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Chemical fingerprint and metabolic fingerprint analysis of Danshen injection by HPLC-UV and HPLC-MS methods
Zhang, JL1; Cui, M1; He, Y1; Yu, HL1; Guo, DA1
关键词Chemical Fingerprint Metabolic Fingerprint Danshen Injection Salvia Miltiorrhiza Hplc-ms Technique
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2005-01-04
DOI10.1016/j.jpba.2004.09.009
36期:5页:1029-1035
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
英文摘要

HPLC-UV and HPLC-MS techniques were used in fingerprint analysis of Danshen injection and its raw materials (roots and rhizoma of Salvia miltiorrhiza). HPLC profiles of Danshen injections from a Chinese pharmaceutical factory and their raw, materials were established as their characteristic fingerprint and employed to assess their consistency and difference. To develop the representative fingerprint of Danshen injection, 10 batches of samples were analyzed under the same HPLC conditions. The results showed that 10 batches of Danshen injections had very similar HPLC fingerprints. To characterize the major constituents of Danshen injection for quality control, II major chromatographic peaks were characterized by their MS spectra and comparison with the reference standards. Through comparison of the HPLC profiles: of Danshen injection with its raw material, it was found that they are greatly different. which indicated the changes of major constituents in the course of preparation procedure. In addition, the rat′s plasma was analyzed by HPLC-MS technique after intravenous administration of Danshen injection at different time intervals to explore the in vivo metabolism of the major active constituents. Except for protocatechuic aldehyde, the major phenolic acids in Danshen injection appeared in rat′s plasma after intravenous administration. but quantity of each phenolic acids was very different from that in Danshen injection. With the administration time prolonged danshensu and salvianolic acid B disappeared quickly, salvianolic D. lithospermic acid and salvianolic A slowly decreased and maintained relatively high concentration after 30 min of intravenous administration. This indicated that polyphenolic acids were significant for biological acitivity of Danshen injection. It might be concluded that chemical fingerprint combined with metabolic fingerprint is a useful means to control the quality and to clarify the possible mechanism of action of herbal products. (C) 2004 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000226676600014
引用统计
被引频次:142[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51173
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Peking Univ, Modern Res Ctr Tradit Chinese Med, Beijing 100083, Peoples R China
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GB/T 7714
Zhang, JL,Cui, M,He, Y,et al. Chemical fingerprint and metabolic fingerprint analysis of Danshen injection by HPLC-UV and HPLC-MS methods[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2005,36(5):1029-1035.
APA Zhang, JL,Cui, M,He, Y,Yu, HL,&Guo, DA.(2005).Chemical fingerprint and metabolic fingerprint analysis of Danshen injection by HPLC-UV and HPLC-MS methods.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,36(5),1029-1035.
MLA Zhang, JL,et al."Chemical fingerprint and metabolic fingerprint analysis of Danshen injection by HPLC-UV and HPLC-MS methods".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 36.5(2005):1029-1035.
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