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学科主题: 临床医学
题名:
Natural autoantibodies to myeloperoxidase, proteinase 3, and the glomerular basement membrane are present in normal individuals
作者: Cui, Zhao2; Zhao, Ming-hui2; Segelmark, Marten1; Hellmark, Thomas1
关键词: Goodpasture&prime ; s disease ; myeloperoxidase ; natural autoantibodies ; proteinase 3 ; vasculitis
刊名: KIDNEY INTERNATIONAL
发表日期: 2010-09-01
DOI: 10.1038/ki.2010.198
卷: 78, 期:6, 页:590-597
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: NORMAL HUMAN SERA ; WEGENERS-GRANULOMATOSIS ; MICROSCOPIC POLYANGIITIS ; T-CELLS ; ANTIMYELOPEROXIDASE AUTOANTIBODIES ; AUTOIMMUNE GLOMERULONEPHRITIS ; CRESCENTIC GLOMERULONEPHRITIS ; CYTOPLASMIC AUTOANTIBODIES ; GOODPASTURE AUTOANTIGEN ; CRYPTIC EPITOPES
英文摘要:

Anti-neutrophil cytoplasmic antibodies (ANCAs) have a pathogenic role in ANCA-associated vasculitis. The origin of ANCAs and anti-glomerular basement membrane (GBM) antibodies, however, is unknown. In this study, we determined whether natural autoantibodies against myeloperoxidase (MPO), proteinase 3 (PR3), and GBM were present in each of 10 healthy Chinese and Swedish individuals, negative for all three antigens by routine ELISA. Antibodies were purified from isolated IgG by antigen-specific affinity columns. Natural anti-GBM autoantibodies gave a linear staining pattern along the GBM of human renal sections. On ethanol-fixed granulocytes, both natural anti-MPO and anti-PR3 autoantibodies gave cytoplasmic staining. The titers of natural anti-MPO/PR3 autoantibodies were significantly lower than those from patients with vasculitis. In competition ELISA, the binding of natural anti-MPO autoantibodies could be inhibited by MPO, but not by PR3 or noncollagenous domains from type IV collagen. The same specificity results were found for natural anti-PR3 and anti-GBM autoantibodies. Overall, individuals of the Chinese origin had more natural autoantibodies than did those of the Swedish origin, but no other differences were found. Hence, our study shows that healthy individuals have masked circulating, noncross-reactive, antigen-specific natural autoantibodies against MPO, PR3, and GBM in their serum and IgG fractions. Further studies are needed to determine their role if any in the etiology of ANCA-associated vasculitis and anti-GBM disease. Kidney International (2010) 78, 590-597; doi:10.1038/ki.2010.198; published online 30 June 2010

语种: 英语
所属项目编号: 30725034 ; 30700752 ; 71X-15152
项目资助者: National Natural Science Fund of China ; Swedish Research Council ; Lund University Hospital ; Crafoord Foundation
WOS记录号: WOS:000281467100011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51308
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: 1.Lund Univ, Dept Nephrol, SE-22184 Lund, Sweden
2.Peking Univ, Inst Nephrol, Hosp 1, Renal Div,Dept Med, Beijing 100871, Peoples R China

Recommended Citation:
Cui, Zhao,Zhao, Ming-hui,Segelmark, Marten,et al. Natural autoantibodies to myeloperoxidase, proteinase 3, and the glomerular basement membrane are present in normal individuals[J]. KIDNEY INTERNATIONAL,2010,78(6):590-597.
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