学科主题临床医学
Pluripotin Combined with Leukemia Inhibitory Factor Greatly Promotes the Derivation of Embryonic Stem Cell Lines from Refractory Strains
Yang, Weifeng1; Wei, Wei1,3; Shi, Cheng1,2; Zhu, Jinliang4; Ying, Wenqin4; Shen, Yan1; Ye, Xin1; Fang, Lingling4; Duo, Shuguang1; Che, Jie1; Shen, Huan2; Ding, Sheng5,6; Deng, Hongkui1,3
关键词Refractory Mouse Strains Embryonic Stem Cell Isolation Efficiency Pluripotin
刊名STEM CELLS
2009
DOI10.1634/stemcells.2008-0974
27期:2页:383-389
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]SELF-RENEWAL ; EFFICIENT DERIVATION ; IMPROVED GENERATION ; MOUSE STRAINS ; ESTABLISHMENT ; CULTURE ; DIFFERENCE
英文摘要

Most mouse embryonic stem (ES) cells are derived from a 129 or C57BL/6 background, whereas the derivation efficiency of ES cells is extremely low on certain refractory types of background for which ES cells are highly desired. Here we report an optimized, highly efficient protocol by combining pluripotin, a small molecule, and leukemia inhibitory factor (LIF) for the derivation of mouse ES cells. With this method, we successfully isolated ES cell lines from five strains of mice, with an efficiency of 57% for NOD-scid, 63% for SCID beige, 80% for CD-1, and 100% for two F1 strains from C57BL/6 x CD-1. By tracking the Oct4-positive cells in the Oct4-green fluorescent protein embryos in the process of ES cell isolation, we found that pluripotin combined with LIF improved the efficiency of ES cell isolation by selectively maintaining the Oct4-positive cells in the outgrowth. To our knowledge, this is the first report of ES cells being efficiently derived from immunodeficient mice on refractory backgrounds (NOD-scid on a NOD background and SCID beige on a BALB/c background). STEM CELLS 2009; 27: 383-389

语种英语
WOS记录号WOS:000263719500013
项目编号2007CB947900 ; 30421004 ; 37871
资助机构National Basic Research Program for China ; Gongjian Project of the Beijing Municipal Education Commission ; National Natural Science Foundation of China ; Bill &amp ; Melinda Gates Foundation ; 111 Project
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51212
专题北京大学第二临床医学院_计划生育与生殖医学科
作者单位1.Beijing Vital River Lab Anim Inc, Beijing, Peoples R China
2.Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
3.Peking Univ, Coll Life Sci, Lab Stem Cell & Generat Biol, Beijing 100871, Peoples R China
4.Peking Univ, Reprod Med Ctr, Peoples Hosp, Beijing 100871, Peoples R China
5.Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biothechnol, Lab Chem Genom, Shenzhen, Peoples R China
6.Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
推荐引用方式
GB/T 7714
Yang, Weifeng,Wei, Wei,Shi, Cheng,et al. Pluripotin Combined with Leukemia Inhibitory Factor Greatly Promotes the Derivation of Embryonic Stem Cell Lines from Refractory Strains[J]. STEM CELLS,2009,27(2):383-389.
APA Yang, Weifeng.,Wei, Wei.,Shi, Cheng.,Zhu, Jinliang.,Ying, Wenqin.,...&Deng, Hongkui.(2009).Pluripotin Combined with Leukemia Inhibitory Factor Greatly Promotes the Derivation of Embryonic Stem Cell Lines from Refractory Strains.STEM CELLS,27(2),383-389.
MLA Yang, Weifeng,et al."Pluripotin Combined with Leukemia Inhibitory Factor Greatly Promotes the Derivation of Embryonic Stem Cell Lines from Refractory Strains".STEM CELLS 27.2(2009):383-389.
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