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The Expression Levels of Transcription Factors T-bet, GATA-3, ROR gamma t and FOXP3 in Peripheral Blood Lymphocyte (PBL) of Patients with Liver Cancer and their Significance
Lin, Ze-Wei1; Wu, Li-Xuan2; Xie, Yong1; Ou, Xi1; Tian, Pei-Kai1; Liu, Xiao-Ping1; Min, Jun3; Wang, Jie3; Chen, Ru-Fu3; Chen, Ya-Jing3; Liu, Chao3; Ye, Hua3; Ou, Qing-Jia3
关键词Ror Gamma t Th1 Th2 Th17 Treg Hepatocellular Carcinoma (Hcc)
刊名INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
2015
DOI10.7150/ijms.8352
12期:1页:7-16
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
资助者Shenzhen Municipal Science and Technology Planning Projects ; Shenzhen Municipal Science and Technology Planning Projects
研究领域[WOS]General & Internal Medicine
关键词[WOS]HEPATOCELLULAR-CARCINOMA ; CELL-DIFFERENTIATION ; CYTOKINE PROFILE ; DENDRITIC CELLS ; BREAST-CANCER ; UPDATED VIEW ; TGF-BETA ; BALANCE ; VACCINATION ; RESPONSES
英文摘要

Objectives: To investigate the expression of transcriptional factors (TFs) T-bet, GATA-3, ROR gamma t and FOXP in peripheral blood mononuclear cells (PBMC) of patients with hepatocellular carcinoma (HCC) and to evaluate the correlation between the imbalances of Th1/Th2, Th17/Treg at the expression levels and liver cancer

Methods: The peripheral venous blood was drawn from 20 HCC-patients (HCC-group) and 20 health participants (C-group). The expression levels of Th1, Th2 and Th17 and the major Treg-specific TFs T-bet, GATA-3, ROR.t and FOXP3 in the PBMC were measured with quantitative real-time PCR(RT-qPCR).

Results: The mRNA level of Th1-specific TF T-bet in HCC-group was significantly lower than that of C-group (52.34 +/- 34.07 VS 104.01 +/- 56.00, P<0.01); the mRNA level of Th2-specifc TF, GATA-3, in HCC group was significantly higher than that in C-group (1.38 +/- 1.15 VS 0.58 +/- 0.65, P<0.05) and T-bet mRNA/GATA-3 mRNA ratio was significantly lower in HCC-group than in C-group (86.01 +/- 116.71 VS 461.88 +/- 708.81, P<0.05). The mRNA level of Th17-specific TF ROR.t in HCC-group was significantly higher than that of C-group (72.32 +/- 32.82 VS 33.07 +/- 22.86, P<0.01). Treg-specific TF FOXP3 mRNA level was significant higher in HCC-group than in C-group (3.17 +/- 1.59 VS 1.39 +/- 1.13, P<0.01)

Conclusion: T-bet mRNA level was reduced whereas GATA-3 mRNA level was increased and T-bet/GATA-3 ratio was significantly reduced in PBMC, indicating that Th1/Th2 ratio was of imbalance at TF levels in PBMC of HCC, displaying Th2 thrift phenomena. The mRNA levels of ROR.t and FOXP3 in PBMC of HCC were significantly increased, indicating the existence of a predominant phenomenon of Th17- and Treg-expressing PBMC in HCC.

语种英语
所属项目编号JC200903180670A ; 201103023
资助者Shenzhen Municipal Science and Technology Planning Projects ; Shenzhen Municipal Science and Technology Planning Projects
WOS记录号WOS:000351944800002
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51307
专题北京大学深圳医院
作者单位1.Peking Univ, Shenzhen Hosp, Dept Hepatobiliary Laparoscope Surg, Shenzhen 518036, Guangdong, Peoples R China
2.Cent People Hosp Huizhou City, Dept Hepatobiliary Surg, Huizhou 516001, Guangdong, Peoples R China
3.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Hepatobiliary Surg, Guangzhou 510120, Guangdong, Peoples R China
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Lin, Ze-Wei,Wu, Li-Xuan,Xie, Yong,et al. The Expression Levels of Transcription Factors T-bet, GATA-3, ROR gamma t and FOXP3 in Peripheral Blood Lymphocyte (PBL) of Patients with Liver Cancer and their Significance[J]. INTERNATIONAL JOURNAL OF MEDICAL SCIENCES,2015,12(1):7-16.
APA Lin, Ze-Wei.,Wu, Li-Xuan.,Xie, Yong.,Ou, Xi.,Tian, Pei-Kai.,...&Ou, Qing-Jia.(2015).The Expression Levels of Transcription Factors T-bet, GATA-3, ROR gamma t and FOXP3 in Peripheral Blood Lymphocyte (PBL) of Patients with Liver Cancer and their Significance.INTERNATIONAL JOURNAL OF MEDICAL SCIENCES,12(1),7-16.
MLA Lin, Ze-Wei,et al."The Expression Levels of Transcription Factors T-bet, GATA-3, ROR gamma t and FOXP3 in Peripheral Blood Lymphocyte (PBL) of Patients with Liver Cancer and their Significance".INTERNATIONAL JOURNAL OF MEDICAL SCIENCES 12.1(2015):7-16.
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