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Genotoxic evaluation of titanium dioxide nanoparticles in vivo and in vitro
Chen, Zhangjian; Wang, Yun; Ba, Te; Li, Yang; Pu, Ji; Chen, Tian; Song, Yanshuang; Gu, Yongen; Qian, Qin; Yang, Jinglin; Jia, Guang
关键词Titanium Dioxide Nanoparticles Genotoxicity Genetic Endpoint Chromosome Damage Dna Damage Gene Mutation
刊名TOXICOLOGY LETTERS
2014-05-02
DOI10.1016/j.toxlet.2014.02.020
226期:3页:314-319
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]TIO2 ; CELLS ; NANOMATERIALS ; ASSAY ; MICE ; NANOTOXICOLOGY ; CYTOTOXICITY ; LYMPHOCYTES ; PARTICLES ; TOXICITY
英文摘要

With the extensive application of titanium dioxide (TiO2) nanoparticles (NPs) in food industry, there is a rising debate concerning the possible risk associated with exposure to TiO2 NPs. The purpose of this study is to evaluate the genotoxicity of TiO2 NPs using in vivo and in vitro test systems. In vivo study, the adult male Sprague-Dawley rats were exposed to anatase TiO2 NPs (75 +/- 15 nm) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight every day for 30 days. The gamma-H(2)AX assay showed TiO2 NPs could induce DNA double strand breaks in bone marrow cells after oral administration. However, the micronucleus test revealed that the oral-exposed TiO2 NPs did not cause damage to chromosomes or mitotic apparatus observably in rat bone marrow cells. In vitro study, Chinese hamster lung fibroblasts (V79 cells) were exposed to TiO2 NPs at the dose of 0, 5, 10, 20, 50 and 100 mu g/mL. Significant decreases in cell viability were detected in all the treated groups after 24 h and 48 h exposure. Significant DNA damage was only observed at the concentration of 100 mu g/mL after 24 h treatment using the comet assay. The obvious gene mutation was observed at the concentration of 20 and 100 mu g/mL after 2 h treatment using hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene mutation assay. This study presented a comprehensive genotoxic evaluation of TiO2 NPs, and TiO2 NPs were shown to be genotoxic both in vivo and in vitro tests. The gene mutation and DNA strand breaks seem to be more sensitive genetic endpoints for the detection of TiO2 NPs induced genotoxic effects. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000334846300007
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51199
专题北京大学公共卫生学院
北京大学医学部管理机构_科学研究处
北京大学公共卫生学院_公共卫生学院
北京大学第二临床医学院_党委院长办公室
作者单位Peking Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Chen, Zhangjian,Wang, Yun,Ba, Te,et al. Genotoxic evaluation of titanium dioxide nanoparticles in vivo and in vitro[J]. TOXICOLOGY LETTERS,2014,226(3):314-319.
APA Chen, Zhangjian.,Wang, Yun.,Ba, Te.,Li, Yang.,Pu, Ji.,...&Jia, Guang.(2014).Genotoxic evaluation of titanium dioxide nanoparticles in vivo and in vitro.TOXICOLOGY LETTERS,226(3),314-319.
MLA Chen, Zhangjian,et al."Genotoxic evaluation of titanium dioxide nanoparticles in vivo and in vitro".TOXICOLOGY LETTERS 226.3(2014):314-319.
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