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学科主题: 临床医学
题名:
The reversal of hyperglycaemia in diabetic mice using PLGA scaffolds seeded with islet-like cells derived from human embryonic stem cells
作者: Mao, Gen-hong1,2; Chen, Gui-an1,2; Bai, Hai-yan1,2; Song, Tian-ran1,2; Wang, Yan-xia1,2
关键词: Human embryonic stem cells ; Islet ; Scaffold ; Transplantation
刊名: BIOMATERIALS
发表日期: 2009-03-01
DOI: 10.1016/j.biomaterials.2008.12.030
卷: 30, 期:9, 页:1706-1714
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: INSULIN-PRODUCING CELLS ; SECRETING CELLS ; PANCREATIC-ISLETS ; PEPTIDE-1 DIFFERENTIATION ; DIRECTED DIFFERENTIATION ; MOUSE PANCREAS ; EXPRESSION ; GENERATION ; PROGENITOR ; ENDOCRINE
英文摘要:

Islet-like cells derived from embryonic stem (ES) cells may be a promising therapeutic option for future diabetes treatment. Here, we demonstrated a five-stage protocol with adding exendin-4 instead of nicotinamide finally could generate islet-like cells from human embryonic stem (ES) cells. Immunofluorescence analysis revealed a high percentage of c-peptide positive cells in the derivation. However, in addition to insulin/c-peptide, most cells also coexpressed PDX-1 (pancreas duodenum homeobox-1), glucagon, somatostatin or pancreatic polypeptide. Insulin and other pancreatic beta-cell-specific genes were all present in the differentiated cells. Insulin secretion could be detected and increased significantly by adding KCL in high glucose concentration in vitro. Furthermore, subcutaneous transplantation of scaffolds seeded with the islet-like cells or cell transplantation under kidney capsules for further differentiation in vivo could improve 6 h fasted blood glucose levels and diabetic phenotypes in streptozotocin-induced diabetic SCID mice. More interestingly, blood vessels of host origin, characterized by mouse CD31 immunostaining, invaded the cell-scaffold complexes. This work reveals a five-stage protocol with adding exendin-4 may be an effective protocol on the differentiation of human ES cells into islet-like cells, and suggests scaffolds can serve as vehicles for islet-like cell transplantation. (C) 2008 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: PKU1.1
项目资助者: ES cell line
WOS记录号: WOS:000263742800006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51429
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Clin Stem Cell Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Mao, Gen-hong,Chen, Gui-an,Bai, Hai-yan,et al. The reversal of hyperglycaemia in diabetic mice using PLGA scaffolds seeded with islet-like cells derived from human embryonic stem cells[J]. BIOMATERIALS,2009,30(9):1706-1714.
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