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学科主题临床医学
Simultaneous quantification of tiloronoxim and tilorone in human urine by liquid chromatography-tandem mass spectrometry
Zhang, Xianhua1,2; Yang, Li1; Zhai, Suodi1; Duan, Jingli1,2
关键词Tiloronoxim Tilorone Lc-ms/ms Urine
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2008-11-15
DOI10.1016/j.jchromb.2008.09.011
875期:2页:349-357
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]HIGH-RESOLUTION CT ; SILICOSIS
英文摘要

A simple. sensitive and specific HPLC method with tandem mass spectrometry (HPLC/MS/MS) detection has been developed and validated for the simultaneous quantification of tiloronoxim and its major active metabolite, tilorone, in human urine. The analytes, together with metoprolol, which was employed as an internal standard (IS), were extracted with a mixture solvent of chloroform/ethyl ether (1/2, v/v). The chromatographic separation was performed on a narrow-bore reversed phase HPLC column with a gradient mobile phase of methanol/water containing 15 mM ammonium bicarbonate (pH 10.5). The API 3000 mass spectrometer was equipped with a TurbolonSpray interface and was operated on positive-ion, multiple reaction-monitoring (MRM) mode. The mass transitions monitored were m/z 426.3 -> 100.0, m/z 411.3 -> 100.0 and m/z 268.3 -> 116.1 for tiloronoxim, tilorone and the IS, respectively. The assay exhibited a linear dynamic range of 1-100ng/ml for both tiloronoxim and tilorone based on the analysis of 0.2 ml aliquots of urine. The lower limit of quantification was 1 ng/ml for both compounds. Acceptable precision and accuracies were obtained for concentrations over the standard Curve ranges. Run time of 8 min for each injection made it possible to analyze a high throughput Of Urine samples. The assay has been successfully used to analyze human urine samples from healthy volunteers. (C) 2008 Elsevier BY. All rights reserved.

语种英语
WOS记录号WOS:000261272900003
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51309
专题北京大学第三临床医学院_药剂科
作者单位1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100083, Peoples R China
2.Ctr Therapeut Drugs Monitoring & Clin Toxicol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Xianhua,Yang, Li,Zhai, Suodi,et al. Simultaneous quantification of tiloronoxim and tilorone in human urine by liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,875(2):349-357.
APA Zhang, Xianhua,Yang, Li,Zhai, Suodi,&Duan, Jingli.(2008).Simultaneous quantification of tiloronoxim and tilorone in human urine by liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,875(2),349-357.
MLA Zhang, Xianhua,et al."Simultaneous quantification of tiloronoxim and tilorone in human urine by liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 875.2(2008):349-357.
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