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学科主题: 口腔医学
题名:
Editing genomic DNA in cancer cells with high genetic variance: Benefit or risk?
作者: Wang, Lin1; Wang, Yixiang2; Guo, Chuanbin1
关键词: genome ; genetic variation ; transfection ; gene transfer techniques
刊名: ONCOLOGY REPORTS
发表日期: 2014-05-01
DOI: 10.3892/or.2014.3067
卷: 31, 期:5, 页:2079-2084
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: INSTABILITY ; METASTASIS ; DELIVERY ; PEPTIDE ; S100P
英文摘要:

The generation of stably-transfected cell lines is a common and very important technology in cancer science. Considerable knowledge in the field of life sciences has been gained through the modification of the genetic code. However, there is a risk in evaluating exogenous gene function through editing genomic DNA in a cancer cell with high genetic variance. In the present study, we showed that genomic DNA status should be considered when evaluating the exogenous gene function in a cancer cell line with high variant genome through stable transfection technology, immunostaining, wound healing assay, Transwell invasion assay, real-time PCR, western blot and karyotyping analysis. Our results showed that the S100P expression level was not related to the migration and invasion abilities in these stably transfected cell lines derived from a human salivary adenoid cystic carcinoma cell line SACC-83. The MMP expression pattern was detected by western blot analysis which matched the biological behaviors in these cells. The genomic analysis showed that SACC-83 presented hypotetraploid karyotyping with high variance. Our data indicated that establishment of stable transgenic cancer cell lines should consider the status of genetic variance in a cancer cell to avoid any biased conclusion.

语种: 英语
所属项目编号: 81271150
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000337947200015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51469
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Wang, Lin,Wang, Yixiang,Guo, Chuanbin. Editing genomic DNA in cancer cells with high genetic variance: Benefit or risk?[J]. ONCOLOGY REPORTS,2014,31(5):2079-2084.
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