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学科主题临床医学
Natural autoantibodies to myeloperoxidase, proteinase 3, and the glomerular basement membrane are present in normal individuals
Cui, Zhao2; Zhao, Ming-hui2; Segelmark, Marten1; Hellmark, Thomas1
关键词Goodpasture&Prime s Disease Myeloperoxidase Natural Autoantibodies Proteinase 3 Vasculitis
刊名KIDNEY INTERNATIONAL
2010-09-01
DOI10.1038/ki.2010.198
78期:6页:590-597
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
资助者National Natural Science Fund of China ; Swedish Research Council ; Lund University Hospital ; Crafoord Foundation ; National Natural Science Fund of China ; Swedish Research Council ; Lund University Hospital ; Crafoord Foundation
研究领域[WOS]Urology & Nephrology
关键词[WOS]NORMAL HUMAN SERA ; WEGENERS-GRANULOMATOSIS ; MICROSCOPIC POLYANGIITIS ; T-CELLS ; ANTIMYELOPEROXIDASE AUTOANTIBODIES ; AUTOIMMUNE GLOMERULONEPHRITIS ; CRESCENTIC GLOMERULONEPHRITIS ; CYTOPLASMIC AUTOANTIBODIES ; GOODPASTURE AUTOANTIGEN ; CRYPTIC EPITOPES
英文摘要

Anti-neutrophil cytoplasmic antibodies (ANCAs) have a pathogenic role in ANCA-associated vasculitis. The origin of ANCAs and anti-glomerular basement membrane (GBM) antibodies, however, is unknown. In this study, we determined whether natural autoantibodies against myeloperoxidase (MPO), proteinase 3 (PR3), and GBM were present in each of 10 healthy Chinese and Swedish individuals, negative for all three antigens by routine ELISA. Antibodies were purified from isolated IgG by antigen-specific affinity columns. Natural anti-GBM autoantibodies gave a linear staining pattern along the GBM of human renal sections. On ethanol-fixed granulocytes, both natural anti-MPO and anti-PR3 autoantibodies gave cytoplasmic staining. The titers of natural anti-MPO/PR3 autoantibodies were significantly lower than those from patients with vasculitis. In competition ELISA, the binding of natural anti-MPO autoantibodies could be inhibited by MPO, but not by PR3 or noncollagenous domains from type IV collagen. The same specificity results were found for natural anti-PR3 and anti-GBM autoantibodies. Overall, individuals of the Chinese origin had more natural autoantibodies than did those of the Swedish origin, but no other differences were found. Hence, our study shows that healthy individuals have masked circulating, noncross-reactive, antigen-specific natural autoantibodies against MPO, PR3, and GBM in their serum and IgG fractions. Further studies are needed to determine their role if any in the etiology of ANCA-associated vasculitis and anti-GBM disease. Kidney International (2010) 78, 590-597; doi:10.1038/ki.2010.198; published online 30 June 2010

语种英语
所属项目编号30725034 ; 30700752 ; 71X-15152
资助者National Natural Science Fund of China ; Swedish Research Council ; Lund University Hospital ; Crafoord Foundation ; National Natural Science Fund of China ; Swedish Research Council ; Lund University Hospital ; Crafoord Foundation
WOS记录号WOS:000281467100011
引用统计
被引频次:54[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51308
专题北京大学第一临床医学院_肾脏内科
作者单位1.Lund Univ, Dept Nephrol, SE-22184 Lund, Sweden
2.Peking Univ, Inst Nephrol, Hosp 1, Renal Div,Dept Med, Beijing 100871, Peoples R China
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Cui, Zhao,Zhao, Ming-hui,Segelmark, Marten,et al. Natural autoantibodies to myeloperoxidase, proteinase 3, and the glomerular basement membrane are present in normal individuals[J]. KIDNEY INTERNATIONAL,2010,78(6):590-597.
APA Cui, Zhao,Zhao, Ming-hui,Segelmark, Marten,&Hellmark, Thomas.(2010).Natural autoantibodies to myeloperoxidase, proteinase 3, and the glomerular basement membrane are present in normal individuals.KIDNEY INTERNATIONAL,78(6),590-597.
MLA Cui, Zhao,et al."Natural autoantibodies to myeloperoxidase, proteinase 3, and the glomerular basement membrane are present in normal individuals".KIDNEY INTERNATIONAL 78.6(2010):590-597.
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