IR@PKUHSC  > 北京大学药学院
学科主题药学
Efficient in Vitro siRNA Delivery and Intramuscular Gene Silencing Using PEG-Modified PAMAM Dendrimers
Tang, Yin1; Li, Yang-Bing2; Wang, Bo2; Lin, Ri-Yuan2; van Dongen, Mallory3; Zurcher, Danielle M.3; Gu, Xiao-Yan2; Holl, Mark M. Banaszak3; Liu, George1; Qi, Rong1
关键词Peg-conjugated Pamam Dendrimer Cytotoxicity Rnase Stability In Vitro And Intramuscular Sirna Delivery Gene Silencing Efficiency
刊名MOLECULAR PHARMACEUTICS
2012-06-01
DOI10.1021/mp3001364
9期:6页:1812-1821
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]SMALL INTERFERING RNA ; NONVIRAL VECTORS ; VIVO ; CELLS ; GENERATION ; ANTISENSE
英文摘要

Although siRNA techniques have been broadly applied as a tool for gene knockdown, substantial challenges remain in achieving efficient delivery and in vivo efficacy. In particular, the low efficiency of target gene silencing in vivo is a critical limiting step to the clinical application of siRNA therapies. Poly(arnidoamine) (PAMAM) dendrimers are widely used as carriers for drug and gene delivery; however, in vivo siRNA delivery by PAMAM dendrimers remains to be carefully investigated. In this study, the effectiveness of G5 and G6 PAMAM dendrimers with 8% of their surface amines conjugated to MPEG-5000 was studied for siRNA delivery in vitro and for intramuscular in vivo delivery in mice. The results from the PEG-modified dendrimers were compared to the results from the parent dendrimers as well as Lipofectamine 2000 and INTERFERin. Both PEG-modifed dendrimers protect the siRNA from being digested by RNase and gave high transfection efficiency for FITC-labeled siRNA in the primary vascular smooth muscle cells (VSMC) and mouse peritoneal macrophages. The PEG-modified dendrimers achieved knockdown of both plasmid (293A cells) and adenovirus-mediated green fluorescence protein (GFP) expression (Cos7 cells) in vitro with efficiency similar to that shown for Lipofectamine 2000. We further demonstrated in vivo that intramuscular delivery of GFP-siRNA using PEG-modified dendrimer significantly suppressed GFP expression in both transiently adenovirus infected C57BL/6 mice and GFP transgenic mice.

语种英语
WOS记录号WOS:000304728700027
项目编号30500197 ; 30971241 ; 2009CB930300 ; RO1-EB005028
资助机构National Natural Science Foundation of China ; National Basic Research Program of China ; National Institute of Biomedical Imaging and Bioengineering
引用统计
被引频次:66[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51205
专题北京大学药学院
北京大学基础医学院
作者单位1.Peking Univ, Peking Univ Inst Cardiovasc Sci, Peking Univ Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Peking Univ Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
3.Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA
推荐引用方式
GB/T 7714
Tang, Yin,Li, Yang-Bing,Wang, Bo,et al. Efficient in Vitro siRNA Delivery and Intramuscular Gene Silencing Using PEG-Modified PAMAM Dendrimers[J]. MOLECULAR PHARMACEUTICS,2012,9(6):1812-1821.
APA Tang, Yin.,Li, Yang-Bing.,Wang, Bo.,Lin, Ri-Yuan.,van Dongen, Mallory.,...&Qi, Rong.(2012).Efficient in Vitro siRNA Delivery and Intramuscular Gene Silencing Using PEG-Modified PAMAM Dendrimers.MOLECULAR PHARMACEUTICS,9(6),1812-1821.
MLA Tang, Yin,et al."Efficient in Vitro siRNA Delivery and Intramuscular Gene Silencing Using PEG-Modified PAMAM Dendrimers".MOLECULAR PHARMACEUTICS 9.6(2012):1812-1821.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Tang, Yin]的文章
[Li, Yang-Bing]的文章
[Wang, Bo]的文章
百度学术
百度学术中相似的文章
[Tang, Yin]的文章
[Li, Yang-Bing]的文章
[Wang, Bo]的文章
必应学术
必应学术中相似的文章
[Tang, Yin]的文章
[Li, Yang-Bing]的文章
[Wang, Bo]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。