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学科主题临床医学
Differences in biological features of gastric dysplasia, indefinite dysplasia, reactive hyperplasia and discriminant analysis of these lesions
Dong, Bin1; Xie, Yu-Quan1; Chen, Ke1; Wang, Tao2; Tang, Wei1; You, Wei-Cheng1; Li, Ji-You1
关键词Gastric Dysplasia Indefinite Dysplasia Reactive Hyperplasia
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2005-06-21
11期:23页:3595-3600
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
资助者National Key Fundamental Research Project ; National Key Fundamental Research Project
研究领域[WOS]Gastroenterology & Hepatology
英文摘要

AIM: To investigate the differences in biological features of gastric dysplasia (Dys), indefinite dysplasia (IDys) and reactive hyperplasia ( RH) by studying the biomarker alterations in cell proliferation, cell differentiation, cell cycle control and the expression of house-keeping genes, and further to search for markers which could be used in guiding the pathological diagnosis of three lesions.

METHODS: Expressions of MUC5AC, MUC6, adenomatous polyposis coli (APC), p53, Ki-67, proliferation cell nuclear antigen (PCNA) and EGFR were studied by immunohistochemistry with a standard Envision technique in formalin-fixed and paraffin-embedded specimens from 43 RH, 35 IDys, 35 Dys and 36 intestinal type gastric carcinomas (IGC). In addition, Bayes discriminant analysis was used to investigate the value of markers studied in differential diagnosis of RH, IDys, Dys and IGC. RESULTS: The MUC5AC and MUC6 antigen expressions in RH, IDys, Dys and IGC decreased gradually (MUC5AC: 86.04%, 77.14%, 28.57%, 6.67%; MUC6: 65.15%, 54.29%, 20.00%, 25.00%, respectively). The expressions of the two markers had no significant difference between RH and IDys, but were all significantly higher than those of the other two lesions (MUC5AC: chi(2) = 27.607, 38.027 and 17.33, 26.092; MUC6: chi(2) = 16.54, 12.665 and 9.282, 6.737, P<0.01). There was no significant difference between RH and IDys, Dys and IGC in MUC6 expression. The APC gene expression in the four lesions had a similar decreasing tendency (RH 69.76%, IDys 68.57%, Dys 39.39%, IGC 22.86%), and it was significantly higher in the first two lesions than in the last two (chi(2) = 7.011, 16.995 and 14.737, 19.817, P<0.05). The p53 expression in RH, IDys, Dys and IGC was 6.98%, 20%, 57.14% and 50%, respectively. There was no significant difference between RH and IDys or Dys and IGC, but the p53 expression in RH and IDys was significantly lower than that in Dys and IGC (chi(2) = 7.011, 16.995 and 14.737, 19.817, P<0.01). The Ki-67 label index was significantly different among four lesions (RH:0.298 +/- 8.92%, IDys: 0.358 +/- 9.25%, Dys: 0.498 +/- 9.03%, IGC: 0.620 +/- 10.8%, P<0.001). Positive immunostaining of PCNA was though observed in all specimens, significant differences were detected among four lesions (F = 95.318, P<0.01). In addition, we used Bayes discriminant analysis to investigate molecular pathological classification of the lesions, and obtained the best result with the combination of MUC5AC, Ki-67 and PCNA. The overall rate of correct classification was 67.4% (RH), 68.6% (IDys), 70.6% (Dys) and 84.8% (IGC), respectively.

CONCLUSION: Dys has neoplastic biological characteristics, while RH and IDys display hyperplastic characteristics. MUC5AC and proliferation-related biomarkers (Ki-67, PCNA) are more specific in distinguishing Dys from RH and IDys. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

语种英语
所属项目编号G1998051203
资助者National Key Fundamental Research Project ; National Key Fundamental Research Project
WOS记录号WOS:000208099000023
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51242
Collection北京大学临床肿瘤学院_病理科
作者单位1.Peking Univ, Dept Pathol, Sch Oncol, Beijing Canc Hosp,Beijing Inst Canc Res, Beijing 100036, Peoples R China
2.Peking Univ, Sch Publ Hlth, Dept Epidemiol, Beijing 100085, Peoples R China
Recommended Citation
GB/T 7714
Dong, Bin,Xie, Yu-Quan,Chen, Ke,et al. Differences in biological features of gastric dysplasia, indefinite dysplasia, reactive hyperplasia and discriminant analysis of these lesions[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(23):3595-3600.
APA Dong, Bin.,Xie, Yu-Quan.,Chen, Ke.,Wang, Tao.,Tang, Wei.,...&Li, Ji-You.(2005).Differences in biological features of gastric dysplasia, indefinite dysplasia, reactive hyperplasia and discriminant analysis of these lesions.WORLD JOURNAL OF GASTROENTEROLOGY,11(23),3595-3600.
MLA Dong, Bin,et al."Differences in biological features of gastric dysplasia, indefinite dysplasia, reactive hyperplasia and discriminant analysis of these lesions".WORLD JOURNAL OF GASTROENTEROLOGY 11.23(2005):3595-3600.
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