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Dynamic transcriptional symmetry-breaking in pre-implantation mammalian embryo development revealed by single-cell RNA-seq
Shi, Junchao1,2; Chen, Qi1,3; Li, Xin1; Zheng, Xiudeng1; Zhang, Ying1; Qiao, Jie4,5; Tang, Fuchou4,5; Tao, Yi1; Zhou, Qi1; Duan, Enkui1
关键词Transcriptional Symmetry-breaking Pre-implantation Embryo Development Lineage Divergence Monostable Model Bistable Model
刊名DEVELOPMENT
2015-10-15
DOI10.1242/dev.123950
142期:20页:3468-3477
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Developmental Biology
研究领域[WOS]Developmental Biology
关键词[WOS]EARLY MOUSE EMBRYO ; GENE REGULATORY NETWORKS ; TO-ZYGOTIC TRANSITION ; LINEAGE SPECIFIERS ; FATE DECISIONS ; MESSENGER-RNA ; STEM-CELLS ; EXPRESSION ; PLURIPOTENCY ; MASS
英文摘要

During mammalian pre-implantation embryo development, when the first asymmetry emerges and how it develops to direct distinct cell fates remain longstanding questions. Here, by analyzing single-blastomere transcriptome data from mouse and human pre-implantation embryos, we revealed that the initial blastomere-toblastomere biases emerge as early as the first embryonic cleavage division, following a binomial distribution pattern. The subsequent zygotic transcriptional activation further elevated overall blastomere-to-blastomere biases during the two-to 16-cell embryo stages. The trends of transcriptional asymmetry fell into two distinct patterns: for some genes, the extent of asymmetry was minimized between blastomeres (monostable pattern), whereas other genes, including those known to be lineage specifiers, showed ever-increasing asymmetry between blastomeres (bistable pattern), supposedly controlled by negative or positive feedbacks. Moreover, our analysis supports a scenario in which opposing lineage specifiers within an early blastomere constantly compete with each other based on their relative ratio, forming an inclined ′lineage strength′ that pushes the blastomere onto a predisposed, yet flexible, lineage track before morphological distinction.

语种英语
WOS记录号WOS:000366358700003
项目编号2015CB943000 ; 2011CB944401 ; XDA01000000 ; XDA04020202-20 ; 81490741 ; 31200879 ; 31300957 ; 11401562 ; 201306
资助机构National Basic Research Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Fellowship of Youth Innovation Promotion Association, Chinese Academy of Sciences
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51332
专题北京大学第三临床医学院
北京大学第三临床医学院_生殖医学中心
作者单位1.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Key Lab Anim Ecol & Conservat Biol, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Nevada, Sch Med, Dept Physiol & Cell Biol, Reno, NV 89557 USA
4.Peking Univ, Hosp 3, Coll Life Sci, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China
5.Peking Univ, Hosp 3, Coll Life Sci, Ctr Reprod Med, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Shi, Junchao,Chen, Qi,Li, Xin,et al. Dynamic transcriptional symmetry-breaking in pre-implantation mammalian embryo development revealed by single-cell RNA-seq[J]. DEVELOPMENT,2015,142(20):3468-3477.
APA Shi, Junchao.,Chen, Qi.,Li, Xin.,Zheng, Xiudeng.,Zhang, Ying.,...&Duan, Enkui.(2015).Dynamic transcriptional symmetry-breaking in pre-implantation mammalian embryo development revealed by single-cell RNA-seq.DEVELOPMENT,142(20),3468-3477.
MLA Shi, Junchao,et al."Dynamic transcriptional symmetry-breaking in pre-implantation mammalian embryo development revealed by single-cell RNA-seq".DEVELOPMENT 142.20(2015):3468-3477.
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