北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 眼科  > 期刊论文
学科主题: 临床医学
题名:
Expression of pro- and anti-angiogenic isoforms of VEGF in the mouse model of oxygen-induced retinopathy
作者: Zhao, Min1; Shi, Xuan1; Liang, Jianhong1; Miao, Yifei2; Xie, Wankun1; Zhang, Yan1; Li, Xiaoxin1
关键词: VEGF ; VEGFxxx ; VEGFxxxb ; retinopathy of prematurity ; neovascularization
刊名: EXPERIMENTAL EYE RESEARCH
发表日期: 2011-12-01
DOI: 10.1016/j.exer.2011.10.013
卷: 93, 期:6, 页:921-926
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; SPLICE VARIANT ; VEGF(165)B ; CARCINOMA
英文摘要:

Retinopathy of prematurity (ROP) has become one of the leading causes of blindness and visual loss in children over the last half century. Vascular Endothelial growth Factor (VEGF-A) is the principal stimulator of angiogenesis. Recently, it has been identified that VEGF was differentially spliced from Exons 8 to Exons 8a and 8b to form two families: the pro-angiogenic VEGFxxx family and the anti-angiogenic VEGFxxxb family. This alternate splicing produced VEGFxxxb proteins of the same length as VEGFxxx family, but with different C terminal amino acid sequences. VEGFxxxb appeared to be able to inhibit VEGFxxx-dependent angiogenesis. In our study, we investigated the protein expression course of VEGFxxx and VEGFxxxb by Western-blot in a mouse model of Oxygen-induced Retinopathy (OIR) from postnatal day 1 (P1) to postnatal day 21 (P21). We also analyzed the relative protein expression level of VEGF(165)b isoform in the OIR mouse model. We found that both VEGFxxx and VEGFxxxb were present in the mouse retina, among which. VEGF(164) and VEGF(165)b appeared to be predominant VEGFxxx and VEGFxxxb isoforms respectively in the mouse retina. We also found that the two family had different expression pattern correlated with neovascularization development and that the relative expression level of VEGF(165)b isoform switched during the neovascularization development in the OIR mouse model. In OIR group, the protein level of total VEGF isoforms (a mix of VEGF(164) and VEGF(165)b, detected by pan-VEGF antibody) continuously increased and peaked at P17 while VEGF(165)b continuously decreased from P9 which was well related with the vessel obliteration and neovascularization development in the mouse model of OIR. The neovascularization development correlates with an increase of total VEGF isoforms and the decrease of VEGF(165)b, indicating that there is a pro-angiogenic VEGF shift. Therefore, anti-angiogenic therapy that could alter the ratio of VEGFxxxb/VEGFxxx may be more effective. (C) 2011 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30801265 ; 2011CB510200
项目资助者: National Natural Science Foundation of China ; National Basic Research Program of China (973 Program)
WOS记录号: WOS:000297902600017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51164
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Minist Educ, Key Lab Cardiovasc Sci, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China

Recommended Citation:
Zhao, Min,Shi, Xuan,Liang, Jianhong,et al. Expression of pro- and anti-angiogenic isoforms of VEGF in the mouse model of oxygen-induced retinopathy[J]. EXPERIMENTAL EYE RESEARCH,2011,93(6):921-926.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Min]'s Articles
[Shi, Xuan]'s Articles
[Liang, Jianhong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Min]‘s Articles
[Shi, Xuan]‘s Articles
[Liang, Jianhong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace