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Simultaneous determination and pharmacokinetic analysis of seven alkaloids and two flavonoids from rat plasma by HPLC-DAD after oral administration of Wuzhuyu decoction
Hu, Chuan-Qin1; Li, Fei1; Yang, Xiu-Wei1
关键词Wuzhuyu Decoction Pharmacokinetics Hplc-dad Alkaloids Flavonoids
刊名JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
2012
DOI10.1080/10286020.2012.656093
14期:4页:370-381
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Applied ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; RUTAECARPINE ; EVODIAMINE ; CYTOCHROME-P450 ; EVOCARPINE ; METABOLITE ; SERUM
英文摘要

A simple and sensitive high-performance liquid chromatographic method was developed for the simultaneous determination and pharmacokinetic analysis of seven alkaloids dehydroevodiamine (DHED), 10-hydroxyrutaecarpine (HDR), evodiamine (EDM), rutaecarpine (RCP), 1-methyl-2-n-nonyl-4(1H)quinolone (MNQ), evocarpine (ECP), and dihydroevocarpine (DHE), and two flavonoids isorhamnetin-7-O-rutinoside (RIM) and diosmetin-7-O-beta-D-glucopyranoside (GRD) in rat plasma after oral administration of Wuzhuyu decoction. The flow rate was kept at 1.0 ml/min and the detection wavelength was set at 300 nm. The calibration curves were linear in the range of 0.5013-30.076 mu g/ml for DHED, 0.2161-21.608 mu g/ml for RIM, 0.161-12.876 mu g/ml for HDR, 0.2146-21.457 mu g/ml for GRD, 2.0464-40.928 mu g/ml for EDM, 1.0398-31.194 mu g/ml for RCP, 0.5970-35.818 mu g/ml for MNQ, 0.8371-20.928 mu g/ml for ECP, and 0.5167-31.003 mu g/ml for DHE. The precision (relative standard deviation (RSD), %) for all was less than 10% and the accuracy (relative error (RE), %) was within +/-10%. The results demonstrated that the assay had remarkable reproducibility with acceptable accuracy and precision. The lower limit of quantifications for the compounds in plasma ranged from 0.12 to 0.23 mu g/ml and the lower limit of detections ranged from 0.024 to 0.076 mu g/ml. This validated method has been successfully applied in the pharmacokinetics study of seven alkaloids and two flavonoids after orally administrating the Wuzhuyu decoction to rats.

语种英语
WOS记录号WOS:000303567900012
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51208
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Hu, Chuan-Qin,Li, Fei,Yang, Xiu-Wei. Simultaneous determination and pharmacokinetic analysis of seven alkaloids and two flavonoids from rat plasma by HPLC-DAD after oral administration of Wuzhuyu decoction[J]. JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH,2012,14(4):370-381.
APA Hu, Chuan-Qin,Li, Fei,&Yang, Xiu-Wei.(2012).Simultaneous determination and pharmacokinetic analysis of seven alkaloids and two flavonoids from rat plasma by HPLC-DAD after oral administration of Wuzhuyu decoction.JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH,14(4),370-381.
MLA Hu, Chuan-Qin,et al."Simultaneous determination and pharmacokinetic analysis of seven alkaloids and two flavonoids from rat plasma by HPLC-DAD after oral administration of Wuzhuyu decoction".JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH 14.4(2012):370-381.
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