|Renal cell carcinoma related novel gene, GYLZ-RCC18: cloning and functional studies|
|Zhang, Q1; Chen, L1; Liang, LL1; Xi, ZJ1; Ding, Y1; Tong, M1; Zhang, ZW1; Lee, C1; Guo, YL1|
|关键词||Kidney Neoplasm Carcinoma Gene Gylz-rcc18|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
Objective To clone the full length of renal cell carcinoma (RCC) related novel gene GYLZ-RCC18 and study its function.
Methods SMART RACE technology was used to clone the full length of GYLZ-RCC18. RT-PCR was used to detect its expression in renal cell carcinoma tissue at different stages and grades. We transfected the antisense oligonucleotide of GYLZ-RCC18 to renal cell carcinoma cell line, GRC-1, and analyzed proliferation activity, growth rate, apoptosis, and mortality changes.
Results The full length of GYLZ-RCC18 (GenBank accession number: BE825133) cDNA was about 3.5 kb. GYLZ-RCC18 had a higher expression in higher grades and stages of renal cell carcinoma than in lower ones. The expression of GYLZ-RCC18 in renal cell carcinoma was much higher than in normal kidney. After the transfection of GYLZ-RCC18 antisense oligonucleoticle, the mortality of GRC-1 increased significantly, while proliferative activity and growth rate were substantially inhibited at the same time. The antisense oligonucleotide induced apoptosis of GRC-1 through the entire observation time.
Conclusion GYLZ-RCC18 is an important novel gene related to renal cell carcinoma. Overexpression of this gene results in higher growth and proliferative activity and has an antiapoptosis effect on renal cell carcinoma cells. Transfection of the antisense oligonucleotide may inhibit the generation and development of renal cell carcinoma.
|作者单位||1.Peking Univ, Hosp 1, Inst Urol, Beijing 100034, Peoples R China|
2.Northwestern Univ, Sch Med, Evanston, IL 60208 USA
3.Peking Univ, Affiliated Hosp 1, Dept Urol, Beijing 100034, Peoples R China
4.Peking Univ, Affiliated Hosp 1, Inst Urol, Beijing 100034, Peoples R China
5.Fujian Med Univ, Inst Hematol, Xiamen 361001, Peoples R China
|Zhang, Q,Chen, L,Liang, LL,et al. Renal cell carcinoma related novel gene, GYLZ-RCC18: cloning and functional studies[J]. CHINESE MEDICAL JOURNAL,2002,115(5):746-749.|
|APA||Zhang, Q.,Chen, L.,Liang, LL.,Xi, ZJ.,Ding, Y.,...&Guo, YL.(2002).Renal cell carcinoma related novel gene, GYLZ-RCC18: cloning and functional studies.CHINESE MEDICAL JOURNAL,115(5),746-749.|
|MLA||Zhang, Q,et al."Renal cell carcinoma related novel gene, GYLZ-RCC18: cloning and functional studies".CHINESE MEDICAL JOURNAL 115.5(2002):746-749.|