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学科主题: 基础医学
题名:
Nonstructural 5A gene variability of hepatitis C virus (HCV) during a 10-year follow up
作者: Fan, WM1; Zhu, WF1; Wei, L1; Wang, QX1; Yin, LM1; Du, SC1; Zhuang, H1
关键词: hepatitis C virus ; nonstructural region 5 ; interferon sensitivity-determining region ; sequence analysis
刊名: JOURNAL OF GASTROENTEROLOGY
发表日期: 2005
DOI: 10.1007/s00535-004-1446-2
卷: 40, 期:1, 页:43-51
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: PKR PROTEIN-KINASE ; SENSITIVITY-DETERMINING REGION ; GENOTYPE 1B ; TRANSCRIPTIONAL ACTIVATOR ; HEPATOCELLULAR-CARCINOMA ; INTERFERON RESISTANCE ; CHRONIC INFECTION ; NATURAL COURSE ; NON-A ; NS5A
英文摘要:

Background. Seven patients with chronic hepatitis C virus (HCV) infection were followed up for 10 years in order to analyze the molecular evolution of the HCV nonstructural 5A (NS5A) gene. Methods. Serum samples were obtained from seven patients with anti-HCV who were HCV-RNA positive. The HCV NS5A region, including the interferon sensitivity-determining region (ISDR), protein kinase R binding domain (PKR-BD), and V3 was amplified and cloned, and then sequenced. The nucleotide sequences of the region at the beginning and at the end of the 10 years were aligned with the CLUSTAL W program, version 1.8, and the corresponding amino-acid sequences were deduced. Results. The three serine residues at positions 2197, 2201, and 2204, suggested to be important for hyperphosphorylation of NS5A, were highly conserved in different patients and were within the quasispecies of each patient. The wild-type ISDR or minimally mutated strain was dominant in all the patients and the number of quasispecies decreased over time. Conclusions. Our findings showed that the functional domains of NS5A were generally conserved over an interval of 10 years. Quasispecies distribution was variable and distinctly clustered over time during the natural course of HCV infection. These results may contribute to better understanding of HCV natural infection.

语种: 英语
WOS记录号: WOS:000226809600006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51209
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100871, Peoples R China

Recommended Citation:
Fan, WM,Zhu, WF,Wei, L,et al. Nonstructural 5A gene variability of hepatitis C virus (HCV) during a 10-year follow up[J]. JOURNAL OF GASTROENTEROLOGY,2005,40(1):43-51.
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