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Liquid chromatographic-tandem mass spectrometric method for the quantitation of sildenafil in human plasma
Wang, YW1; Wang, J1; Cui, YM1; Fawcett, JP1; Gu, JK1
关键词Sildenafil
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2005-12-15
DOI10.1016/j.jchromb.2005.09.008
828期:1-2页:118-121
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]METABOLITE UK-103,320 ; MS ; VIAGRA(R) ; COLUMN ; ASSAY ; RAT
英文摘要

A method to determine sildenafil in human plasma involving liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. Sildenafil and the internal standard (I.S.), diazepam, are extracted from human plasma with ether-dichloromethane (3:2, v/v) at basic pH and analyzed by reversed-phase high-performance liquid chromatography (HPLC) using methanol-10 mM ammonium acetate pH 7.0 (85:15, v/v) as the mobile phase. Detection by electrospray positive ionization mass spectrometry in the multiple-reaction monitoring mode was linear over the concentration range 0.125-40.0 ng/ml. Intra- and inter-day precision of the assay at four concentrations within this range were 2.5-8.0%. The method was used to evaluate plasma concentration-time profiles in healthy volunteers given an oral dose of 20 mg sildenafil as a combination tablet also containing apomorphime. (c) 2005 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000233944100018
引用统计
被引频次:42[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51135
专题北京大学第一临床医学院_药剂科
作者单位1.Jilin Univ, Res Ctr Drug Metab, Coll Life Sci, Changchun 130023, Peoples R China
2.Peking Univ, Dept Pharm, Hosp 1, Beijing 100034, Peoples R China
3.Univ Otago, Sch Pharm, Dunedin, New Zealand
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GB/T 7714
Wang, YW,Wang, J,Cui, YM,et al. Liquid chromatographic-tandem mass spectrometric method for the quantitation of sildenafil in human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,828(1-2):118-121.
APA Wang, YW,Wang, J,Cui, YM,Fawcett, JP,&Gu, JK.(2005).Liquid chromatographic-tandem mass spectrometric method for the quantitation of sildenafil in human plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,828(1-2),118-121.
MLA Wang, YW,et al."Liquid chromatographic-tandem mass spectrometric method for the quantitation of sildenafil in human plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 828.1-2(2005):118-121.
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