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学科主题: 临床医学
题名:
C5a and its receptors in human anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis
作者: Yuan, Jun1,2; Gou, Shen-Ju1; Huang, Jing1; Hao, Jian1; Chen, Min1; Zhao, Ming-Hui1
刊名: ARTHRITIS RESEARCH & THERAPY
发表日期: 2012
DOI: 10.1186/ar3873
卷: 14, 期:3
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: HUMAN POLYMORPHONUCLEAR LEUKOCYTES ; SYSTEMIC-LUPUS-ERYTHEMATOSUS ; TUBULAR EPITHELIAL-CELLS ; CHINESE PATIENTS ; COMPLEMENT ACTIVATION ; WEGENERS-GRANULOMATOSIS ; GLOMERULONEPHRITIS ; CLASSIFICATION ; AUTOANTIBODIES ; INHIBITION
英文摘要:

Introduction: The complement system is crucial for the development of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). In particular, C5a plays a central role. In this study, plasma and urinary levels of C5a as well as renal C5a receptors (CD88 and C5L2) expression were investigated in patients with AAV.

Methods: Twenty-four patients with AAV in the active phase, 19 patients with AAV in the remission phase, and 20 patients with lupus nephritis (LN) were included. Plasma and urinary levels of C5a were measured with enzyme-linked immunosorbent assay (ELISA). The staining of CD88 and C5L2 in renal specimens was detected with immunohistochemistry.

Results: The level of plasma C5a was significantly higher in patients with AAV in the active phase than that in patients in remission, that in patients with LN, and that in normal controls. The urinary C5a level was significantly higher in patients with AAV in the active phase than that in patients in remission and that in normal controls, but not significantly different between patients with active AAV and patients with LN. The mean optical density of CD88 staining in the tubulointerstitium was significantly lower in AAV patients than that in normal controls (0.0052 +/- 0.0011 versus 0.029 +/- 0.0042; P = 0.005). The mean optical density of C5L2 in glomeruli was significantly higher in AAV patients than that in normal controls (0.013 +/- 0.0027 versus 0.0032 +/- 0.0006; P < 0.001). The mean optical density of CD88 staining closely correlated with the initial eGFR (r = 0.835; P < 0.001) in AAV patients. Double-labeling immunofluorescence assay suggested that CD88 did not express on neutrophils, monocytes, or macrophages, but C5L2 expressed on neutrophils (or monocytes) and macrophages.

Conclusion: The elevated plasma and urinary C5a levels indicated complement activation in human AAV. The level of renal CD88 expression could reflect the disease severity of ANCA-associated glomerulonephritis. CD88 expression was downregulated, and C5L2 was upregulated in ANCA-associated glomerulonephritis.

语种: 英语
所属项目编号: 2012CB517702 ; 30972733 ; 81021004
项目资助者: Chinese 973 project ; National Natural Science Fund
WOS记录号: WOS:000314972600042
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51162
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Med,Minist Hlth China, Div Renal,Inst Nephrol,Key Lab Renal Dis, Beijing 100034, Peoples R China
2.Hubei Univ Tradit Chinese Med, Div Renal, Dept Internal Med, Hubei Prov Hosp Tradit Chinese Med,Dept Med, Wuhan 430061, Peoples R China

Recommended Citation:
Yuan, Jun,Gou, Shen-Ju,Huang, Jing,et al. C5a and its receptors in human anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis[J]. ARTHRITIS RESEARCH &amp; THERAPY,2012,14(3).
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