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学科主题: 药学
题名:
A Novel Fluorescent Cell Membrane-permeable Caged Cyclic ADP-ribose Analogue
作者: Yu, Pei-Lin1,2; Zhang, Zhe-Hao2; Hao, Bai-Xia2; Zhao, Yong-Juan2; Zhang, Li-He1; Lee, Hon-Cheung2; Zhang, Liangren1; Yue, Jianbo2
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2012-07-13
DOI: 10.1074/jbc.M111.329854
卷: 287, 期:29, 页:24774-24783
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: INOSINE DIPHOSPHORIBOSE ; RYANODINE RECEPTOR ; CALCIUM-RELEASE ; CA2+ INFLUX ; CADPR ; ACTIVATION ; CHANNEL ; TRPM2 ; DERIVATIVES ; DISSOCIATION
英文摘要:

Cyclic adenosine diphosphate ribose is an endogenous Ca2+ mobilizer involved in diverse cellular processes. A cell membrane-permeable cyclic adenosine diphosphate ribose analogue, cyclic inosine diphosphoribose ether (cIDPRE), can induce Ca2+ increase in intact human Jurkat T-lymphocytes. Here we synthesized a coumarin-caged analogue of cIDPRE (Co-i-cIDPRE), aiming to have a precisely temporal and spatial control of bioactive cIDPRE release inside the cell using UV uncaging. We showed that Co-i-cIDPRE accumulated inside Jurkat cells quickly and efficiently. Uncaging of Co-i-cIDPRE evoked Ca2+ release from endoplasmic reticulum, with concomitant Ca2+ influx in Jurkat cells. Ca2+ release evoked by uncaged Co-i-cIDPRE was blocked by knockdown of ryanodine receptors (RyRs) 2 and 3 in Jurkat cells. The associated Ca2+ influx, on the other hand, was abolished by double knockdown of Stim1 and TRPM2 in Jurkat cells. Furthermore, Ca2+ release or influx evoked by uncaged Co-i-cIDPRE was recapitulated in HEK293 cells that overexpress RyRs or TRPM2, respectively, but not in wild-type cells lacking these channels. In summary, our results indicate that uncaging of Co-i-cIDPRE incites Ca2+ release from endoplasmic reticulum via RyRs and triggers Ca2+ influx via TRPM2.

语种: 英语
所属项目编号: HKU 784710M ; HKU 782709M ; HKU 785911M ; N_HKU 737/09 ; 20910094 ; 90713005
项目资助者: Research Grant Council (RGC) ; National Natural Science Foundation of China (NSFC)/RGC grant from Hong Kong ; NSFC ; Leukemia and Lymphoma Society of America
WOS记录号: WOS:000306651100070
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51106
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Univ Hong Kong, Dept Physiol, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Yu, Pei-Lin,Zhang, Zhe-Hao,Hao, Bai-Xia,et al. A Novel Fluorescent Cell Membrane-permeable Caged Cyclic ADP-ribose Analogue[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(29):24774-24783.
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