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学科主题药学
Production of secoisolariciresinol from defatted flaxseed by bacterial biotransformation
Li, M. -X.1; Zhu, H. -Y.2; Yang, D. -H.1; Ma, X. -Q.1; Wang, C. -Z.2; Cai, S. -Q.1; Liu, G. -R.3; Ku, B. -S.2; Liu, S. -L.2,3,4
关键词Bacteroides Uniformis Biotransformation Flaxseed Intestinal Bacteria Secoisolariciresinol Secoisolariciresinol Diglucoside
刊名JOURNAL OF APPLIED MICROBIOLOGY
2012-12-01
DOI10.1111/j.1365-2672.2012.05436.x
113期:6页:1352-1361
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Microbiology
资助者National Natural Science Foundation of China (NSFC) ; National Science and Technology Major Projects for Major New Drugs Innovation and Development of China ; NSFC ; Natural Science Foundation of Heilongjiang Province of China ; 985 Project grant of Peking University Health Science Center ; National Natural Science Foundation of China (NSFC) ; National Science and Technology Major Projects for Major New Drugs Innovation and Development of China ; NSFC ; Natural Science Foundation of Heilongjiang Province of China ; 985 Project grant of Peking University Health Science Center
研究领域[WOS]Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]HUMAN INTESTINAL BACTERIA ; BIOLOGICAL NUTRIENT REMOVAL ; WASTE-WATER TREATMENT ; ANTIOXIDANT ACTIVITY ; MAMMALIAN LIGNANS ; SLUDGE-DIGESTION ; NITROGEN REMOVAL ; CONTROL STRATEGY ; DIGLUCOSIDE ; PHYTOESTROGENS
英文摘要

Aims Secoisolariciresinol (SECO) is increasingly recognized for potential clinical application because of its preventive effects against breast and colon cancers, atherosclerosis and diabetes, and its production through biotransformation has been attempted. However, previously reported bacteria all required stringent anaerobic culture conditions, precluding large-scale production. Here, we report the isolation and characterization of bacteria that produce SECO under less stringent anaerobic culture conditions. Methods and Results Using defatted flaxseed as raw material, we isolated a facultative anaerobic bacterium from human faeces that hydrolysed secoisolariciresinol diglucoside-3-hydroxy-3-methyl glutaric acid (SDG-HMGA) oligomers in flaxseed to produce SECO. Both conventional assays and 16S rRNA gene sequence analysis demonstrated its close relatedness with Bacteroides uniformis. The transformation efficiency of SDG in defatted flaxseed to SECO was more than 80% by this bacterial strain. We investigated factors that might influence fermentation, such as redox potential and pH, for large-scale fermentation of defatted flaxseed to produce SECO. Conclusions The method to produce SECO through biotransformation of defatted flaxseed with this bacterial strain is highly efficient and economic. Significance and Impact of the Study This bacterial strain can transform SDG to SECO under less stringent anaerobic culture conditions, which will greatly facilitate industry-scale production of SECO.

语种英语
所属项目编号30672622 ; 2011ZX09102-011-06 ; 30970078 ; 30370774 ; 30870098 ; 30970119
资助者National Natural Science Foundation of China (NSFC) ; National Science and Technology Major Projects for Major New Drugs Innovation and Development of China ; NSFC ; Natural Science Foundation of Heilongjiang Province of China ; 985 Project grant of Peking University Health Science Center ; National Natural Science Foundation of China (NSFC) ; National Science and Technology Major Projects for Major New Drugs Innovation and Development of China ; NSFC ; Natural Science Foundation of Heilongjiang Province of China ; 985 Project grant of Peking University Health Science Center
WOS记录号WOS:000311292400006
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51453
Collection北京大学药学院
作者单位1.Peking Univ, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
4.Harbin Med Univ, Genom Res Ctr, Harbin 150081, Peoples R China
Recommended Citation
GB/T 7714
Li, M. -X.,Zhu, H. -Y.,Yang, D. -H.,et al. Production of secoisolariciresinol from defatted flaxseed by bacterial biotransformation[J]. JOURNAL OF APPLIED MICROBIOLOGY,2012,113(6):1352-1361.
APA Li, M. -X..,Zhu, H. -Y..,Yang, D. -H..,Ma, X. -Q..,Wang, C. -Z..,...&Liu, S. -L..(2012).Production of secoisolariciresinol from defatted flaxseed by bacterial biotransformation.JOURNAL OF APPLIED MICROBIOLOGY,113(6),1352-1361.
MLA Li, M. -X.,et al."Production of secoisolariciresinol from defatted flaxseed by bacterial biotransformation".JOURNAL OF APPLIED MICROBIOLOGY 113.6(2012):1352-1361.
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