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学科主题: 临床医学
题名:
The Expression Levels of Transcription Factors T-bet, GATA-3, ROR gamma t and FOXP3 in Peripheral Blood Lymphocyte (PBL) of Patients with Liver Cancer and their Significance
作者: Lin, Ze-Wei1; Wu, Li-Xuan2; Xie, Yong1; Ou, Xi1; Tian, Pei-Kai1; Liu, Xiao-Ping1; Min, Jun3; Wang, Jie3; Chen, Ru-Fu3; Chen, Ya-Jing3; Liu, Chao3; Ye, Hua3; Ou, Qing-Jia3
关键词: ROR gamma t ; Th1 ; Th2 ; Th17 ; Treg ; Hepatocellular carcinoma (HCC)
刊名: INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
发表日期: 2015
DOI: 10.7150/ijms.8352
卷: 12, 期:1, 页:7-16
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ; CELL-DIFFERENTIATION ; CYTOKINE PROFILE ; DENDRITIC CELLS ; BREAST-CANCER ; UPDATED VIEW ; TGF-BETA ; BALANCE ; VACCINATION ; RESPONSES
英文摘要:

Objectives: To investigate the expression of transcriptional factors (TFs) T-bet, GATA-3, ROR gamma t and FOXP in peripheral blood mononuclear cells (PBMC) of patients with hepatocellular carcinoma (HCC) and to evaluate the correlation between the imbalances of Th1/Th2, Th17/Treg at the expression levels and liver cancer

Methods: The peripheral venous blood was drawn from 20 HCC-patients (HCC-group) and 20 health participants (C-group). The expression levels of Th1, Th2 and Th17 and the major Treg-specific TFs T-bet, GATA-3, ROR.t and FOXP3 in the PBMC were measured with quantitative real-time PCR(RT-qPCR).

Results: The mRNA level of Th1-specific TF T-bet in HCC-group was significantly lower than that of C-group (52.34 +/- 34.07 VS 104.01 +/- 56.00, P<0.01); the mRNA level of Th2-specifc TF, GATA-3, in HCC group was significantly higher than that in C-group (1.38 +/- 1.15 VS 0.58 +/- 0.65, P<0.05) and T-bet mRNA/GATA-3 mRNA ratio was significantly lower in HCC-group than in C-group (86.01 +/- 116.71 VS 461.88 +/- 708.81, P<0.05). The mRNA level of Th17-specific TF ROR.t in HCC-group was significantly higher than that of C-group (72.32 +/- 32.82 VS 33.07 +/- 22.86, P<0.01). Treg-specific TF FOXP3 mRNA level was significant higher in HCC-group than in C-group (3.17 +/- 1.59 VS 1.39 +/- 1.13, P<0.01)

Conclusion: T-bet mRNA level was reduced whereas GATA-3 mRNA level was increased and T-bet/GATA-3 ratio was significantly reduced in PBMC, indicating that Th1/Th2 ratio was of imbalance at TF levels in PBMC of HCC, displaying Th2 thrift phenomena. The mRNA levels of ROR.t and FOXP3 in PBMC of HCC were significantly increased, indicating the existence of a predominant phenomenon of Th17- and Treg-expressing PBMC in HCC.

语种: 英语
所属项目编号: JC200903180670A ; 201103023
项目资助者: Shenzhen Municipal Science and Technology Planning Projects
WOS记录号: WOS:000351944800002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51307
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Dept Hepatobiliary Laparoscope Surg, Shenzhen 518036, Guangdong, Peoples R China
2.Cent People Hosp Huizhou City, Dept Hepatobiliary Surg, Huizhou 516001, Guangdong, Peoples R China
3.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Hepatobiliary Surg, Guangzhou 510120, Guangdong, Peoples R China

Recommended Citation:
Lin, Ze-Wei,Wu, Li-Xuan,Xie, Yong,et al. The Expression Levels of Transcription Factors T-bet, GATA-3, ROR gamma t and FOXP3 in Peripheral Blood Lymphocyte (PBL) of Patients with Liver Cancer and their Significance[J]. INTERNATIONAL JOURNAL OF MEDICAL SCIENCES,2015,12(1):7-16.
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