IR@PKUHSC  > 基础医学院  > 生理学与病理生理学系
学科主题基础医学
C-reactive protein augments interleukin-8 secretion in human peripheral blood monocytes
Xie, LQ1; Chang, L1; Guan, YF1; Wang, X1
关键词atherosclerosis G-reactive protein inflammation macrophages
刊名JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
2005-11-01
46期:5页:690-696
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems ; Pharmacology & Pharmacy
研究领域[WOS]Cardiovascular System & Cardiology ; Pharmacology & Pharmacy
关键词[WOS]VASCULAR SMOOTH-MUSCLE ; HUMAN ENDOTHELIAL-CELLS ; FACTOR-KAPPA-B ; CHEMOATTRACTANT PROTEIN-1 ; OXIDATIVE STRESS ; INFLAMMATORY RESPONSE ; ALVEOLAR MACROPHAGES ; ATHEROMATOUS PLAQUES ; SYNTHETIC PEPTIDE ; UNSTABLE ANGINA
英文摘要

C-reactive protein (CRP) is a powerful predictor and risk factor for cardiovascular diseases. The CXC- and CC-type chemokines interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) are important chemokines for leukocyte trafficking identified in atheromatous plaque expressed mainly by macrophages in humans. We assessed whether C-reactive protein could induce MCP-1 and IL-8 secretion. In human peripheral blood monocytes, C-reactive protein (12.5-50 mu g/mL) increased IL-8, but not MCP-1 secretion in a time- (6-24 hours) and dose-dependent manner as detected by ELISA. C-reactive protein could augment the production of reactive oxygen species (ROS) as measured by chemiluminescence and inhibitors of NAD(P)H oxidase (DPI and PAO) and ROS scavengers (superoxide dismutase, catalase, and 1% dimethyl sulphoxide) abolished C-reactive protein-induced IL-8 secretion. Furthermore, relative quantity of IL-8 mRNA was significantly increased by C-reactive protein 50 mu g/mL for 12 hours, which could be inhibited by DPI 1 mu M or superoxide dismutase (SOD) 250 U/mL. The inhibitors of ERK 1/2 (PD98059), p38 (SB203580) MAPK, and NF-kappa B (PDTC and MG132) significantly decreased C-reactive protein-induced IL-8 secretion in human monocytes. Also, agonists of peroxisome proliferator-activated receptor (PPAR) alpha (WY14643) and PPAR-gamma (troglitazone) could largely inhibit C-reactive protein responses. Thus, our data indicate that C-reactive protein at pathologic levels increases IL-8 secretion and mRNA via enhancing ROS derived mainly from NAD(P)H oxidase and the subsequent activation of ERK1/2, p38 MAPK, and NF-kappa B. The activation of PPAR alpha/gamma can negatively regulate C-reactive protein-induced IL-8 production in human monocytes.

语种英语
WOS记录号WOS:000235808400017
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51380
专题基础医学院_生理学与病理生理学系
作者单位1.Peking Univ, Basic Med Coll, Dept Physiol, Beijing 100083, Peoples R China
2.Peking Univ, Basic Med Coll, Key Lab Mol Cardiovasc Sci, Educ Minist, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Xie, LQ,Chang, L,Guan, YF,et al. C-reactive protein augments interleukin-8 secretion in human peripheral blood monocytes[J]. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY,2005,46(5):690-696.
APA Xie, LQ,Chang, L,Guan, YF,&Wang, X.(2005).C-reactive protein augments interleukin-8 secretion in human peripheral blood monocytes.JOURNAL OF CARDIOVASCULAR PHARMACOLOGY,46(5),690-696.
MLA Xie, LQ,et al."C-reactive protein augments interleukin-8 secretion in human peripheral blood monocytes".JOURNAL OF CARDIOVASCULAR PHARMACOLOGY 46.5(2005):690-696.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
C-reactive protein a(246KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xie, LQ]的文章
[Chang, L]的文章
[Guan, YF]的文章
百度学术
百度学术中相似的文章
[Xie, LQ]的文章
[Chang, L]的文章
[Guan, YF]的文章
必应学术
必应学术中相似的文章
[Xie, LQ]的文章
[Chang, L]的文章
[Guan, YF]的文章
相关权益政策
暂无数据
收藏/分享
文件名: C-reactive protein augments interleukin-8 secretion in human peripheral blood monocytes.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。