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学科主题: 临床医学
题名:
Screening for genes associated with ovarian cancer prognosis
作者: Chang Xiao-hong1; Zhang Li2; Yang Rong1; Feng Jie1; Cheng Ye-xia1; Cheng Hong-yan1; Ye Xue1; Fu Tian-yun1; Cui Heng1
关键词: ovarian cancer ; microarray ; cell receptor ; immunity-associated ; prognosis
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2009-05-20
DOI: 10.3760/cma.j.issn.0366-6999.2009.10.010
卷: 122, 期:10, 页:1167-1172
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: EXPRESSION ; MICROARRAY ; PCM-1
英文摘要:

Background Human epithelial ovarian cancer cell line SKOV3.ip1 is more invasive and metastatic compared with its parental line SKOV3. A total of 17 000 human genome complementary DNA microarrays were used to compare the gene expression patterns of the two cell lines. Based on this, the gene expression profiles of 22 patients with ovarian cancer were analyzed by cDNA microarray, and screened the 2-fold differentially expressed genes compared with the normal ones. We screened genes relevant to clinical prognosis of serous ovarian cancer by determining the expression profiles of ovarian cancer genes to investigate cell receptor and immunity-associated genes, and as groundwork, identify ovarian cancer-associated antigens at the gene level.

Methods Total RNA was extracted from 22 patients with ovarian cancer and DNA microarrays were prepared. After scanning, hybridization signals were collected and the genes that were differentially expressed twice as compared with the normal ones were screened.

Results We screened 236 genes relevant to the prognosis of ovarian cancer from the 17 000 human genome cDNA microarrays. According to gene classification, 48 of the 236 genes were cell receptor or immunity-associated genes, including 2 genes related to the International Federation of Gynecology and Obstetrics (FIGO) stage, 4 genes to histological grade, 18 genes to lymph node metastasis, 11 genes to residual disease, and 13 genes to the reactivity to chemotherapy. Several functionally important genes including fibronectin 1, pericentriolar material 1, beta-2-microglobulin, PPAR binding protein were identified through review of the literature.

Conclusions The cDNA microarray of ovarian cancer genes developed in this study was effective and high throughput in screening the ovarian cancer-associated genes differentially expressed. Through the studies of the cell receptor and immunity-associated genes we expect to identify the molecular biology index of ovarian cancer-associated antigens. Chin Med J 2009;122(10):1167-1172

语种: 英语
WOS记录号: WOS:000266383000010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51266
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

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作者单位: 1.Shangdi Hosp, Dept Obstet, Beijing 100084, Peoples R China
2.Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Chang Xiao-hong,Zhang Li,Yang Rong,et al. Screening for genes associated with ovarian cancer prognosis[J]. CHINESE MEDICAL JOURNAL,2009,122(10):1167-1172.
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