北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 麻醉科  > 期刊论文
学科主题: 临床医学
题名:
In Vitro Kinetic Evaluation of the Free Radical Scavenging Ability of Propofol
作者: Li, Weiguang1; Zhang, Yu2; Liu, Yanru1; Yue, Feng3; Lu, Yiming1; Qiu, Huanrong2; Gao, Dawen1; Gao, Yan1; Wu, Yonghong1; Wang, Zhaoyan4; Huang, Rongqing1; Zhang, Chenggang1
刊名: ANESTHESIOLOGY
发表日期: 2012-06-01
DOI: 10.1097/ALN.0b013e3182567dcc
卷: 116, 期:6, 页:1258-1266
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Anesthesiology
研究领域[WOS]: Anesthesiology
关键词[WOS]: CELL ANTIOXIDANT CAPACITY ; ANESTHETIC AGENT PROPOFOL ; ELECTRON-SPIN-RESONANCE ; ALPHA-TOCOPHEROL ; RED-CELL ; CEREBRAL-ISCHEMIA ; SINGLET OXYGEN ; POLYPHENOLS ; PRODUCTS ; PEROXYNITRITE
英文摘要:

Background: Propofol is a widely used, short-acting, and intravenously administered hypnotic agent with notable antioxidant and free radical scavenging activities. However, there are relatively few kinetic studies on the free radical scavenging ability of propofol. The goal of this study is to evaluate the kinetics of propofol scavenging 2,2′-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) (ABTS) radical (ABTS(.+)).

Methods: The stock solution of ABTS(.+) was prepared by incubating 7 mM ABTS with 2.8 mM potassium persulfate in deionized water, and then diluted with 5 mM phosphate-buffered saline (pH 7.2) to get a working solution (36 mu M ABTS(.+) and 18 mu M ABTS). The reaction was monitored by measuring specific absorbance changes of ABTS and ABTS(.+) after adding 4 mu M propofol (final concentration) to the working solution. The propofol-ABTS(.+) reaction products were analyzed by high-performance liquid chromatography and liquid chromatography mass spectrometry/mass spectrometry.

Results: Wave scanning and kinetic evaluation demonstrated that the ABTS(.+) scavenging process of propofol is relatively fast. The ABTS(.+) consumption rate by propofol is greater than the rate of ABTS formation. The degradation products of reaction between propofol and ABTS(.+) were mainly ABTS-propofol, a part of the ABTS molecule, and a combination of propofol with a part of the ABTS molecule.

Conclusions: Propofol scavenges ABTS(.+) with a fast and stable kinetic feature in vitro, which is useful and important for understanding propofol′s antioxidant properties. The kinetic process of the free radical scavenging activity of propofol may also play a role in dynamic protection in the body.

语种: 英语
所属项目编号: 2012CB518200 ; 2012ZX09102301-016 ; 81100979 ; 81070741 ; 30973107 ; SKLP-K201004
项目资助者: National Basic Research Project (973 program) ; Ministry of Science and Technology of the People&prime ; s Republic of China (MOST), Beijing, China ; Special Key Program for Science and Technology of China ; Natural Science Foundation of China by National Natural Science Foundation of China (NSFC), Beijing, China ; State Key Laboratory of Proteomics, Beijing, China
WOS记录号: WOS:000304356500016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51117
Appears in Collections:北京大学第三临床医学院_麻醉科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Beijing Inst Radiat Med, State Key Lab Prote, Cognit & Mental Hlth Res Ctr, Beijing 100850, Peoples R China
2.Beijing Union Med Coll Hosp, Dept Anesthesiol, Beijing, Peoples R China
3.Peking Univ Third Hosp, Dept Anesthesiol, Beijing, Peoples R China
4.Gen Hosp Chinese PLA, Dept Ophthalmol, Beijing, Peoples R China

Recommended Citation:
Li, Weiguang,Zhang, Yu,Liu, Yanru,et al. In Vitro Kinetic Evaluation of the Free Radical Scavenging Ability of Propofol[J]. ANESTHESIOLOGY,2012,116(6):1258-1266.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Weiguang]'s Articles
[Zhang, Yu]'s Articles
[Liu, Yanru]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Weiguang]‘s Articles
[Zhang, Yu]‘s Articles
[Liu, Yanru]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace