北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Angiogenesis inhibition and cell cycle arrest induced by treatment with Pseudolarix acid B alone or combined with 5-fluorouracil
作者: Liu, Jingtao; Guo, Wei; Xu, Bo; Ran, Fuxiang; Chu, Mingming; Fu, Hongzheng; Cui, Jingrong
关键词: combination therapy ; angiogenesis inhibition ; cell cycle arrest ; Pseudolarix acid B ; 5-fluorouracil
刊名: ACTA BIOCHIMICA ET BIOPHYSICA SINICA
发表日期: 2012-06-01
DOI: 10.1093/abbs/gms029
卷: 44, 期:6, 页:490-502
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; TUMOR ANGIOGENESIS ; APOPTOSIS ; CANCER ; OVEREXPRESSION ; CHEMOTHERAPY ; HYPOXIA ; AGENT ; MODEL ; VEGF
英文摘要:

Angiogenesis inhibitors combined with chemotherapeutic drugs have significant efficacy in the treatment of a variety of cancers. Pseudolarix acid B (PAB) is a traditional pregnancy-terminating agent, which has previously been shown to reduce tumor growth and angiogenesis. In this study, we used the high content screening assay to examine the effects of PAB on human umbilical vein endothelial cells (HUVECs). Two hepatocarcinoma 22-transplanted mouse models were used to determine PAB efficacy in combination with 5-fluorouracil (5-Fu). Our results suggested that PAB (0.1561.250 M) inhibited HUVECs motility in a concentration-dependent manner without obvious cytotoxicity in vitro. In vivo, PAB (25 mg/kg/day) promoted the anti-tumor efficacy of 5-Fu (5 mg/kg/2 days) in combination therapy, resulting in significantly higher tumor inhibition rates, lower microvessel density values, and prolonged survival times. It was also demonstrated that PAB acted by blocking the cell cycle at both the G(1)/S boundary and M phase, down-regulation of vascular endothelial growth factor, hypoxia-inducible factor 1 and cyclin E expression, and up-regulation of cdc2 expression. These observations provide the first evidence that PAB in combination with 5-Fu may be useful in cancer treatment.

语种: 英语
所属项目编号: 2009ZX09301-010
项目资助者: Comprehensive Center for Drug Discovery and Development, Peking University
WOS记录号: WOS:000304830700004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51350
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Liu, Jingtao,Guo, Wei,Xu, Bo,et al. Angiogenesis inhibition and cell cycle arrest induced by treatment with Pseudolarix acid B alone or combined with 5-fluorouracil[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(6):490-502.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Jingtao]'s Articles
[Guo, Wei]'s Articles
[Xu, Bo]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Jingtao]‘s Articles
[Guo, Wei]‘s Articles
[Xu, Bo]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace