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学科主题: 临床医学
题名:
Endogenously generated sulfur dioxide and its vasorelaxant effect in rats
作者: Du, Shu-Xu2; Jin, Hong-Fang2; Bu, Ding-Fang3; Zhao, Xia2; Geng, Bin1; Tang, Chao-Shu1,4; Du, Jun-Bao2,4
关键词: calcium channel ; L-type ; aspartate aminotransferase ; sulfur dioxide ; vasorelaxant effect
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2008-08-01
DOI: 10.1111/j.1745-7254.2008.00845.x
卷: 29, 期:8, 页:923-930
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: HYDROGEN-SULFIDE ; SERUM SULFITE ; NITRIC-OXIDE ; MITOCHONDRIA ; INHIBITION ; EXPRESSION ; CELLS
英文摘要:

Aim: The present study was designed to explore the endogenous production and localization of the sulfur dioxide (SO(2))/aspartate aminotransferase pathway in vascular tissues of rats and to examine its vasorelaxant effect on isolated aortic rings, as well as the possible mechanisms. Methods: The content of SO(2) in the samples was determined by using high performance liquid chromatography with fluorescence detection. Aspartate aminotransferase activity and its gene expression were measured by an enzymatic method and quantitative RT-PCR, respectively. Aspartate aminotransferase mRNA location in aorta was detected by in situ hybridization. The vasorelaxant effect of SO(2) on isolated aortic rings of the rats was investigated in vitro. L-type calcium channel blocker, nicardipine, and L-type calcium channel agonist, Bay K8644, were used to explore the mechanisms by which SO(2) relaxed the aortic rings. Results: Aorta had the highest SO(2) content among the vascular tissues tested (P < 0.01). The aortic aspartate aminotransferase mRNA located in endothelia and vascular smooth muscle cells beneath the endothelial layer. Furthermore, a physiological dose of the SO(2) derivatives (Na(2)SO(3)/NaHSO(3)) relaxed isolated artery rings slightly, whereas higher doses (1-12 mmol/L) relaxed rings in a concentration-dependent manner. Pretreatment with nicardipine eliminated the vasorelaxant response of the norepinephrine-contracted rings to SO(2) completely. Incubation with nicardipine or SO(2) derivatives successfully prevented vasoconstriction induced by Bay K8644. Conclusion: Endogenous SO(2) and its derivatives have a vasorelaxant function, the mechanisms of which might involve the inhibition of the L-type calcium channel.

语种: 英语
WOS记录号: WOS:000258194600006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51466
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Ctr Lab, Beijing 100034, Peoples R China
2.Minist Educ, Key Lab Mol Cardiovasc Dis, Beijing 100083, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
4.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Du, Shu-Xu,Jin, Hong-Fang,Bu, Ding-Fang,et al. Endogenously generated sulfur dioxide and its vasorelaxant effect in rats[J]. ACTA PHARMACOLOGICA SINICA,2008,29(8):923-930.
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