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A Cell Permeable NPE Caged ADP-Ribose for Studying TRPM2
Yu, Peilin1,2,3; Wang, Qian1; Zhang, Li-He2; Lee, Hon-Cheung2; Zhang, Liangren2; Yue, Jianbo1
刊名PLOS ONE
2012-12-07
DOI10.1371/journal.pone.0051028
7期:12
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Research Grant Council (RGC) ; National Natural Science Foundation of China (NSFC)/RGC grant from Hong Kong ; NSFC ; Leukemia and Lymphoma Society of America ; Research Grant Council (RGC) ; National Natural Science Foundation of China (NSFC)/RGC grant from Hong Kong ; NSFC ; Leukemia and Lymphoma Society of America
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]OXIDATIVE STRESS ; ION-CHANNEL ; INTRACELLULAR CALCIUM ; INSULIN-SECRETION ; BIPOLAR DISORDER ; CATION CHANNELS ; BETA-CELLS ; ACTIVATION ; ZINC ; CA2+
英文摘要

Transient potential receptor melastatin-2 (TRPM2) is a non-selective Ca2+-permeable cation channel of the TRPM channel subfamily and is mainly activated by intracellular adenosine diphosphate ribose (ADPR). Here we synthesized a 1-(2-nitrophenyl) ethyl caged ADPR (NPE-ADPR) and found that uncaging of NPE-ADPR efficiently stimulated Ca2+, Mg2+, and Zn2+ influx in a concentration-dependent manner in intact human Jurkat T-lymphocytes. The cation influx was inhibited by inhibitors or knockdown of TRPM2. Likewise, uncaging of NPE-ADPR markedly induced cation entry in HEK 293 cells that overexpress TRPM2. As expected, high temperature increased the ability of the photolyzed NPE-ADPR to induce cation entry, whereas acidic pH inhibited. Moreover, the absence of extracellular Ca2+ significantly inhibited Mg2+ and Zn2+ influx after uncaging NPE-ADPR. On the other hand, the absence of extracellular Na+ or Mg2+ had no effect on photolyzed NPE-ADPR induced Ca2+ entry. Taken together, our results indicated that NPE-ADPR is a cell permeable ADPR analogue that is useful for studying TRPM2-mediated cation entry in intact cells.

语种英语
所属项目编号HKU 784710M ; HKU 782709M ; HKU 785911M ; N_HKU 737/09 ; 20910094
资助者Research Grant Council (RGC) ; National Natural Science Foundation of China (NSFC)/RGC grant from Hong Kong ; NSFC ; Leukemia and Lymphoma Society of America ; Research Grant Council (RGC) ; National Natural Science Foundation of China (NSFC)/RGC grant from Hong Kong ; NSFC ; Leukemia and Lymphoma Society of America
WOS记录号WOS:000312064100053
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51187
Collection北京大学药学院
作者单位1.Univ Hong Kong, Dept Physiol, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
3.Zhejiang Univ, Sch Publ Hlth, Dept Toxicol, Hangzhou 310003, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Yu, Peilin,Wang, Qian,Zhang, Li-He,et al. A Cell Permeable NPE Caged ADP-Ribose for Studying TRPM2[J]. PLOS ONE,2012,7(12).
APA Yu, Peilin,Wang, Qian,Zhang, Li-He,Lee, Hon-Cheung,Zhang, Liangren,&Yue, Jianbo.(2012).A Cell Permeable NPE Caged ADP-Ribose for Studying TRPM2.PLOS ONE,7(12).
MLA Yu, Peilin,et al."A Cell Permeable NPE Caged ADP-Ribose for Studying TRPM2".PLOS ONE 7.12(2012).
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