IR@PKUHSC  > 北京大学第二临床医学院  > 眼科
学科主题临床医学
Expression of pro- and anti-angiogenic isoforms of VEGF in the mouse model of oxygen-induced retinopathy
Zhao, Min1; Shi, Xuan1; Liang, Jianhong1; Miao, Yifei2; Xie, Wankun1; Zhang, Yan1; Li, Xiaoxin1
关键词Vegf Vegfxxx Vegfxxxb Retinopathy Of Prematurity Neovascularization
刊名EXPERIMENTAL EYE RESEARCH
2011-12-01
DOI10.1016/j.exer.2011.10.013
93期:6页:921-926
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; SPLICE VARIANT ; VEGF(165)B ; CARCINOMA
英文摘要

Retinopathy of prematurity (ROP) has become one of the leading causes of blindness and visual loss in children over the last half century. Vascular Endothelial growth Factor (VEGF-A) is the principal stimulator of angiogenesis. Recently, it has been identified that VEGF was differentially spliced from Exons 8 to Exons 8a and 8b to form two families: the pro-angiogenic VEGFxxx family and the anti-angiogenic VEGFxxxb family. This alternate splicing produced VEGFxxxb proteins of the same length as VEGFxxx family, but with different C terminal amino acid sequences. VEGFxxxb appeared to be able to inhibit VEGFxxx-dependent angiogenesis. In our study, we investigated the protein expression course of VEGFxxx and VEGFxxxb by Western-blot in a mouse model of Oxygen-induced Retinopathy (OIR) from postnatal day 1 (P1) to postnatal day 21 (P21). We also analyzed the relative protein expression level of VEGF(165)b isoform in the OIR mouse model. We found that both VEGFxxx and VEGFxxxb were present in the mouse retina, among which. VEGF(164) and VEGF(165)b appeared to be predominant VEGFxxx and VEGFxxxb isoforms respectively in the mouse retina. We also found that the two family had different expression pattern correlated with neovascularization development and that the relative expression level of VEGF(165)b isoform switched during the neovascularization development in the OIR mouse model. In OIR group, the protein level of total VEGF isoforms (a mix of VEGF(164) and VEGF(165)b, detected by pan-VEGF antibody) continuously increased and peaked at P17 while VEGF(165)b continuously decreased from P9 which was well related with the vessel obliteration and neovascularization development in the mouse model of OIR. The neovascularization development correlates with an increase of total VEGF isoforms and the decrease of VEGF(165)b, indicating that there is a pro-angiogenic VEGF shift. Therefore, anti-angiogenic therapy that could alter the ratio of VEGFxxxb/VEGFxxx may be more effective. (C) 2011 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000297902600017
项目编号30801265 ; 2011CB510200
资助机构National Natural Science Foundation of China ; National Basic Research Program of China (973 Program)
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51164
专题北京大学第二临床医学院_眼科
作者单位1.Minist Educ, Key Lab Cardiovasc Sci, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Min,Shi, Xuan,Liang, Jianhong,et al. Expression of pro- and anti-angiogenic isoforms of VEGF in the mouse model of oxygen-induced retinopathy[J]. EXPERIMENTAL EYE RESEARCH,2011,93(6):921-926.
APA Zhao, Min.,Shi, Xuan.,Liang, Jianhong.,Miao, Yifei.,Xie, Wankun.,...&Li, Xiaoxin.(2011).Expression of pro- and anti-angiogenic isoforms of VEGF in the mouse model of oxygen-induced retinopathy.EXPERIMENTAL EYE RESEARCH,93(6),921-926.
MLA Zhao, Min,et al."Expression of pro- and anti-angiogenic isoforms of VEGF in the mouse model of oxygen-induced retinopathy".EXPERIMENTAL EYE RESEARCH 93.6(2011):921-926.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, Min]的文章
[Shi, Xuan]的文章
[Liang, Jianhong]的文章
百度学术
百度学术中相似的文章
[Zhao, Min]的文章
[Shi, Xuan]的文章
[Liang, Jianhong]的文章
必应学术
必应学术中相似的文章
[Zhao, Min]的文章
[Shi, Xuan]的文章
[Liang, Jianhong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。