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学科主题临床医学
CTRP3 Stimulates Proliferation and Anti-Apoptosis of Prostate Cells through PKC Signaling Pathways
Hou, Qi1; Lin, Jinyan2; Huang, Wentao3; Li, Maoyin3; Feng, Jianhua1; Mao, Xiangming4
刊名PLOS ONE
2015-07-28
DOI10.1371/journal.pone.0134006
10期:7
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]C1Q/TNF-RELATED PROTEIN-3 CTRP3 ; PROMOTES PROLIFERATION ; ADIPOSE-TISSUE ; CTRP3/CARTDUCIN ; PARALOG ; METABOLISM ; EXPRESSION ; CARTDUCIN ; ADIPOKINE ; CORS-26
英文摘要

C1q/TNF-related protein-3 (CTRP3) is a novel adipokine with roles in multiple cellular processes. However, little is known about its function in prostate cells. This study investigated the effects and mechanisms of CTRP3 in prostate cells. We first generated and purified CTRP3 protein in HEK 293T cells. Proliferation of RWPE-1 prostate cells was evaluated by MTT analyses under treatment with different concentrations of CTRP3 for various exposure times. The results revealed maximum enhancement of proliferation with 10 mu g/mL CTRP3 for 72 h. Cell apoptosis and cell cycle were determined by TUNEL staining and flow cytometry analysis. TUNEL assay showed decreased TUNEL-positive cells in RWPE-1 prostate cells treated with CTRP3, and flow cytometry showed significantly decreased apoptotic cells upon CTRP3 treatment (treated cells, 8.34 +/- 1.175 vs. controls, 20.163 +/- 0.35) (P < 0.01). Moreover, flow cytometry analysis also showed a significant decrease of cells in the G1 phase and an increase of cells in the S and G2 phase upon CTRP3 treatment (treated cells, 42.85 +/- 1.40 vs. control, 52.77 +/- 0.90; 28.41 +/- 0.57 vs. 23.49 +/- 1.13; 27.08 +/- 1.97 vs. 22.20 +/- 1.32, respectively) (all P < 0.05). Two-dimensional gel electrophoresis and mass spectrometry identified differentially expressed proteins, including cytokeratin-19, GLRX3 and DDAH1, which were upregulated in CTRP3 treated cells, and cytokeratin-17 and 14-3-3 sigma, which were downregulated. GLRX3, DDAH1 and 14-3-3 sigma were confirmed using western blot analysis. A PKC inhibitor, staurosporine, was used to inhibit PKC activity in CTRP3 treated RWPE-1 cells. Staurosporine completely abolished the CTRP3-induced increased phosphorylation of intracellular PKC substrates and CTRP3-stimulated effect by RWPE-1 cells. Our results provide the first evidence for a physiological role of the novel adipokine, CTRP3, in prostate cells. Our findings suggest that CTRP3 could improve proliferation and anti-apoptosis of prostate cells through protein kinase C signaling pathways.

语种英语
WOS记录号WOS:000358595900063
项目编号201406093001004
资助机构Medical Science and Technology Program of Shenzhen Longgang district
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51365
专题北京大学深圳医院_泌尿外科
作者单位1.Longgang Dist Cent Hosp, Dept Urol, Shenzhen, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen, Peoples R China
3.Southern Med Univ, Nanfang Hosp, Hlth management Ctr, Guangzhou, Guangdong, Peoples R China
4.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Urol, Guangzhou 510275, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Hou, Qi,Lin, Jinyan,Huang, Wentao,et al. CTRP3 Stimulates Proliferation and Anti-Apoptosis of Prostate Cells through PKC Signaling Pathways[J]. PLOS ONE,2015,10(7).
APA Hou, Qi,Lin, Jinyan,Huang, Wentao,Li, Maoyin,Feng, Jianhua,&Mao, Xiangming.(2015).CTRP3 Stimulates Proliferation and Anti-Apoptosis of Prostate Cells through PKC Signaling Pathways.PLOS ONE,10(7).
MLA Hou, Qi,et al."CTRP3 Stimulates Proliferation and Anti-Apoptosis of Prostate Cells through PKC Signaling Pathways".PLOS ONE 10.7(2015).
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