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学科主题临床医学
The reversal of hyperglycaemia in diabetic mice using PLGA scaffolds seeded with islet-like cells derived from human embryonic stem cells
Mao, Gen-hong1,2; Chen, Gui-an1,2; Bai, Hai-yan1,2; Song, Tian-ran1,2; Wang, Yan-xia1,2
关键词Human Embryonic Stem Cells Islet Scaffold Transplantation
刊名BIOMATERIALS
2009-03-01
DOI10.1016/j.biomaterials.2008.12.030
30期:9页:1706-1714
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
资助者ES cell line ; ES cell line
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]INSULIN-PRODUCING CELLS ; SECRETING CELLS ; PANCREATIC-ISLETS ; PEPTIDE-1 DIFFERENTIATION ; DIRECTED DIFFERENTIATION ; MOUSE PANCREAS ; EXPRESSION ; GENERATION ; PROGENITOR ; ENDOCRINE
英文摘要

Islet-like cells derived from embryonic stem (ES) cells may be a promising therapeutic option for future diabetes treatment. Here, we demonstrated a five-stage protocol with adding exendin-4 instead of nicotinamide finally could generate islet-like cells from human embryonic stem (ES) cells. Immunofluorescence analysis revealed a high percentage of c-peptide positive cells in the derivation. However, in addition to insulin/c-peptide, most cells also coexpressed PDX-1 (pancreas duodenum homeobox-1), glucagon, somatostatin or pancreatic polypeptide. Insulin and other pancreatic beta-cell-specific genes were all present in the differentiated cells. Insulin secretion could be detected and increased significantly by adding KCL in high glucose concentration in vitro. Furthermore, subcutaneous transplantation of scaffolds seeded with the islet-like cells or cell transplantation under kidney capsules for further differentiation in vivo could improve 6 h fasted blood glucose levels and diabetic phenotypes in streptozotocin-induced diabetic SCID mice. More interestingly, blood vessels of host origin, characterized by mouse CD31 immunostaining, invaded the cell-scaffold complexes. This work reveals a five-stage protocol with adding exendin-4 may be an effective protocol on the differentiation of human ES cells into islet-like cells, and suggests scaffolds can serve as vehicles for islet-like cell transplantation. (C) 2008 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号PKU1.1
资助者ES cell line ; ES cell line
WOS记录号WOS:000263742800006
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51429
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Clin Stem Cell Ctr, Beijing 100191, Peoples R China
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GB/T 7714
Mao, Gen-hong,Chen, Gui-an,Bai, Hai-yan,et al. The reversal of hyperglycaemia in diabetic mice using PLGA scaffolds seeded with islet-like cells derived from human embryonic stem cells[J]. BIOMATERIALS,2009,30(9):1706-1714.
APA Mao, Gen-hong,Chen, Gui-an,Bai, Hai-yan,Song, Tian-ran,&Wang, Yan-xia.(2009).The reversal of hyperglycaemia in diabetic mice using PLGA scaffolds seeded with islet-like cells derived from human embryonic stem cells.BIOMATERIALS,30(9),1706-1714.
MLA Mao, Gen-hong,et al."The reversal of hyperglycaemia in diabetic mice using PLGA scaffolds seeded with islet-like cells derived from human embryonic stem cells".BIOMATERIALS 30.9(2009):1706-1714.
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