北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 药剂科  > 期刊论文
学科主题: 临床医学
题名:
Liquid chromatographic-tandem mass spectrometric method for the quantitation of sildenafil in human plasma
作者: Wang, YW1; Wang, J1; Cui, YM1; Fawcett, JP1; Gu, JK1
关键词: sildenafil
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2005-12-15
DOI: 10.1016/j.jchromb.2005.09.008
卷: 828, 期:1-2, 页:118-121
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: METABOLITE UK-103,320 ; MS ; VIAGRA(R) ; COLUMN ; ASSAY ; RAT
英文摘要:

A method to determine sildenafil in human plasma involving liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. Sildenafil and the internal standard (I.S.), diazepam, are extracted from human plasma with ether-dichloromethane (3:2, v/v) at basic pH and analyzed by reversed-phase high-performance liquid chromatography (HPLC) using methanol-10 mM ammonium acetate pH 7.0 (85:15, v/v) as the mobile phase. Detection by electrospray positive ionization mass spectrometry in the multiple-reaction monitoring mode was linear over the concentration range 0.125-40.0 ng/ml. Intra- and inter-day precision of the assay at four concentrations within this range were 2.5-8.0%. The method was used to evaluate plasma concentration-time profiles in healthy volunteers given an oral dose of 20 mg sildenafil as a combination tablet also containing apomorphime. (c) 2005 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000233944100018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51135
Appears in Collections:北京大学第一临床医学院_药剂科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Jilin Univ, Res Ctr Drug Metab, Coll Life Sci, Changchun 130023, Peoples R China
2.Peking Univ, Dept Pharm, Hosp 1, Beijing 100034, Peoples R China
3.Univ Otago, Sch Pharm, Dunedin, New Zealand

Recommended Citation:
Wang, YW,Wang, J,Cui, YM,et al. Liquid chromatographic-tandem mass spectrometric method for the quantitation of sildenafil in human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,828(1-2):118-121.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, YW]'s Articles
[Wang, J]'s Articles
[Cui, YM]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, YW]‘s Articles
[Wang, J]‘s Articles
[Cui, YM]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace