北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Identification and Determination of the Major Constituents in the Traditional Uighur Medicinal Plant Saussurea involucrata by LC-DAD-MS
作者: Yi, Tao1; Chen, Hu-Biao1; Zhao, Zhong-Zhen1; Jiang, Zhi-Hong1; Cai, Shao-Qing2; Wang, Tian-Min2
关键词: Column liquid chromatography ; Diode array detection ; Electrospray ionisation mass spectrometry ; Pharmaceutical analysis ; Ethnopharmacy ; Saussurea involucrata
刊名: CHROMATOGRAPHIA
发表日期: 2009-03-01
DOI: 10.1365/s10337-008-0923-9
卷: 69, 期:5-6, 页:537-542
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: LIGUSTICUM-CHUANXIONG ; HPLC ; GUAIANOLIDES ; GLUCOSIDES ; ESI/MS
英文摘要:

A liquid chromatography coupled with diode array detector and electrospray ionization mass spectrometry method was developed for the simultaneous analysis of the major constituents in Saussurea involucrata (SI). A comprehensive validation of the developed method was conducted, and the unique properties of the present method were confirmed by analyzing 11 SI samples. Seventeen compounds including phenolic acids, flavonoids, and lignanoids were identified by online ESI-MS and by comparison with known data in the literature and standard compounds; of these, eight were simultaneously quantified by LC-DAD. All linear regressions were acquired with R (2) > 0.99, and the limits of detection ranged from 0.85 to 3.03 ng. Repeatability was evaluated by intra- and inter-day assays; the relative standard deviation (RSD) value was within 4.95%. Recovery studies for the quantified compounds were found to be within the range 97.31-101.17% with an RSD of less than 2.18%. Overall, the present hyphenation procedure is highly efficient and reliable, and hence suitable for qualitative and quantitative analysis of a large number of samples.

语种: 英语
所属项目编号: FRG/07-08/II-38
项目资助者: Faculty Research Grant of Hong Kong Baptist University
WOS记录号: WOS:000263826600020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51349
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Yi, Tao,Chen, Hu-Biao,Zhao, Zhong-Zhen,et al. Identification and Determination of the Major Constituents in the Traditional Uighur Medicinal Plant Saussurea involucrata by LC-DAD-MS[J]. CHROMATOGRAPHIA,2009,69(5-6):537-542.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yi, Tao]'s Articles
[Chen, Hu-Biao]'s Articles
[Zhao, Zhong-Zhen]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yi, Tao]‘s Articles
[Chen, Hu-Biao]‘s Articles
[Zhao, Zhong-Zhen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace