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IR@PKUHSC  > 北京大学第二临床医学院  > 计划生育与生殖医学科  > 期刊论文
学科主题: 临床医学
题名:
Pluripotin Combined with Leukemia Inhibitory Factor Greatly Promotes the Derivation of Embryonic Stem Cell Lines from Refractory Strains
作者: Yang, Weifeng1; Wei, Wei1,3; Shi, Cheng1,2; Zhu, Jinliang4; Ying, Wenqin4; Shen, Yan1; Ye, Xin1; Fang, Lingling4; Duo, Shuguang1; Che, Jie1; Shen, Huan2; Ding, Sheng5,6; Deng, Hongkui1,3
关键词: refractory mouse strains ; embryonic stem cell isolation ; efficiency ; pluripotin
刊名: STEM CELLS
发表日期: 2009
DOI: 10.1634/stemcells.2008-0974
卷: 27, 期:2, 页:383-389
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]: SELF-RENEWAL ; EFFICIENT DERIVATION ; IMPROVED GENERATION ; MOUSE STRAINS ; ESTABLISHMENT ; CULTURE ; DIFFERENCE
英文摘要:

Most mouse embryonic stem (ES) cells are derived from a 129 or C57BL/6 background, whereas the derivation efficiency of ES cells is extremely low on certain refractory types of background for which ES cells are highly desired. Here we report an optimized, highly efficient protocol by combining pluripotin, a small molecule, and leukemia inhibitory factor (LIF) for the derivation of mouse ES cells. With this method, we successfully isolated ES cell lines from five strains of mice, with an efficiency of 57% for NOD-scid, 63% for SCID beige, 80% for CD-1, and 100% for two F1 strains from C57BL/6 x CD-1. By tracking the Oct4-positive cells in the Oct4-green fluorescent protein embryos in the process of ES cell isolation, we found that pluripotin combined with LIF improved the efficiency of ES cell isolation by selectively maintaining the Oct4-positive cells in the outgrowth. To our knowledge, this is the first report of ES cells being efficiently derived from immunodeficient mice on refractory backgrounds (NOD-scid on a NOD background and SCID beige on a BALB/c background). STEM CELLS 2009; 27: 383-389

语种: 英语
所属项目编号: 2007CB947900 ; 30421004 ; 37871
项目资助者: National Basic Research Program for China ; Gongjian Project of the Beijing Municipal Education Commission ; National Natural Science Foundation of China ; Bill &amp ; Melinda Gates Foundation ; 111 Project
WOS记录号: WOS:000263719500013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51212
Appears in Collections:北京大学第二临床医学院_计划生育与生殖医学科_期刊论文

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作者单位: 1.Beijing Vital River Lab Anim Inc, Beijing, Peoples R China
2.Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
3.Peking Univ, Coll Life Sci, Lab Stem Cell & Generat Biol, Beijing 100871, Peoples R China
4.Peking Univ, Reprod Med Ctr, Peoples Hosp, Beijing 100871, Peoples R China
5.Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biothechnol, Lab Chem Genom, Shenzhen, Peoples R China
6.Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

Recommended Citation:
Yang, Weifeng,Wei, Wei,Shi, Cheng,et al. Pluripotin Combined with Leukemia Inhibitory Factor Greatly Promotes the Derivation of Embryonic Stem Cell Lines from Refractory Strains[J]. STEM CELLS,2009,27(2):383-389.
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