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学科主题: 临床医学
题名:
Serum IgA against type 3 muscarinic acetylcholine receptor is a novel marker in diagnosis of Sjogren′s syndrome
作者: Li Ying-ni1; Guo Jian-ping1; He Jing1; Liu Xia2; Yin Fang-rui3; Ding Yan1; Yao Hai-hong1; Kang Ai-jun4; Li Zhan-guo1
关键词: Sjogren&prime ; s syndrome ; autoantibodies ; type 3 muscarinic acetylcholine receptor ; enzyme-linked immunosorbent assay
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2011-08-20
DOI: 10.3760/cma.j.issn.0366-6999.2011.16.017
卷: 124, 期:16, 页:2490-2495
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: REVISED CRITERIA ; AUTOANTIBODIES ; ANTIBODIES ; CLASSIFICATION ; PATHOGENESIS ; PEPTIDE
英文摘要:

Background Antibodies against type 3 muscarinic acetylcholine receptor (M3R) are involved in the pathogenesis of Sjogren′s syndrome (SS), but the clinical value of them in SS patients has been controversial. The aims of this study were to: (1) establish an improved enzyme-linked immunosorbent assay (ELISA) to detect IgA antibodies against M3R; (2) evaluate the value of IgA antibodies against the second extracellular loop of M3R205-220 (c2M3RP) in diagnosis of SS.

Methods To increase the ELISA sensitivity, c2M3RP was coupled to bovine serum albumin (BSA) by the glutaraldehyde method and a 96-well microplate was treated by ultraviolet rays before coated. Concentrations of anti-c2M3RP, anti-SSA, and anti-SSB were measured in the sera of 240 individuals: 91 patients with primary SS and 149 controls (16 secondary SS, 27 systemic lupus erythematosus, 40 rheumatoid arthritis and 66 healthy controls). Diagnostic properties of anti-c2M3RP were determined by receiver-operating characteristic curve analysis.

Results The prevalence of serum IgA anti-c2M3RP antibodies in patients with pSS (46%, 42/91) was significantly higher than that in patients with systemic lupus erythematosus (19%, 5/27), in rheumatoid arthritis (15%, 6/40) and in healthy controls (5%, 3/66). However, there was no significant difference between the two SS groups (P=0.727). The diagnostic performance of IgA anti-M3RP antibodies was similar to anti-SSA assay, but had 22% higher sensitivity than anti-SSB. By analyzing of IgA anti-c2M3RP antibodies, combination of anti-SSA and anti-SSB resulted in increased sensitivity, whereas their specificity was not significantly changed.

Conclusions The improved anti-c2M3RP ELISA is a novel, sensitive, and specific serological test for the diagnosis of SS. The combined application of anti-c2M3RP, anti-SSA and anti-SSB tests can improve the laboratory diagnosis of SS. The IgA anti-c2M3RP antibodies may serve as a novel diagnostic marker for SS. Chin Med J 2011;124(16):2490-2495

语种: 英语
所属项目编号: 31070788
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000294584200017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51292
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100044, Peoples R China
2.China Japan Friendship Hosp, Dept Rheumatol & Immunol, Beijing 100029, Peoples R China
3.Baotou Med Coll, Affiliated Hosp 1, Dept Rheumatol & Immunol, Baotou 014010, Inner Mongolia, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Lab Anim Sci, Beijing 100191, Peoples R China

Recommended Citation:
Li Ying-ni,Guo Jian-ping,He Jing,et al. Serum IgA against type 3 muscarinic acetylcholine receptor is a novel marker in diagnosis of Sjogren′s syndrome[J]. CHINESE MEDICAL JOURNAL,2011,124(16):2490-2495.
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