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学科主题: 临床医学
题名:
Liquid chromatographic-tandem mass spectrometric method for the quantitation of sildenafil in human plasma
作者: Wang, YW1; Wang, J1; Cui, YM1; Fawcett, JP1; Gu, JK1
关键词: sildenafil
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2005-12-15
DOI: 10.1016/j.jchromb.2005.09.008
卷: 828, 期:1-2, 页:118-121
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: METABOLITE UK-103,320 ; MS ; VIAGRA(R) ; COLUMN ; ASSAY ; RAT
英文摘要:

A method to determine sildenafil in human plasma involving liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. Sildenafil and the internal standard (I.S.), diazepam, are extracted from human plasma with ether-dichloromethane (3:2, v/v) at basic pH and analyzed by reversed-phase high-performance liquid chromatography (HPLC) using methanol-10 mM ammonium acetate pH 7.0 (85:15, v/v) as the mobile phase. Detection by electrospray positive ionization mass spectrometry in the multiple-reaction monitoring mode was linear over the concentration range 0.125-40.0 ng/ml. Intra- and inter-day precision of the assay at four concentrations within this range were 2.5-8.0%. The method was used to evaluate plasma concentration-time profiles in healthy volunteers given an oral dose of 20 mg sildenafil as a combination tablet also containing apomorphime. (c) 2005 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000233944100018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51135
Appears in Collections:北京大学第一临床医学院_药剂科_期刊论文

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作者单位: 1.Jilin Univ, Res Ctr Drug Metab, Coll Life Sci, Changchun 130023, Peoples R China
2.Peking Univ, Dept Pharm, Hosp 1, Beijing 100034, Peoples R China
3.Univ Otago, Sch Pharm, Dunedin, New Zealand

Recommended Citation:
Wang, YW,Wang, J,Cui, YM,et al. Liquid chromatographic-tandem mass spectrometric method for the quantitation of sildenafil in human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,828(1-2):118-121.
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