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VALIDATION OF A RAPID AND SIMPLE LC-MS/MS METHOD TO DETERMINE TRIMETAZIDINE IN RAT PLASMA
Xiong, Xin1,2; Yang, Li1,2; Zhai, Suodi1
关键词Lc-ms/ms Rapid Rat Plasma Simple Trimetazidine Validation
刊名JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
2011
DOI10.1080/10826076.2011.576298
34期:16页:1645-1653
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]MASS-SPECTROMETRY ; MS METHOD ; BIOEQUIVALENCE ; VOLUNTEERS
英文摘要

A rapid, simple, and sensitive LC-MS/MS method was developed and validated for the determination of trimetazidine in rat plasma, using lidocaine as the internal standard (IS). The chromatographic separation was accomplished on a Atlantis dC(18) column (150 x 2.1 mm, 5 mu m) with the mobile phase consisting of acetonitrile and 10 mM ammonium formate (3:7, v/v) (pH 3.05, adjusted with formic acid). Plasma samples were deproteined with acetonitrile (1:3) and 3 mu L of the supernatant was injected into the system. The retention times of trimetazidine and IS were approximately 1.45 min and 2.10 min, respectively. Calibration curves were linear over the concentration range of 0.5-500 ng/mL (1000 fold). The intra-and inter-batch precision, accuracy, and the relative standard deviation were all < 15%. This method was successfully applied to determine trimetazidine concentrations in rat plasma.

语种英语
WOS记录号WOS:000296231300002
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51174
专题北京大学第三临床医学院_药剂科
作者单位1.Peking Univ, Dept Pharm, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Therapeut Drug Monitoring & Clin Toxicol Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Xiong, Xin,Yang, Li,Zhai, Suodi. VALIDATION OF A RAPID AND SIMPLE LC-MS/MS METHOD TO DETERMINE TRIMETAZIDINE IN RAT PLASMA[J]. JOURNAL OF LIQUID CHROMATOGRAPHY &amp; RELATED TECHNOLOGIES,2011,34(16):1645-1653.
APA Xiong, Xin,Yang, Li,&Zhai, Suodi.(2011).VALIDATION OF A RAPID AND SIMPLE LC-MS/MS METHOD TO DETERMINE TRIMETAZIDINE IN RAT PLASMA.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,34(16),1645-1653.
MLA Xiong, Xin,et al."VALIDATION OF A RAPID AND SIMPLE LC-MS/MS METHOD TO DETERMINE TRIMETAZIDINE IN RAT PLASMA".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 34.16(2011):1645-1653.
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