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A Novel Fluorescent Cell Membrane-permeable Caged Cyclic ADP-ribose Analogue
Yu, Pei-Lin1,2; Zhang, Zhe-Hao2; Hao, Bai-Xia2; Zhao, Yong-Juan2; Zhang, Li-He1; Lee, Hon-Cheung2; Zhang, Liangren1; Yue, Jianbo2
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2012-07-13
DOI10.1074/jbc.M111.329854
287期:29页:24774-24783
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]INOSINE DIPHOSPHORIBOSE ; RYANODINE RECEPTOR ; CALCIUM-RELEASE ; CA2+ INFLUX ; CADPR ; ACTIVATION ; CHANNEL ; TRPM2 ; DERIVATIVES ; DISSOCIATION
英文摘要

Cyclic adenosine diphosphate ribose is an endogenous Ca2+ mobilizer involved in diverse cellular processes. A cell membrane-permeable cyclic adenosine diphosphate ribose analogue, cyclic inosine diphosphoribose ether (cIDPRE), can induce Ca2+ increase in intact human Jurkat T-lymphocytes. Here we synthesized a coumarin-caged analogue of cIDPRE (Co-i-cIDPRE), aiming to have a precisely temporal and spatial control of bioactive cIDPRE release inside the cell using UV uncaging. We showed that Co-i-cIDPRE accumulated inside Jurkat cells quickly and efficiently. Uncaging of Co-i-cIDPRE evoked Ca2+ release from endoplasmic reticulum, with concomitant Ca2+ influx in Jurkat cells. Ca2+ release evoked by uncaged Co-i-cIDPRE was blocked by knockdown of ryanodine receptors (RyRs) 2 and 3 in Jurkat cells. The associated Ca2+ influx, on the other hand, was abolished by double knockdown of Stim1 and TRPM2 in Jurkat cells. Furthermore, Ca2+ release or influx evoked by uncaged Co-i-cIDPRE was recapitulated in HEK293 cells that overexpress RyRs or TRPM2, respectively, but not in wild-type cells lacking these channels. In summary, our results indicate that uncaging of Co-i-cIDPRE incites Ca2+ release from endoplasmic reticulum via RyRs and triggers Ca2+ influx via TRPM2.

语种英语
WOS记录号WOS:000306651100070
项目编号HKU 784710M ; HKU 782709M ; HKU 785911M ; N_HKU 737/09 ; 20910094 ; 90713005
资助机构Research Grant Council (RGC) ; National Natural Science Foundation of China (NSFC)/RGC grant from Hong Kong ; NSFC ; Leukemia and Lymphoma Society of America
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51106
专题北京大学药学院
北京大学药学院_药物化学系
作者单位1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Univ Hong Kong, Dept Physiol, Hong Kong, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Yu, Pei-Lin,Zhang, Zhe-Hao,Hao, Bai-Xia,et al. A Novel Fluorescent Cell Membrane-permeable Caged Cyclic ADP-ribose Analogue[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(29):24774-24783.
APA Yu, Pei-Lin.,Zhang, Zhe-Hao.,Hao, Bai-Xia.,Zhao, Yong-Juan.,Zhang, Li-He.,...&Yue, Jianbo.(2012).A Novel Fluorescent Cell Membrane-permeable Caged Cyclic ADP-ribose Analogue.JOURNAL OF BIOLOGICAL CHEMISTRY,287(29),24774-24783.
MLA Yu, Pei-Lin,et al."A Novel Fluorescent Cell Membrane-permeable Caged Cyclic ADP-ribose Analogue".JOURNAL OF BIOLOGICAL CHEMISTRY 287.29(2012):24774-24783.
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