IR@PKUHSC  > 北京大学基础医学院  > 病理学系
学科主题基础医学
Impaired Intestinal Calcium Absorption in Protein 4.1R-deficient Mice Due to Altered Expression of Plasma Membrane Calcium ATPase 1b (PMCA1b)
Liu, Congrong1,2; Weng, Haibao1,3; Chen, Lixiang1,3; Yang, Shaomin1,2; Wang, Hua1,2; Debnath, Gargi1; Guo, Xinhua1; Wu, Liancheng1; Mohandas, Narla1; An, Xiuli1,3
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2013-04-19
DOI10.1074/jbc.M112.436659
288期:16页:11407-11415
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
资助者National Institutes of Health ; Beijing Municipal Natural Science Foundation of China ; Natural Science Foundation of China ; National Institutes of Health ; Beijing Municipal Natural Science Foundation of China ; Natural Science Foundation of China
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]CA2+ PUMP ; FUNCTIONAL-CHARACTERIZATION ; GENE FAMILY ; 4.1R ; CELLS ; BINDING ; ISOFORMS ; COMPLEX ; PHOSPHORYLATION ; CYTOSKELETON
英文摘要

Protein 4.1R was first identified in the erythrocyte membrane skeleton. It is now known that the protein is expressed in a variety of epithelial cell lines and in the epithelia of many tissues, including the small intestine. However, the physiological function of 4.1R in the epithelial cells of the small intestine has not so far been explored. Here, we show that 4.1R knock-out mice exhibited a significantly impaired small intestinal calcium absorption that resulted in secondary hyperparathyroidism as evidenced by increased serum 1,25-(OH)(2)-vitamin D-3 and parathyroid hormone levels, decreased serum calcium levels, hyperplasia of the parathyroid, and demineralization of the bones. 4.1R is located on the basolateral membrane of enterocytes, where it co-localizes with PMCA1b (plasma membrane calcium ATPase 1b). Expression of PMCA1b in enterocytes was decreased in 4.1(-/-) mice. 4.1R directly associated with PMCA1b, and the association involved the membrane-binding domain of 4.1R and the second intracellular loop and C terminus of PMCA1b. Our findings have enabled us to define a functional role for 4.1R in small intestinal calcium absorption through regulation of membrane expression of PMCA1b.

语种英语
所属项目编号DK26263 ; DK32094 ; 7082055 ; 81171905
资助者National Institutes of Health ; Beijing Municipal Natural Science Foundation of China ; Natural Science Foundation of China ; National Institutes of Health ; Beijing Municipal Natural Science Foundation of China ; Natural Science Foundation of China
WOS记录号WOS:000317915500043
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51141
Collection北京大学基础医学院_病理学系
作者单位1.New York Blood Ctr, Red Cell Physiol Lab, New York, NY 10065 USA
2.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China
3.Zhengzhou Univ, Dept Bioengn, Zhengzhou 450051, Peoples R China
Recommended Citation
GB/T 7714
Liu, Congrong,Weng, Haibao,Chen, Lixiang,et al. Impaired Intestinal Calcium Absorption in Protein 4.1R-deficient Mice Due to Altered Expression of Plasma Membrane Calcium ATPase 1b (PMCA1b)[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(16):11407-11415.
APA Liu, Congrong.,Weng, Haibao.,Chen, Lixiang.,Yang, Shaomin.,Wang, Hua.,...&An, Xiuli.(2013).Impaired Intestinal Calcium Absorption in Protein 4.1R-deficient Mice Due to Altered Expression of Plasma Membrane Calcium ATPase 1b (PMCA1b).JOURNAL OF BIOLOGICAL CHEMISTRY,288(16),11407-11415.
MLA Liu, Congrong,et al."Impaired Intestinal Calcium Absorption in Protein 4.1R-deficient Mice Due to Altered Expression of Plasma Membrane Calcium ATPase 1b (PMCA1b)".JOURNAL OF BIOLOGICAL CHEMISTRY 288.16(2013):11407-11415.
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