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IR@PKUHSC  > 北京大学第二临床医学院  > 血液科  > 期刊论文
学科主题: 临床医学
题名:
Recruitment of CD8(+) T cells into bone marrow might explain the suppression of megakaryocyte apoptosis through high expression of CX3CR1(+) in prolonged isolated thrombocytopenia after allogeneic hematopoietic stem cell transplantation
作者: Zhang, Xiao-Hui; Wang, Guo-Xiang; Zhu, Hong-Hu; Liu, Yan-Rong; Xu, Lan-Ping; Han, Wei; Chen, Huan; Chen, Yu-Hong; Wang, Feng-Rong; Wang, Jing-Zhi; Wang, Yu; Zhao, Ting; Chen, Yao; Feng, Ru; Fu, Hai-Xia; Wang, Min; Zhou, Yi; Lv, Meng; Liu, Kai-Yan; Huang, Xiao-Jun
关键词: Thrombocytopenia ; Stem cell transplantation ; CD8(+) T cell ; CX3CR1(+) ; Megakaryocyte
刊名: ANNALS OF HEMATOLOGY
发表日期: 2015-10-01
DOI: 10.1007/s00277-015-2436-6
卷: 94, 期:10, 页:1689-1698
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology
研究领域[WOS]: Hematology
关键词[WOS]: VERSUS-HOST-DISEASE ; PLATELET RECOVERY ; FRACTALKINE ; IMPACT ; IDENTIFICATION ; ASSOCIATION ; INVOLVEMENT ; ENGRAFTMENT ; RELEASE ; PURPURA
英文摘要:

Prolonged isolated thrombocytopenia is a common complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT), which is associated with a poor prognosis. This study aimed to investigate the pathogenesis of prolonged isolated thrombocytopenia (PT). We analysed the expression of CX3CR1 on CD4 and CD8 T cells in bone marrow (BM) and peripheral blood (PB) at +90 days from allo-HSCT recipients with or without PT by flow cytometry analyses. We then determined the megakaryocytes ploidy distributions, apoptosis rate and Fas expression of recipients with or without PT in vitro directly or after depleting CD8(+) T cells or adding purified autologous CD8(+) T cells to CD8(+) T-dep MNCs. We found that the percentage of CD8(+) T cells in BM was higher in the patients with PT than in the controls. The elevated expression of the CX3CR1 was associated with PT. There was a marked increase in the percentage of low ploidy megakaryocytes in the recipients with PT. The depletion of CD8(+) T cells increased the apoptosis of megakaryocytes and decreased the expression of Fas, which could be corrected by re-adding purified autologous CD8(+) T cells. The increase of CD8(+) T cells and CD8(+)/CX3CR1(+) T cells in BM at +90 days were independent risk factors for PT according to multivariate analysis. Our data implied that the recruitment of CD8(+) T cells into BM might explain the suppression of megakaryocyte apoptosis through the elevated expression of CX3CR1(+) in PT after allo-HSCT. CX3CR1 might be a novel treatment target in recipients with PT.

语种: 英语
所属项目编号: 2012BAI38B03 ; 81270643 ; 81470343 ; BMU20140389 ; 7132194 ; 20120001110026
项目资助者: National Key Technology Support Program ; National Natural Science Foundation of China ; Seeding Grant for Medicine and Engineering Sciences of Peking University ; Beijing Natural Science Foundation ; Specialized Research Fund for the Doctoral Program of Higher Education
WOS记录号: WOS:000360665000008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51461
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: Peking Univ, Peking Univ Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China

Recommended Citation:
Zhang, Xiao-Hui,Wang, Guo-Xiang,Zhu, Hong-Hu,et al. Recruitment of CD8(+) T cells into bone marrow might explain the suppression of megakaryocyte apoptosis through high expression of CX3CR1(+) in prolonged isolated thrombocytopenia after allogeneic hematopoietic stem cell transplantation[J]. ANNALS OF HEMATOLOGY,2015,94(10):1689-1698.
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