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学科主题: 临床医学
题名:
Identification of active and quiescent adipose vascular stromal cells
作者: Lin, Guiting1; Xin, Zhongcheng2; Zhang, Haiyang1; Banie, Lia1; Wang, Guifang1; Qiu, Xuefeng1; Ning, Hongxiu1; Lue, Tom F.1; Lin, Ching-Shwun1
关键词: adipose-derived stem cells ; active and quiescent stem cells ; vascular stem cells
刊名: CYTOTHERAPY
发表日期: 2012-02-01
DOI: 10.3109/14653249.2011.627918
卷: 14, 期:2, 页:240-246
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology ; Hematology ; Medicine, Research & Experimental
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Hematology ; Research & Experimental Medicine
关键词[WOS]: LABEL-RETAINING CELLS ; ADULT STEM-CELLS ; PHENOTYPE ; LOCATION ; TRACKING ; CULTURE ; TISSUE
英文摘要:

Background aims. Recent studies have demonstrated the existence of both active and quiescent stem cells in bone marrow, hair follicle and intestine. We attempted to identify active and quiescent vascular stromal cells (VSC) in adipose tissue. Methods. For identification of active VSC, adult rats were injected intraperitoneally with thymidine analog 5-ethynyl-2-deoxyuridine (EdU) and their subcutaneous tissue harvested 3 days later. For identification of quiescent VSC, newborn rats were injected intraperitoneally with EdU and their subcutaneous tissue harvested 9 weeks later. The harvested adipose tissues were examined for the co-localization of EdU with VSC marker CD34, smooth muscle marker SMA, endothelial marker RECA and pericyte marker CD140b. Results. In adult rat adipose tissues harvested 3 days after EdU injection, there were 28.80 +/- 8.70 (mean +/- SD) EdU

语种: 英语
所属项目编号: DK64538 ; DK045370 ; DK069655
项目资助者: Arthur Rock Foundation ; National Institutes of Health
WOS记录号: WOS:000299086500014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51211
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Androl Ctr, Beijing 100871, Peoples R China
2.Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA

Recommended Citation:
Lin, Guiting,Xin, Zhongcheng,Zhang, Haiyang,et al. Identification of active and quiescent adipose vascular stromal cells[J]. CYTOTHERAPY,2012,14(2):240-246.
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