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学科主题: 临床医学
题名:
In Vivo Confocal Microscopic Observation of Lamellar Corneal Transplantation in the Rabbit Using Xenogenic Acellular Corneal Scaffolds as a Substitute
作者: Feng, Yun; Wang, Wei
关键词: Corneal Substitute ; Decellularization ; Lamellar Corneal Transplantation ; Xenogenic Acellular Corneal Scaffolds
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2015-04-05
DOI: 10.4103/0366-6999.154301
卷: 128, 期:7, 页:933-940
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: CD4(+) T-CELLS ; PORCINE CORNEA ; MATRIX ; REJECTION ; RECONSTRUCTION ; COLLAGEN ; MICE ; WEAK
英文摘要:

Background: The limiting factor to corneal transplantation is the availability of donors. Research has suggested that xenogenic acellular corneal scaffolds (XACS) may be a possible alternative to transplantation. This study aimed to investigate the viability of performing lamellar corneal transplantation (LCT) in rabbits using canine XACS.

Methods: Fresh dog corneas were decellularized by serial digestion, and LCT was performed on rabbit eyes using xenogeneic decellularized corneal matrix. Cellular and morphological changes were observed by slit-lamp, light, and scanning electron microscopy at 7, 30 and 90 days postoperatively. Immunocytochemical staining for specific markers such as keratin 3, vimentin and MUC5AC, was used to identify cells in the graft.

Results: Decellularized xenogenic corneal matrix remained transparent for about 1-month after LCT. The recipient cells were able to survive and proliferate into the grafts. Three months after transplantation, grafts had merged with host tissue, and graft epithelialization and vascularization had occurred. Corneal nerve fibers were able to grow into the graft in rabbits transplanted with XACS.

Conclusions: Xenogenic acellular corneal scaffolds can maintain the transparency of corneal grafts about 1-month and permit growth of cells and nerve fibers, and is, therefore, a potential substitute or carrier for a replacement cornea.

语种: 英语
所属项目编号: SKW 06-9-06 ; 77470-01 ; 77470-02
项目资助者: Beijing Municipal Science and Technology Commission ; Peking university 3rd hospital ; Ph.D. Programs Foundation of Ministry of Education of China
WOS记录号: WOS:000352533500014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51098
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: Peking Univ, Hosp 3, Ctr Eye, Dept Ophthalmol, Beijing 100191, Peoples R China

Recommended Citation:
Feng, Yun,Wang, Wei. In Vivo Confocal Microscopic Observation of Lamellar Corneal Transplantation in the Rabbit Using Xenogenic Acellular Corneal Scaffolds as a Substitute[J]. CHINESE MEDICAL JOURNAL,2015,128(7):933-940.
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