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C5a and its receptors in human anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis
Yuan, Jun1,2; Gou, Shen-Ju1; Huang, Jing1; Hao, Jian1; Chen, Min1; Zhao, Ming-Hui1
刊名ARTHRITIS RESEARCH & THERAPY
2012
DOI10.1186/ar3873
14期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Rheumatology
资助者Chinese 973 project ; National Natural Science Fund ; Chinese 973 project ; National Natural Science Fund
研究领域[WOS]Rheumatology
关键词[WOS]HUMAN POLYMORPHONUCLEAR LEUKOCYTES ; SYSTEMIC-LUPUS-ERYTHEMATOSUS ; TUBULAR EPITHELIAL-CELLS ; CHINESE PATIENTS ; COMPLEMENT ACTIVATION ; WEGENERS-GRANULOMATOSIS ; GLOMERULONEPHRITIS ; CLASSIFICATION ; AUTOANTIBODIES ; INHIBITION
英文摘要

Introduction: The complement system is crucial for the development of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). In particular, C5a plays a central role. In this study, plasma and urinary levels of C5a as well as renal C5a receptors (CD88 and C5L2) expression were investigated in patients with AAV.

Methods: Twenty-four patients with AAV in the active phase, 19 patients with AAV in the remission phase, and 20 patients with lupus nephritis (LN) were included. Plasma and urinary levels of C5a were measured with enzyme-linked immunosorbent assay (ELISA). The staining of CD88 and C5L2 in renal specimens was detected with immunohistochemistry.

Results: The level of plasma C5a was significantly higher in patients with AAV in the active phase than that in patients in remission, that in patients with LN, and that in normal controls. The urinary C5a level was significantly higher in patients with AAV in the active phase than that in patients in remission and that in normal controls, but not significantly different between patients with active AAV and patients with LN. The mean optical density of CD88 staining in the tubulointerstitium was significantly lower in AAV patients than that in normal controls (0.0052 +/- 0.0011 versus 0.029 +/- 0.0042; P = 0.005). The mean optical density of C5L2 in glomeruli was significantly higher in AAV patients than that in normal controls (0.013 +/- 0.0027 versus 0.0032 +/- 0.0006; P < 0.001). The mean optical density of CD88 staining closely correlated with the initial eGFR (r = 0.835; P < 0.001) in AAV patients. Double-labeling immunofluorescence assay suggested that CD88 did not express on neutrophils, monocytes, or macrophages, but C5L2 expressed on neutrophils (or monocytes) and macrophages.

Conclusion: The elevated plasma and urinary C5a levels indicated complement activation in human AAV. The level of renal CD88 expression could reflect the disease severity of ANCA-associated glomerulonephritis. CD88 expression was downregulated, and C5L2 was upregulated in ANCA-associated glomerulonephritis.

语种英语
所属项目编号2012CB517702 ; 30972733 ; 81021004
资助者Chinese 973 project ; National Natural Science Fund ; Chinese 973 project ; National Natural Science Fund
WOS记录号WOS:000314972600042
引用统计
被引频次:39[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51162
专题北京大学第一临床医学院
作者单位1.Peking Univ, Hosp 1, Dept Med,Minist Hlth China, Div Renal,Inst Nephrol,Key Lab Renal Dis, Beijing 100034, Peoples R China
2.Hubei Univ Tradit Chinese Med, Div Renal, Dept Internal Med, Hubei Prov Hosp Tradit Chinese Med,Dept Med, Wuhan 430061, Peoples R China
推荐引用方式
GB/T 7714
Yuan, Jun,Gou, Shen-Ju,Huang, Jing,et al. C5a and its receptors in human anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis[J]. ARTHRITIS RESEARCH &amp; THERAPY,2012,14(3).
APA Yuan, Jun,Gou, Shen-Ju,Huang, Jing,Hao, Jian,Chen, Min,&Zhao, Ming-Hui.(2012).C5a and its receptors in human anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis.ARTHRITIS RESEARCH & THERAPY,14(3).
MLA Yuan, Jun,et al."C5a and its receptors in human anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis".ARTHRITIS RESEARCH & THERAPY 14.3(2012).
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