IR@PKUHSC  > 北京大学药学院  > 化学生物学系
学科主题药学
Endothelial cell and macrophage regulation of vascular smooth muscle cell calcification modulated by cholestane-3 beta, 5 alpha, 6 beta-triol
Liu, Hongmei1; Yuan, Lan1; Xu, Shanjin1; Wang, Kui1
关键词Vascular Calcification Endothelial Cells Macrophages Cholestane-3 Beta 5 Alpha 6 Beta-triol
刊名CELL BIOLOGY INTERNATIONAL
2007-09-01
DOI10.1016/j.cellbi.2007.02.009
31期:9页:900-907
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]MATRIX GLA PROTEIN ; HORMONE-RELATED PEPTIDE ; NECROSIS-FACTOR-ALPHA ; IN-VITRO ; DIFFERENTIATION ; ATHEROSCLEROSIS ; OSTEOPONTIN ; EXPRESSION ; SECRETION ; ARTERIES
英文摘要

The cellular and molecular mechanisms that mediate vascular calcification remain poorly understood. In our previous study, oxysterol cholestane-3 beta, 5 alpha, 6 beta-triol (Triol) was shown to promote vascular smooth muscle cells (VSMCs) calcification. In this study, by using direct coculture, non-contact transwell coculture, and culture with conditioned media, we investigated the roles of endothelial cells (ECs) and macrophages in the regulation of VSMCs calcification in the absence or presence of Triol. In vitro calcification was induced by incubation of VSMCs with beta-glycerophosphate. The results showed that ECs inhibited VSMCs calcification, as manifested by the reduction of calcium deposition in extracellular matrix, This effect of ECs on calcification was via the secreted soluble factors. Furthermore, the stimulation of ECs by Triol had no influence on ECs inhibition of calcification. On the other hand, macrophages promoted VSMCs calcification via the secreted soluble factors such as reactive oxygen species, which was further enhanced by Triol. Our results supported the roles for ECs and macrophages in vascular calcification, modulated by oxysterols in atherosclerotic plaque. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000247463300006
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51447
Collection北京大学药学院_化学生物学系
作者单位1.Huazhong Univ Sci & Technol, Dept Chem, Wuhan 430074, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
3.Peking Univ, Med & Healthy Anal Ctr, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Liu, Hongmei,Yuan, Lan,Xu, Shanjin,et al. Endothelial cell and macrophage regulation of vascular smooth muscle cell calcification modulated by cholestane-3 beta, 5 alpha, 6 beta-triol[J]. CELL BIOLOGY INTERNATIONAL,2007,31(9):900-907.
APA Liu, Hongmei,Yuan, Lan,Xu, Shanjin,&Wang, Kui.(2007).Endothelial cell and macrophage regulation of vascular smooth muscle cell calcification modulated by cholestane-3 beta, 5 alpha, 6 beta-triol.CELL BIOLOGY INTERNATIONAL,31(9),900-907.
MLA Liu, Hongmei,et al."Endothelial cell and macrophage regulation of vascular smooth muscle cell calcification modulated by cholestane-3 beta, 5 alpha, 6 beta-triol".CELL BIOLOGY INTERNATIONAL 31.9(2007):900-907.
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