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学科主题临床医学
Dynamic expression of microRNAs during the differentiation of human embryonic stem cells into insulin-producing cells
Wei, Rui1,3; Yang, Jin1,3; Liu, Guo-qiang1,3; Gao, Mei-juan1,3; Hou, Wen-fang1,3; Zhang, Lin1,3; Gao, Hong-wei1,3; Liu, Ye1,3; Chen, Gui-an2,3; Hong, Tian-pei1,3
关键词Microrna Embryonic Stem Cells Differentiation Pancreatic Islet Cells
刊名GENE
2013-04-15
DOI10.1016/j.gene.2013.01.038
518期:2页:246-255
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Genetics & Heredity
研究领域[WOS]Genetics & Heredity
关键词[WOS]PANCREATIC BETA-CELLS ; TIME QUANTITATIVE PCR ; PROFILING REVEALS ; MOUSE ; ISLETS ; TARGETS ; GROWTH ; TISSUE ; LINES ; FATE
英文摘要

Human embryonic stem (hES) cells with the capacity of self-renewal and multilineage differentiation are promising sources for generation of pancreatic islet cells for cell replacement therapy in diabetes. Here we induced hES cells into insulin-producing cells (IPCs) in a stepwise process which recapitulated islet organogenesis by directing cells through the stages resembling definitive endoderm, gut-tube endoderm, pancreatic precursor and cells that expressed pancreatic endocrine hormones. The dynamic expression of microRNAs (miRNAs) during the differentiation was analyzed and was compared with that in the development of human pancreatic islets. We found that the dynamic expression patterns of miR-375 and miR-7 were similar to those seen in the development of human fetal pancreas, whereas the dynamic expression of miR-146a and miR-34a showed specific patterns during the differentiation. Furthermore, the expression of Hnf1 beta and Pax6, the predicted target genes of miR-375 and miR-7, was reciprocal to that of miR-375 and miR-7. Overexpression of miR-375 down-regulated the expression of gut-endoderm/pancreatic progenitor specific markers Hnf1 beta and Sox9. Therefore, the miRNAs may directly or indirectly regulate the expression of pancreatic islet organogenesis-specific transcription factors to control the differentiation and maturation of pancreatic islet cells. (c) 2013 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000317800700004
项目编号81070701 ; 81000315 ; 81270858 ; 2006AA02A112 ; 7123234 ; 2012CB517502 ; 20100001110083 ; 20120001120063 ; 20120001120069 ; 20120001120076
资助机构National Natural Sciences Foundation of China ; Chinese National 863 Program ; Natural Science Foundation of Beijing City ; Chinese National 973 Program ; Specialized Research Fund for the Doctoral Program of Higher Education
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51363
专题北京大学第三临床医学院
北京大学第三临床医学院_内分泌科
作者单位1.Peking Univ, Dept Endocrinol & Metab, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Reprod Med Ctr, Hosp 3, Beijing 100191, Peoples R China
3.Peking Univ, Clin Stem Cell Res Ctr, Hosp 3, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Wei, Rui,Yang, Jin,Liu, Guo-qiang,et al. Dynamic expression of microRNAs during the differentiation of human embryonic stem cells into insulin-producing cells[J]. GENE,2013,518(2):246-255.
APA Wei, Rui.,Yang, Jin.,Liu, Guo-qiang.,Gao, Mei-juan.,Hou, Wen-fang.,...&Hong, Tian-pei.(2013).Dynamic expression of microRNAs during the differentiation of human embryonic stem cells into insulin-producing cells.GENE,518(2),246-255.
MLA Wei, Rui,et al."Dynamic expression of microRNAs during the differentiation of human embryonic stem cells into insulin-producing cells".GENE 518.2(2013):246-255.
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