IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
Insufficient Radiofrequency Ablation Promotes Angiogenesis of Residual Hepatocellular Carcinoma via HIF-1 alpha/VEGFA
Kong, Jian1; Kong, Jinge2,3,4; Pan, Bing2,3,4; Ke, Shan1; Dong, Shuying1; Li, Xiuli5; Zhou, Aimin6; Zheng, Lemin2,3,4; Sun, Wen-bing1
刊名PLOS ONE
2012-05-15
DOI10.1371/journal.pone.0037266
7期:5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; "973" National S&amp ; T Major Project ; Natural Science Foundation of Beijing ; Natural Science Foundation of Fujian ; National Natural Science Foundation of China ; "973" National S&amp ; T Major Project ; Natural Science Foundation of Beijing ; Natural Science Foundation of Fujian
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; COLORECTAL LIVER METASTASES ; INDUCIBLE FACTOR 1-ALPHA ; SQUAMOUS-CELL CARCINOMA ; TUMOR ANGIOGENESIS ; RAPID PROGRESSION ; THERMAL ABLATION ; VEGF EXPRESSION ; KEY MEDIATOR ; HYPOXIA
英文摘要

Background: The mechanism of rapid growth of the residual tumor after radiofrequency (RF) ablation is poorly understood. In this study, we investigated the effect of hyperthermia on HepG2 cells and generated a subline with enhanced viability and dys-regulated angiogenesis in vivo, which was used as a model to further determine the molecular mechanism of the rapid growth of residual HCC after RF ablation.

Methodology/Principal Findings: Heat treatment was used to establish sublines of HepG2 cells. A subline (HepG2 k) with a relatively higher viability and significant heat tolerance was selected. The cellular protein levels of VEGFA, HIF-1 alpha and p-Akt, VEGFA mRNA and secreted VEGFA were measured, and all of these were up-regulated in this subline compared to parental HepG2 cells. HIF-1 alpha inhibitor YC-1 and VEGFA siRNA inhibited the high viability of the subline. The conditioned media from the subline exerted stronger pro-angiogenic effects. Bevacizumab, VEGFA siRNA and YC-1 inhibited proangiogenic effects of the conditioned media of HepG2 k cells and abolished the difference between parental HepG2 cells and HepG2 k cells. For in vivo studies, a nude mouse model was used, and the efficacy of bavacizumab was determined. HepG2 k tumor had stronger pro-angiogenic effects than parental HepG2 tumor. Bevacizumab could inhibit the tumor growth and angiogenesis, and also eliminate the difference in tumor growth and angiogenesis between parental HepG2 tumor and HepG2 k tumor in vivo.

Conclusions/Significance: The angiogenesis induced by HIF1 alpha/VEGFA produced by altered cells after hyperthermia treatment may play an important role in the rapid growth of residual HCC after RF ablation. Bevacizumab may be a good candidate drug for preventing and treating the process.

语种英语
所属项目编号30872490 ; 30900595 ; 81172500 ; 81170101 ; 2010CB912504 ; 2011CB503900 ; 7102104 ; 2009J01112
资助者National Natural Science Foundation of China ; "973" National S&amp ; T Major Project ; Natural Science Foundation of Beijing ; Natural Science Foundation of Fujian ; National Natural Science Foundation of China ; "973" National S&amp ; T Major Project ; Natural Science Foundation of Beijing ; Natural Science Foundation of Fujian
WOS记录号WOS:000305336300087
引用统计
被引频次:46[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51277
专题北京大学基础医学院_心血管所
作者单位1.Capital Med Univ, Beijing Chao Yang Hosp, Dept Hepatobiliary Surg, Beijing, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Minist Educ, Inst Cardiovasc Sci, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ, Inst Syst Biomed,Sch Basic Med Sci, Beijing 100871, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100871, Peoples R China
5.Chinese Peoples Liberat Army Gen Hosp, Dept Gynecol & Obstet, Beijing, Peoples R China
6.Cleveland State Univ, Dept Chem, Clin Chem Program, Cleveland, OH 44115 USA
推荐引用方式
GB/T 7714
Kong, Jian,Kong, Jinge,Pan, Bing,et al. Insufficient Radiofrequency Ablation Promotes Angiogenesis of Residual Hepatocellular Carcinoma via HIF-1 alpha/VEGFA[J]. PLOS ONE,2012,7(5).
APA Kong, Jian.,Kong, Jinge.,Pan, Bing.,Ke, Shan.,Dong, Shuying.,...&Sun, Wen-bing.(2012).Insufficient Radiofrequency Ablation Promotes Angiogenesis of Residual Hepatocellular Carcinoma via HIF-1 alpha/VEGFA.PLOS ONE,7(5).
MLA Kong, Jian,et al."Insufficient Radiofrequency Ablation Promotes Angiogenesis of Residual Hepatocellular Carcinoma via HIF-1 alpha/VEGFA".PLOS ONE 7.5(2012).
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
journal.pone.0037266(2259KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Kong, Jian]的文章
[Kong, Jinge]的文章
[Pan, Bing]的文章
百度学术
百度学术中相似的文章
[Kong, Jian]的文章
[Kong, Jinge]的文章
[Pan, Bing]的文章
必应学术
必应学术中相似的文章
[Kong, Jian]的文章
[Kong, Jinge]的文章
[Pan, Bing]的文章
相关权益政策
暂无数据
收藏/分享
文件名: journal.pone.0037266.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。