XACS.

Methods: Fresh dog corneas were decellularized by serial digestion, and LCT was performed on rabbit eyes using xenogeneic decellularized corneal matrix. Cellular and morphological changes were observed by slit-lamp, light, and scanning electron microscopy at 7, 30 and 90 days postoperatively. Immunocytochemical staining for specific markers such as keratin 3, vimentin and MUC5AC, was used to identify cells in the graft.

Results: Decellularized xenogenic corneal matrix remained transparent for about 1-month after LCT. The recipient cells were able to survive and proliferate into the grafts. Three months after transplantation, grafts had merged with host tissue, and graft epithelialization and vascularization had occurred. Corneal nerve fibers were able to grow into the graft in rabbits transplanted with XACS.

Conclusions: Xenogenic acellular corneal scaffolds can maintain the transparency of corneal grafts about 1-month and permit growth of cells and nerve fibers, and is, therefore, a potential substitute or carrier for a replacement cornea.

语种英语
WOS记录号WOS:000352533500014
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51098
专题北京大学第三临床医学院_眼科
作者单位Peking Univ, Hosp 3, Ctr Eye, Dept Ophthalmol, Beijing 100191, Peoples R China
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Feng, Yun,Wang, Wei. In Vivo Confocal Microscopic Observation of Lamellar Corneal Transplantation in the Rabbit Using Xenogenic Acellular Corneal Scaffolds as a Substitute[J]. CHINESE MEDICAL JOURNAL,2015,128(7):933-940.
APA Feng, Yun,&Wang, Wei.(2015).In Vivo Confocal Microscopic Observation of Lamellar Corneal Transplantation in the Rabbit Using Xenogenic Acellular Corneal Scaffolds as a Substitute.CHINESE MEDICAL JOURNAL,128(7),933-940.
MLA Feng, Yun,et al."In Vivo Confocal Microscopic Observation of Lamellar Corneal Transplantation in the Rabbit Using Xenogenic Acellular Corneal Scaffolds as a Substitute".CHINESE MEDICAL JOURNAL 128.7(2015):933-940.
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