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学科主题: 药学
题名:
Simultaneous determination and pharmacokinetic analysis of seven alkaloids and two flavonoids from rat plasma by HPLC-DAD after oral administration of Wuzhuyu decoction
作者: Hu, Chuan-Qin1; Li, Fei1; Yang, Xiu-Wei1
关键词: Wuzhuyu decoction ; pharmacokinetics ; HPLC-DAD ; alkaloids ; flavonoids
刊名: JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
发表日期: 2012
DOI: 10.1080/10286020.2012.656093
卷: 14, 期:4, 页:370-381
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Applied ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; RUTAECARPINE ; EVODIAMINE ; CYTOCHROME-P450 ; EVOCARPINE ; METABOLITE ; SERUM
英文摘要:

A simple and sensitive high-performance liquid chromatographic method was developed for the simultaneous determination and pharmacokinetic analysis of seven alkaloids dehydroevodiamine (DHED), 10-hydroxyrutaecarpine (HDR), evodiamine (EDM), rutaecarpine (RCP), 1-methyl-2-n-nonyl-4(1H)quinolone (MNQ), evocarpine (ECP), and dihydroevocarpine (DHE), and two flavonoids isorhamnetin-7-O-rutinoside (RIM) and diosmetin-7-O-beta-D-glucopyranoside (GRD) in rat plasma after oral administration of Wuzhuyu decoction. The flow rate was kept at 1.0 ml/min and the detection wavelength was set at 300 nm. The calibration curves were linear in the range of 0.5013-30.076 mu g/ml for DHED, 0.2161-21.608 mu g/ml for RIM, 0.161-12.876 mu g/ml for HDR, 0.2146-21.457 mu g/ml for GRD, 2.0464-40.928 mu g/ml for EDM, 1.0398-31.194 mu g/ml for RCP, 0.5970-35.818 mu g/ml for MNQ, 0.8371-20.928 mu g/ml for ECP, and 0.5167-31.003 mu g/ml for DHE. The precision (relative standard deviation (RSD), %) for all was less than 10% and the accuracy (relative error (RE), %) was within +/-10%. The results demonstrated that the assay had remarkable reproducibility with acceptable accuracy and precision. The lower limit of quantifications for the compounds in plasma ranged from 0.12 to 0.23 mu g/ml and the lower limit of detections ranged from 0.024 to 0.076 mu g/ml. This validated method has been successfully applied in the pharmacokinetics study of seven alkaloids and two flavonoids after orally administrating the Wuzhuyu decoction to rats.

语种: 英语
所属项目编号: 2006BAI08B03-09 ; 2006BAI06A01-02 ; 2002AA2Z343C ; 2004AA2Z3783 ; Z0004105040311
项目资助者: National Sciences and Technology Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Special-Purpose Science Foundation of China
WOS记录号: WOS:000303567900012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51208
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100191, Peoples R China

Recommended Citation:
Hu, Chuan-Qin,Li, Fei,Yang, Xiu-Wei. Simultaneous determination and pharmacokinetic analysis of seven alkaloids and two flavonoids from rat plasma by HPLC-DAD after oral administration of Wuzhuyu decoction[J]. JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH,2012,14(4):370-381.
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