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学科主题: 基础医学
题名:
Impaired Intestinal Calcium Absorption in Protein 4.1R-deficient Mice Due to Altered Expression of Plasma Membrane Calcium ATPase 1b (PMCA1b)
作者: Liu, Congrong1,2; Weng, Haibao1,3; Chen, Lixiang1,3; Yang, Shaomin1,2; Wang, Hua1,2; Debnath, Gargi1; Guo, Xinhua1; Wu, Liancheng1; Mohandas, Narla1; An, Xiuli1,3
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2013-04-19
DOI: 10.1074/jbc.M112.436659
卷: 288, 期:16, 页:11407-11415
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: CA2+ PUMP ; FUNCTIONAL-CHARACTERIZATION ; GENE FAMILY ; 4.1R ; CELLS ; BINDING ; ISOFORMS ; COMPLEX ; PHOSPHORYLATION ; CYTOSKELETON
英文摘要:

Protein 4.1R was first identified in the erythrocyte membrane skeleton. It is now known that the protein is expressed in a variety of epithelial cell lines and in the epithelia of many tissues, including the small intestine. However, the physiological function of 4.1R in the epithelial cells of the small intestine has not so far been explored. Here, we show that 4.1R knock-out mice exhibited a significantly impaired small intestinal calcium absorption that resulted in secondary hyperparathyroidism as evidenced by increased serum 1,25-(OH)(2)-vitamin D-3 and parathyroid hormone levels, decreased serum calcium levels, hyperplasia of the parathyroid, and demineralization of the bones. 4.1R is located on the basolateral membrane of enterocytes, where it co-localizes with PMCA1b (plasma membrane calcium ATPase 1b). Expression of PMCA1b in enterocytes was decreased in 4.1(-/-) mice. 4.1R directly associated with PMCA1b, and the association involved the membrane-binding domain of 4.1R and the second intracellular loop and C terminus of PMCA1b. Our findings have enabled us to define a functional role for 4.1R in small intestinal calcium absorption through regulation of membrane expression of PMCA1b.

语种: 英语
所属项目编号: DK26263 ; DK32094 ; 7082055 ; 81171905
项目资助者: National Institutes of Health ; Beijing Municipal Natural Science Foundation of China ; Natural Science Foundation of China
WOS记录号: WOS:000317915500043
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51141
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.New York Blood Ctr, Red Cell Physiol Lab, New York, NY 10065 USA
2.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China
3.Zhengzhou Univ, Dept Bioengn, Zhengzhou 450051, Peoples R China

Recommended Citation:
Liu, Congrong,Weng, Haibao,Chen, Lixiang,et al. Impaired Intestinal Calcium Absorption in Protein 4.1R-deficient Mice Due to Altered Expression of Plasma Membrane Calcium ATPase 1b (PMCA1b)[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(16):11407-11415.
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