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学科主题: 临床医学
题名:
Combination of Targeted PDT and Anti-VEGF Therapy for Rat CNV by RGD-Modified Liposomal Photocyanine and Sorafenib
作者: Wang, Jia-lin1; Xi, Yi1; Liu, Yu-ling1; Wang, Zhao-hui2; Zhang, Qiang2
关键词: choroidal neovascularization ; targeted PDT ; anti-VEGF ; photocyanine ; sorafenib ; RGD peptide ; liposomes
刊名: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
发表日期: 2013-12-01
DOI: 10.1167/iovs.13-13068
卷: 54, 期:13, 页:7983-7989
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: EXPERIMENTAL CHOROIDAL NEOVASCULARIZATION ; PHOTODYNAMIC THERAPY ; MACULAR DEGENERATION ; COMBRETASTATIN A-4 ; CELLS ; DELIVERY ; RANIBIZUMAB ; DOXORUBICIN ; APOPTOSIS ; ZNPCS2P2
英文摘要:

PURPOSE. To achieve a combination therapy of targeted PDT and anti-VEGF for choroidal neovascularization (CNV).

METHODS. Arg-Gly-Asp (RGD)-modified liposomes encapsulating photocyanine and sorafenib (RGD-SSL-[P]-[S]) were prepared and characterized. Drug concentration in RGD-SSL-[P]-[S] and irradiation time were optimized on ARPE-19 and HUVEC cells in vitro. A laser-induced CNV rat model was used to assess the efficacy of this targeted combinational system. The effect of RGD-SSL-[P]-[S] on the retinal structure of BN rats was also examined.

RESULTS. The particle size of RGD-SSL-[P]-[S] was approximately 100 nm, with a encapsulation efficiency more than 90% for both photocyanine and sorafenib. From RGD-SSL-[P]-[S], the release rate of photocyanine was approximately 22%, whereas that of sorafeinb was approximately 40% at 48 hours. With the optimal drug concentration and irradiation time, RGD-SSL-[P]-[S] exhibited cytotoxicity only to HUVEC without obvious damage to normal ARPE-19 cells. Rats treated with RGD-SSL-[P]-[S] showed the least CNV area and fluorescein leakage in fluorescein fundus angiography. RGD-SSL-[P]-[S] was also found safe to the rat retina.

CONCLUSIONS. Combination of targeted PDT and anti-VEGF might be an effective therapy for CNV.

语种: 英语
所属项目编号: 81130059 ; 2009CB930300 ; 2009ZX09310-001 ; BMU20110263
项目资助者: National Science Foundation ; Ministry of Science and Technology of China ; Ministry of Education
WOS记录号: WOS:000328133800019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51223
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Ctr Eye, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimot Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Jia-lin,Xi, Yi,Liu, Yu-ling,et al. Combination of Targeted PDT and Anti-VEGF Therapy for Rat CNV by RGD-Modified Liposomal Photocyanine and Sorafenib[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2013,54(13):7983-7989.
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