IR@PKUHSC  > 北京大学第三临床医学院  > 麻醉科
学科主题临床医学
In Vitro Kinetic Evaluation of the Free Radical Scavenging Ability of Propofol
Li, Weiguang1; Zhang, Yu2; Liu, Yanru1; Yue, Feng3; Lu, Yiming1; Qiu, Huanrong2; Gao, Dawen1; Gao, Yan1; Wu, Yonghong1; Wang, Zhaoyan4; Huang, Rongqing1; Zhang, Chenggang1
刊名ANESTHESIOLOGY
2012-06-01
DOI10.1097/ALN.0b013e3182567dcc
116期:6页:1258-1266
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Anesthesiology
资助者National Basic Research Project (973 program) ; Ministry of Science and Technology of the People&prime ; s Republic of China (MOST), Beijing, China ; Special Key Program for Science and Technology of China ; Natural Science Foundation of China by National Natural Science Foundation of China (NSFC), Beijing, China ; State Key Laboratory of Proteomics, Beijing, China ; National Basic Research Project (973 program) ; Ministry of Science and Technology of the People&prime ; s Republic of China (MOST), Beijing, China ; Special Key Program for Science and Technology of China ; Natural Science Foundation of China by National Natural Science Foundation of China (NSFC), Beijing, China ; State Key Laboratory of Proteomics, Beijing, China
研究领域[WOS]Anesthesiology
关键词[WOS]CELL ANTIOXIDANT CAPACITY ; ANESTHETIC AGENT PROPOFOL ; ELECTRON-SPIN-RESONANCE ; ALPHA-TOCOPHEROL ; RED-CELL ; CEREBRAL-ISCHEMIA ; SINGLET OXYGEN ; POLYPHENOLS ; PRODUCTS ; PEROXYNITRITE
英文摘要

Background: Propofol is a widely used, short-acting, and intravenously administered hypnotic agent with notable antioxidant and free radical scavenging activities. However, there are relatively few kinetic studies on the free radical scavenging ability of propofol. The goal of this study is to evaluate the kinetics of propofol scavenging 2,2′-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) (ABTS) radical (ABTS(.+)).

Methods: The stock solution of ABTS(.+) was prepared by incubating 7 mM ABTS with 2.8 mM potassium persulfate in deionized water, and then diluted with 5 mM phosphate-buffered saline (pH 7.2) to get a working solution (36 mu M ABTS(.+) and 18 mu M ABTS). The reaction was monitored by measuring specific absorbance changes of ABTS and ABTS(.+) after adding 4 mu M propofol (final concentration) to the working solution. The propofol-ABTS(.+) reaction products were analyzed by high-performance liquid chromatography and liquid chromatography mass spectrometry/mass spectrometry.

Results: Wave scanning and kinetic evaluation demonstrated that the ABTS(.+) scavenging process of propofol is relatively fast. The ABTS(.+) consumption rate by propofol is greater than the rate of ABTS formation. The degradation products of reaction between propofol and ABTS(.+) were mainly ABTS-propofol, a part of the ABTS molecule, and a combination of propofol with a part of the ABTS molecule.

Conclusions: Propofol scavenges ABTS(.+) with a fast and stable kinetic feature in vitro, which is useful and important for understanding propofol′s antioxidant properties. The kinetic process of the free radical scavenging activity of propofol may also play a role in dynamic protection in the body.

语种英语
所属项目编号2012CB518200 ; 2012ZX09102301-016 ; 81100979 ; 81070741 ; 30973107 ; SKLP-K201004
资助者National Basic Research Project (973 program) ; Ministry of Science and Technology of the People&prime ; s Republic of China (MOST), Beijing, China ; Special Key Program for Science and Technology of China ; Natural Science Foundation of China by National Natural Science Foundation of China (NSFC), Beijing, China ; State Key Laboratory of Proteomics, Beijing, China ; National Basic Research Project (973 program) ; Ministry of Science and Technology of the People&prime ; s Republic of China (MOST), Beijing, China ; Special Key Program for Science and Technology of China ; Natural Science Foundation of China by National Natural Science Foundation of China (NSFC), Beijing, China ; State Key Laboratory of Proteomics, Beijing, China
WOS记录号WOS:000304356500016
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51117
Collection北京大学第三临床医学院_麻醉科
作者单位1.Beijing Inst Radiat Med, State Key Lab Prote, Cognit & Mental Hlth Res Ctr, Beijing 100850, Peoples R China
2.Beijing Union Med Coll Hosp, Dept Anesthesiol, Beijing, Peoples R China
3.Peking Univ Third Hosp, Dept Anesthesiol, Beijing, Peoples R China
4.Gen Hosp Chinese PLA, Dept Ophthalmol, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Li, Weiguang,Zhang, Yu,Liu, Yanru,et al. In Vitro Kinetic Evaluation of the Free Radical Scavenging Ability of Propofol[J]. ANESTHESIOLOGY,2012,116(6):1258-1266.
APA Li, Weiguang.,Zhang, Yu.,Liu, Yanru.,Yue, Feng.,Lu, Yiming.,...&Zhang, Chenggang.(2012).In Vitro Kinetic Evaluation of the Free Radical Scavenging Ability of Propofol.ANESTHESIOLOGY,116(6),1258-1266.
MLA Li, Weiguang,et al."In Vitro Kinetic Evaluation of the Free Radical Scavenging Ability of Propofol".ANESTHESIOLOGY 116.6(2012):1258-1266.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Li, Weiguang]'s Articles
[Zhang, Yu]'s Articles
[Liu, Yanru]'s Articles
百度学术
百度学术Similar articles in
[Li, Weiguang]'s Articles
[Zhang, Yu]'s Articles
[Liu, Yanru]'s Articles
必应学术
必应学术Similar articles in
[Li, Weiguang]'s Articles
[Zhang, Yu]'s Articles
[Liu, Yanru]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.