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学科主题: 临床医学
题名:
Cloning and functional research of renal cell carcinoma related novel gene - GYLZ-RCC18
作者: Zhang, Q1; Chen, L1; Al, JK1; Lee, C1; Liang, LL1; Tong, M1; Zhang, ZW1; Na, YQ1; Guo, YL1
关键词: kidney neoplasm ; carcinoma ; gene ; GYLZ-RCC18
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2001-10-01
卷: 46, 期:19, 页:1622-1626
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要:

After the renal cell carcinoma related novel gene fragment GYLZ-RCC18 was cloned by using suppression subtractive hybridization (SSH), we used the SMART RACE technology to clone the full length of GYLZ-RCC18 and performed chromosome location by the FISH method. RT-PCR was used to detect the expression of the first reading frame of GYLZ-RCC18 in different stages and grades of renal cell carcinoma tissue and other tissues. Also we transfected the antisense oligonucleotide of GYLZ-RCC18 to renal cell carcinoma cell line GRC-1, and analyzed the proliferation activity, growth speed, apoptosis and mortality changes in GRC-1. The results show that the full length of GYLZ-RCC18 (GenBank accession No.: BE825133) cDNA is about 3.5 kb long which is located at No. 14 chromosome. GYLZ-RCC18 has a higher expression in higher grades and stages of renal cell carcinoma than in the lower ones. The expression of GYLZ-RCC18 in renal cell carcinoma was much higher than that in normal kidney and other tissues. After transfection of GYLZ-RCC18 antisense oligonucleotide, the mortality of GRC-1 increases evidently, the proliferation activity and growth speed were inhibited remarkably at the same time. Also the antisense oligonucleotide can induce the apoptosis of GRC-1 all through the observation time. Our results indicated that GYLZ-RCC18 is an important novel gene related to renal cell carcinoma. Its overexpression would stimulate the growth and proliferation activity and plays an antidead and antiapoptosis effect in renal cell carcinoma. Transfection of antisense oligonucleotide could inhibit the generation and development of renal cell carcinoma. The study provides a new clue for the research of renal cell carcinoma, and also provides an instruction for special genetic diagnosis and the therapy of renal cell carcinoma.

语种: 英语
WOS记录号: WOS:000172324000012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51339
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Dept Physiol, Beijing 100083, Peoples R China
2.Northwestern Univ, Sch Med, Evanston, IL 60208 USA
3.Peking Univ, Dept Urol, Beijing 100034, Peoples R China
4.Union Hosp, Fujian Haematol Inst, Fuzhou 350001, Peoples R China
5.Peking Univ, Affiliated Hosp 1, Inst Urol, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, Q,Chen, L,Al, JK,et al. Cloning and functional research of renal cell carcinoma related novel gene - GYLZ-RCC18[J]. CHINESE SCIENCE BULLETIN,2001,46(19):1622-1626.
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