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P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells
Zhou, Lingli; Cai, Xiaoling; Han, Xueyao; Ji, Linong
刊名JOURNAL OF DIABETES RESEARCH
2014
DOI10.1155/2014/834528
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism ; Medicine, Research & Experimental
研究领域[WOS]Endocrinology & Metabolism ; Research & Experimental Medicine
关键词[WOS]TYPE-2 ; DEATH
英文摘要

Objectives. The mechanism underlying the regulation of glucolipotoxicity-induced apoptosis by MAPKs was examined in INS-1 cells. Methods. The rat insulinoma cell line INS-1 was cotreated with glucose (30mM) and palmitic acid (0.2mM) (GLU+PA). Apoptosis was assessed by cell morphology and detection of PARP cleavage. The activation of MAPKs was examined by Western blotting using specific antibodies against the phosphorylated forms of JNK, ERK1/2, and P38. Results. (1) Live cell imaging studies showed that treatment with GLU+PA for 72 h induced significant cell death, concomitant with PARP-1 cleavage and caspase-3 activation, which peaked at 96 h of treatment. (2) Western blot analysis of the activation of MAPKs during GLU+PA-induced INS-1 cell apoptosis showed that phosphorylation of P38 increased gradually and reached a peak at 96 h, which coincided with PARP-1 cleavage. A transient increase of ERK activation was followed by a rapid decline at 96 h, whereas JNK phosphorylation status remained unchanged in response to GLU+PA. (3) Phosphorylation of insulin receptor substrate (IRS)-2 at 48 h of treatment triggered its degradation, which coincided with P38 activation. (4) Inhibition of P38, but not JNK or ERK, blocked GLU+PA-induced INS-1 cell apoptosis. Conclusions. P38 may be involved in the regulation of glucolipotoxicity-induced apoptosis through the phosphorylation of IRS-2.

语种英语
WOS记录号WOS:000332857900001
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51348
专题北京大学第二临床医学院
北京大学第二临床医学院_内分泌科
作者单位Peking Univ, Peoples Hosp, Dept Endocrinol & Metab, Beijing 100044, Peoples R China
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Zhou, Lingli,Cai, Xiaoling,Han, Xueyao,et al. P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells[J]. JOURNAL OF DIABETES RESEARCH,2014.
APA Zhou, Lingli,Cai, Xiaoling,Han, Xueyao,&Ji, Linong.(2014).P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells.JOURNAL OF DIABETES RESEARCH.
MLA Zhou, Lingli,et al."P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells".JOURNAL OF DIABETES RESEARCH (2014).
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