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NF-kappa Bp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1 beta-induced and TNF-alpha-induced chondrocytes
Lianxu, C; Hongti, J; Changlong, Y
关键词Sirna Nf-kappa Bp65 Articular Chondrocytes Cox-2 Nos-2 Mmp-9 Il-1 Beta Tnf-alpha
刊名OSTEOARTHRITIS AND CARTILAGE
2006-04-01
DOI10.1016/j.joca.2005.10.009
14期:4页:367-376
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Orthopedics ; Rheumatology
研究领域[WOS]Orthopedics ; Rheumatology
关键词[WOS]NF-KAPPA-B ; NITRIC-OXIDE SYNTHASE ; DOUBLE-STRANDED-RNA ; TRANSCRIPTION FACTOR ; EXPERIMENTAL OSTEOARTHRITIS ; INFLAMMATORY DISEASES ; MESSENGER-RNA ; CARTILAGE ; INTERFERENCE ; ACTIVATION
英文摘要

Objective: Small interfering RNA (siRNA) triggers RNA interference in mammalian somatic cells. Nuclear factor kappa B (NF-kappa B) is a transcription factor that is implicated in inflammation and immune activation. This study was to use NF-kappa Bp65-specific siRNA to inhibit the expression of genes of cyclooxygenase-2 (COX-2), nitric oxide synthase-2 (NOS-2) and matrix metalloproteinase-9 (MMP-9), which is paralleled with the initiation and progression of cartilage lesions in osteoarthritis (OA) model, in induced chondrocytes, and therefore to explore a new gene therapy for OA.

Methods: Western blot and reverse transcriptase polymerase chain reaction (RT-PCR) were performed to optimize the silencing effects of NF-kappa Bp65-specific siRNA in cultured rat chondrocytes, and then to determine the expression of COX-2, NOS-2 and MMP-9 in induced chondrocytes. The activation of NF-kappa B was determined by electrophoretic mobility shift assay (EMSA). Western blot and RT-PCR were subjected to densitometric analysis and then band intensities were also determined.

Results: The NF-kappa Bp65-specific siRNA inhibited the expression of NF-kappa Bp65 and activation of NF-kappa B, reducing significantly the expression of COX-2, NOS-2 and MMP-9 induced by interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) in cultured chondrocytes.

Conclusions: NF-kappa Bp65-specific siRNA can inhibit the expression of COX-2, NOS-2 and MMP-9 in IL-1 beta-induced and TNF-alpha-induced chondrocytes. This suggests that NF-kappa Bp65-specific siRNA has potential to be a useful, preventive and therapeutic agent for OA at early stages. (c) 2005 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000237917900007
引用统计
被引频次:94[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51213
专题北京大学第三临床医学院_运动医学研究所
作者单位Peking Univ, Hosp 3, Inst Sports Med, Beijing 100083, Peoples R China
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GB/T 7714
Lianxu, C,Hongti, J,Changlong, Y. NF-kappa Bp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1 beta-induced and TNF-alpha-induced chondrocytes[J]. OSTEOARTHRITIS AND CARTILAGE,2006,14(4):367-376.
APA Lianxu, C,Hongti, J,&Changlong, Y.(2006).NF-kappa Bp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1 beta-induced and TNF-alpha-induced chondrocytes.OSTEOARTHRITIS AND CARTILAGE,14(4),367-376.
MLA Lianxu, C,et al."NF-kappa Bp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1 beta-induced and TNF-alpha-induced chondrocytes".OSTEOARTHRITIS AND CARTILAGE 14.4(2006):367-376.
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