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学科主题: 公共卫生
题名:
Genotoxic evaluation of titanium dioxide nanoparticles in vivo and in vitro
作者: Chen, Zhangjian; Wang, Yun; Ba, Te; Li, Yang; Pu, Ji; Chen, Tian; Song, Yanshuang; Gu, Yongen; Qian, Qin; Yang, Jinglin; Jia, Guang
关键词: Titanium dioxide nanoparticles ; Genotoxicity ; Genetic endpoint ; Chromosome damage ; DNA damage ; Gene mutation
刊名: TOXICOLOGY LETTERS
发表日期: 2014-05-02
DOI: 10.1016/j.toxlet.2014.02.020
卷: 226, 期:3, 页:314-319
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: TIO2 ; CELLS ; NANOMATERIALS ; ASSAY ; MICE ; NANOTOXICOLOGY ; CYTOTOXICITY ; LYMPHOCYTES ; PARTICLES ; TOXICITY
英文摘要:

With the extensive application of titanium dioxide (TiO2) nanoparticles (NPs) in food industry, there is a rising debate concerning the possible risk associated with exposure to TiO2 NPs. The purpose of this study is to evaluate the genotoxicity of TiO2 NPs using in vivo and in vitro test systems. In vivo study, the adult male Sprague-Dawley rats were exposed to anatase TiO2 NPs (75 +/- 15 nm) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight every day for 30 days. The gamma-H(2)AX assay showed TiO2 NPs could induce DNA double strand breaks in bone marrow cells after oral administration. However, the micronucleus test revealed that the oral-exposed TiO2 NPs did not cause damage to chromosomes or mitotic apparatus observably in rat bone marrow cells. In vitro study, Chinese hamster lung fibroblasts (V79 cells) were exposed to TiO2 NPs at the dose of 0, 5, 10, 20, 50 and 100 mu g/mL. Significant decreases in cell viability were detected in all the treated groups after 24 h and 48 h exposure. Significant DNA damage was only observed at the concentration of 100 mu g/mL after 24 h treatment using the comet assay. The obvious gene mutation was observed at the concentration of 20 and 100 mu g/mL after 2 h treatment using hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene mutation assay. This study presented a comprehensive genotoxic evaluation of TiO2 NPs, and TiO2 NPs were shown to be genotoxic both in vivo and in vitro tests. The gene mutation and DNA strand breaks seem to be more sensitive genetic endpoints for the detection of TiO2 NPs induced genotoxic effects. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000334846300007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51199
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: Peking Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth Sci, Beijing 100191, Peoples R China

Recommended Citation:
Chen, Zhangjian,Wang, Yun,Ba, Te,et al. Genotoxic evaluation of titanium dioxide nanoparticles in vivo and in vitro[J]. TOXICOLOGY LETTERS,2014,226(3):314-319.
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