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学科主题: 临床医学
题名:
Effects of substance P on growth of fibroblast-like cells derived from bile duct: an in vitro cell culture study
作者: Tian Yuanhu1,2; Yang Guangyun1,2; Zhang Xiaoqing2; Shen Wei2; Dong Jiahong1; Xu Zhi2
关键词: substance P ; bile duct ; fibroblast ; cell culture ; cell proliferation
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2014-09-05
DOI: 10.3760/cma.j.issn.0366-6999.20140765
卷: 127, 期:17, 页:3121-3126
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: BILIARY STRICTURE ; NK-1 RECEPTOR ; EXPRESSION ; MODEL ; NEUROPEPTIDES ; CHONDROCYTES
英文摘要:

Background The possible role of substance P (SP) during wound healing has been the primary research focus in recent years, but its effect on the healing process after bile duct injury is little understood. This study aimed to investigate the effects of SP on growth of fibroblast-like cells derived from rabbit bile duct.

Methods Fibroblast-like cells derived from rabbit bile duct were identified and divided randomly into control and experimental groups. SP-treated cells at different concentrations of 10(-9)-10(-5) mol/L and control group were incubated, respectively, for 48 hours. After incubating, the effects of SP on cell proliferation were assessed by cell counts and MTT test. Apoptosis rate (AR) of cells was measured by flow cytometry.

Results Cultured rabbit bile duct cells were fibroblast-like in morphology, and these cells were-stained positively for vimentin and negatively for desmin. After SP was added to nonconfluent cells for 48 hours, cell numbers were significantly increased in experimental groups than in controls (P <0.05). The maximum stimulation of cell proliferation was achieved at SP of 10(-5) mol/L. Bile duct fibroblast-like cells in the SP group showed a higher proliferating activity and lower AR than those in the control group or in the SP + Spantide group (P <0.05). Spantide partly inhibited the effects of SP on fibroblast-like cells. Examination under transmission electron microscopy revealed rough endoplasmic reticulum and prominent Golgi complexes after SP treatment.

Conclusions SP has a growth regulatory property on cultivated bile duct fibroblast-like cells in vitro, suggesting that SP may involve in wound healing after bile duct injury by promoting wound fibroblast proliferation and inhibiting apoptosis and participate in pathological scar formation.

语种: 英语
所属项目编号: 2007AA04Z313 ; 201003781
项目资助者: National High Technology Research and Development Program of China (863 Program) ; China Postdoctoral Science Foundation
WOS记录号: WOS:000343337100017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51411
Appears in Collections:北京大学第三临床医学院_普通外科_期刊论文

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作者单位: 1.Chinese Peoples Liberat Army Gen Hosp, Dept Hepatobiliary Surg, Beijing 100853, Peoples R China
2.Peking Univ, Hosp 3, Dept Gen Surg, Beijing 100191, Peoples R China

Recommended Citation:
Tian Yuanhu,Yang Guangyun,Zhang Xiaoqing,et al. Effects of substance P on growth of fibroblast-like cells derived from bile duct: an in vitro cell culture study[J]. CHINESE MEDICAL JOURNAL,2014,127(17):3121-3126.
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