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学科主题: 临床医学
题名:
Dynamic transcriptional symmetry-breaking in pre-implantation mammalian embryo development revealed by single-cell RNA-seq
作者: Shi, Junchao1,2; Chen, Qi1,3; Li, Xin1; Zheng, Xiudeng1; Zhang, Ying1; Qiao, Jie4,5; Tang, Fuchou4,5; Tao, Yi1; Zhou, Qi1; Duan, Enkui1
关键词: Transcriptional symmetry-breaking ; Pre-implantation embryo development ; Lineage divergence ; Monostable model ; Bistable model
刊名: DEVELOPMENT
发表日期: 2015-10-15
DOI: 10.1242/dev.123950
卷: 142, 期:20, 页:3468-3477
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Developmental Biology
研究领域[WOS]: Developmental Biology
关键词[WOS]: EARLY MOUSE EMBRYO ; GENE REGULATORY NETWORKS ; TO-ZYGOTIC TRANSITION ; LINEAGE SPECIFIERS ; FATE DECISIONS ; MESSENGER-RNA ; STEM-CELLS ; EXPRESSION ; PLURIPOTENCY ; MASS
英文摘要:

During mammalian pre-implantation embryo development, when the first asymmetry emerges and how it develops to direct distinct cell fates remain longstanding questions. Here, by analyzing single-blastomere transcriptome data from mouse and human pre-implantation embryos, we revealed that the initial blastomere-toblastomere biases emerge as early as the first embryonic cleavage division, following a binomial distribution pattern. The subsequent zygotic transcriptional activation further elevated overall blastomere-to-blastomere biases during the two-to 16-cell embryo stages. The trends of transcriptional asymmetry fell into two distinct patterns: for some genes, the extent of asymmetry was minimized between blastomeres (monostable pattern), whereas other genes, including those known to be lineage specifiers, showed ever-increasing asymmetry between blastomeres (bistable pattern), supposedly controlled by negative or positive feedbacks. Moreover, our analysis supports a scenario in which opposing lineage specifiers within an early blastomere constantly compete with each other based on their relative ratio, forming an inclined ′lineage strength′ that pushes the blastomere onto a predisposed, yet flexible, lineage track before morphological distinction.

语种: 英语
所属项目编号: 2015CB943000 ; 2011CB944401 ; XDA01000000 ; XDA04020202-20 ; 81490741 ; 31200879 ; 31300957 ; 11401562 ; 201306
项目资助者: National Basic Research Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Fellowship of Youth Innovation Promotion Association, Chinese Academy of Sciences
WOS记录号: WOS:000366358700003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51332
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Key Lab Anim Ecol & Conservat Biol, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Nevada, Sch Med, Dept Physiol & Cell Biol, Reno, NV 89557 USA
4.Peking Univ, Hosp 3, Coll Life Sci, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China
5.Peking Univ, Hosp 3, Coll Life Sci, Ctr Reprod Med, Beijing 100871, Peoples R China

Recommended Citation:
Shi, Junchao,Chen, Qi,Li, Xin,et al. Dynamic transcriptional symmetry-breaking in pre-implantation mammalian embryo development revealed by single-cell RNA-seq[J]. DEVELOPMENT,2015,142(20):3468-3477.
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