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学科主题: 药学
题名:
Endothelial cell and macrophage regulation of vascular smooth muscle cell calcification modulated by cholestane-3 beta, 5 alpha, 6 beta-triol
作者: Liu, Hongmei1; Yuan, Lan1; Xu, Shanjin1; Wang, Kui1
关键词: vascular calcification ; endothelial cells ; macrophages ; cholestane-3 beta ; 5 alpha ; 6 beta-triol
刊名: CELL BIOLOGY INTERNATIONAL
发表日期: 2007-09-01
DOI: 10.1016/j.cellbi.2007.02.009
卷: 31, 期:9, 页:900-907
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: MATRIX GLA PROTEIN ; HORMONE-RELATED PEPTIDE ; NECROSIS-FACTOR-ALPHA ; IN-VITRO ; DIFFERENTIATION ; ATHEROSCLEROSIS ; OSTEOPONTIN ; EXPRESSION ; SECRETION ; ARTERIES
英文摘要:

The cellular and molecular mechanisms that mediate vascular calcification remain poorly understood. In our previous study, oxysterol cholestane-3 beta, 5 alpha, 6 beta-triol (Triol) was shown to promote vascular smooth muscle cells (VSMCs) calcification. In this study, by using direct coculture, non-contact transwell coculture, and culture with conditioned media, we investigated the roles of endothelial cells (ECs) and macrophages in the regulation of VSMCs calcification in the absence or presence of Triol. In vitro calcification was induced by incubation of VSMCs with beta-glycerophosphate. The results showed that ECs inhibited VSMCs calcification, as manifested by the reduction of calcium deposition in extracellular matrix, This effect of ECs on calcification was via the secreted soluble factors. Furthermore, the stimulation of ECs by Triol had no influence on ECs inhibition of calcification. On the other hand, macrophages promoted VSMCs calcification via the secreted soluble factors such as reactive oxygen species, which was further enhanced by Triol. Our results supported the roles for ECs and macrophages in vascular calcification, modulated by oxysterols in atherosclerotic plaque. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000247463300006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51447
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Huazhong Univ Sci & Technol, Dept Chem, Wuhan 430074, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
3.Peking Univ, Med & Healthy Anal Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Liu, Hongmei,Yuan, Lan,Xu, Shanjin,et al. Endothelial cell and macrophage regulation of vascular smooth muscle cell calcification modulated by cholestane-3 beta, 5 alpha, 6 beta-triol[J]. CELL BIOLOGY INTERNATIONAL,2007,31(9):900-907.
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