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学科主题临床医学
Apelin supports primary rat retinal Muller cells under chemical hypoxia and glucose deprivation
Wang, Xin-Lei1; Tao, Yong1; Lu, Qiang1; Jiang, Yan-Rong1
关键词Apelin Apj Muller Cells Glucose Deprivaion Hypoxia Ischemia
刊名PEPTIDES
2012-02-01
DOI10.1016/j.peptides.2011.12.015
33期:2页:298-306
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
资助者Beijing Novel Program ; EFSD/CDS/Lilly ; research fund for the Doctoral Program of Higher Education of China ; Science and Technology Project of Beijing ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; People&prime ; s Hospital of Peking University ; Beijing Novel Program ; EFSD/CDS/Lilly ; research fund for the Doctoral Program of Higher Education of China ; Science and Technology Project of Beijing ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; People&prime ; s Hospital of Peking University
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]ELEVATION MYOCARDIAL-INFARCTION ; LIGAND APELIN ; PROLIFERATION ; EXPRESSION ; APOPTOSIS ; PROTECTS ; VESSELS ; BARRIER ; GROWTH ; EDEMA
英文摘要

Muller cells support the integrity of the blood-retinal barrier, whereas their dysfunction under pathological conditions may contribute to retinal edema formation. The apelin peptide, as the endogenous ligand of G protein-coupled receptor APJ, participates in numbers of physiological and pathological processes. Recent studies highlight its emerging role against ischemic injury. Our study aimed to investigate the potential neuroprotection of apelin for primary rat retinal Muller cells under hypoxia or glucose-deprivation (GD) by cell viability, migration and apoptosis, as well as apelin/APJ immunofluorescence labeling and mRNA expression. The results showed that exogenous apelin significantly stimulated Muller cells viability and migration under normal, hypoxic and glucose-free condition, also prevented apoptosis. Apelin immunoreactivities represented weak and diffuse staining in the cytoplasm, along with restricted nuclear APJ expression. They both appeared stronger immunoreactivities after 12 h hypoxia. Under hypoxic stress, apelin mRNA expression began to increase at 6 h (9.97 folds, p < 0.01), and APJ mRNA also up-regulated (2 h 6.50 folds, p < 0.05; 4 h 2.25 folds, p < 0.05; 6 h 14 folds, p < 0.01), whereas they both down-regulated during 4-12 h GD. Our results suggested that apelin induced the tolerance of Muller cells to hypoxia and GD. Its administration might be a promising protection for blood-retinal barrier to ischemia. (C) 2012 Elsevier Inc. All rights reserved.

语种英语
所属项目编号2009B04 ; 90561 ; 20100001110073 ; Z08000303220801 ; 7112142 ; 30901639 ; 81041005 ; RDB2009-42
资助者Beijing Novel Program ; EFSD/CDS/Lilly ; research fund for the Doctoral Program of Higher Education of China ; Science and Technology Project of Beijing ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; People&prime ; s Hospital of Peking University ; Beijing Novel Program ; EFSD/CDS/Lilly ; research fund for the Doctoral Program of Higher Education of China ; Science and Technology Project of Beijing ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; People&prime ; s Hospital of Peking University
WOS记录号WOS:000301871200016
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51125
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Minist Educ, Key Lab Vis Loss & Restorat, Beijing 100044, Peoples R China
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GB/T 7714
Wang, Xin-Lei,Tao, Yong,Lu, Qiang,et al. Apelin supports primary rat retinal Muller cells under chemical hypoxia and glucose deprivation[J]. PEPTIDES,2012,33(2):298-306.
APA Wang, Xin-Lei,Tao, Yong,Lu, Qiang,&Jiang, Yan-Rong.(2012).Apelin supports primary rat retinal Muller cells under chemical hypoxia and glucose deprivation.PEPTIDES,33(2),298-306.
MLA Wang, Xin-Lei,et al."Apelin supports primary rat retinal Muller cells under chemical hypoxia and glucose deprivation".PEPTIDES 33.2(2012):298-306.
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