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Recruitment of CD8(+) T cells into bone marrow might explain the suppression of megakaryocyte apoptosis through high expression of CX3CR1(+) in prolonged isolated thrombocytopenia after allogeneic hematopoietic stem cell transplantation
Zhang, Xiao-Hui; Wang, Guo-Xiang; Zhu, Hong-Hu; Liu, Yan-Rong; Xu, Lan-Ping; Han, Wei; Chen, Huan; Chen, Yu-Hong; Wang, Feng-Rong; Wang, Jing-Zhi; Wang, Yu; Zhao, Ting; Chen, Yao; Feng, Ru; Fu, Hai-Xia; Wang, Min; Zhou, Yi; Lv, Meng; Liu, Kai-Yan; Huang, Xiao-Jun
关键词Thrombocytopenia Stem Cell Transplantation Cd8(+) t Cell Cx3cr1(+) Megakaryocyte
刊名ANNALS OF HEMATOLOGY
2015-10-01
DOI10.1007/s00277-015-2436-6
94期:10页:1689-1698
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Hematology
研究领域[WOS]Hematology
关键词[WOS]VERSUS-HOST-DISEASE ; PLATELET RECOVERY ; FRACTALKINE ; IMPACT ; IDENTIFICATION ; ASSOCIATION ; INVOLVEMENT ; ENGRAFTMENT ; RELEASE ; PURPURA
英文摘要

Prolonged isolated thrombocytopenia is a common complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT), which is associated with a poor prognosis. This study aimed to investigate the pathogenesis of prolonged isolated thrombocytopenia (PT). We analysed the expression of CX3CR1 on CD4 and CD8 T cells in bone marrow (BM) and peripheral blood (PB) at +90 days from allo-HSCT recipients with or without PT by flow cytometry analyses. We then determined the megakaryocytes ploidy distributions, apoptosis rate and Fas expression of recipients with or without PT in vitro directly or after depleting CD8(+) T cells or adding purified autologous CD8(+) T cells to CD8(+) T-dep MNCs. We found that the percentage of CD8(+) T cells in BM was higher in the patients with PT than in the controls. The elevated expression of the CX3CR1 was associated with PT. There was a marked increase in the percentage of low ploidy megakaryocytes in the recipients with PT. The depletion of CD8(+) T cells increased the apoptosis of megakaryocytes and decreased the expression of Fas, which could be corrected by re-adding purified autologous CD8(+) T cells. The increase of CD8(+) T cells and CD8(+)/CX3CR1(+) T cells in BM at +90 days were independent risk factors for PT according to multivariate analysis. Our data implied that the recruitment of CD8(+) T cells into BM might explain the suppression of megakaryocyte apoptosis through the elevated expression of CX3CR1(+) in PT after allo-HSCT. CX3CR1 might be a novel treatment target in recipients with PT.

语种英语
WOS记录号WOS:000360665000008
项目编号2012BAI38B03 ; 81270643 ; 81470343 ; BMU20140389 ; 7132194 ; 20120001110026
资助机构National Key Technology Support Program ; National Natural Science Foundation of China ; Seeding Grant for Medicine and Engineering Sciences of Peking University ; Beijing Natural Science Foundation ; Specialized Research Fund for the Doctoral Program of Higher Education
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51461
专题北京大学第二临床医学院_血液科
医学人文研究院/公共教学部_哲学与社会科学系
作者单位Peking Univ, Peking Univ Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China
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Zhang, Xiao-Hui,Wang, Guo-Xiang,Zhu, Hong-Hu,et al. Recruitment of CD8(+) T cells into bone marrow might explain the suppression of megakaryocyte apoptosis through high expression of CX3CR1(+) in prolonged isolated thrombocytopenia after allogeneic hematopoietic stem cell transplantation[J]. ANNALS OF HEMATOLOGY,2015,94(10):1689-1698.
APA Zhang, Xiao-Hui.,Wang, Guo-Xiang.,Zhu, Hong-Hu.,Liu, Yan-Rong.,Xu, Lan-Ping.,...&Huang, Xiao-Jun.(2015).Recruitment of CD8(+) T cells into bone marrow might explain the suppression of megakaryocyte apoptosis through high expression of CX3CR1(+) in prolonged isolated thrombocytopenia after allogeneic hematopoietic stem cell transplantation.ANNALS OF HEMATOLOGY,94(10),1689-1698.
MLA Zhang, Xiao-Hui,et al."Recruitment of CD8(+) T cells into bone marrow might explain the suppression of megakaryocyte apoptosis through high expression of CX3CR1(+) in prolonged isolated thrombocytopenia after allogeneic hematopoietic stem cell transplantation".ANNALS OF HEMATOLOGY 94.10(2015):1689-1698.
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