IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Editing genomic DNA in cancer cells with high genetic variance: Benefit or risk?
Wang, Lin1; Wang, Yixiang2; Guo, Chuanbin1
关键词Genome Genetic Variation Transfection Gene Transfer Techniques
刊名ONCOLOGY REPORTS
2014-05-01
DOI10.3892/or.2014.3067
31期:5页:2079-2084
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]INSTABILITY ; METASTASIS ; DELIVERY ; PEPTIDE ; S100P
英文摘要

The generation of stably-transfected cell lines is a common and very important technology in cancer science. Considerable knowledge in the field of life sciences has been gained through the modification of the genetic code. However, there is a risk in evaluating exogenous gene function through editing genomic DNA in a cancer cell with high genetic variance. In the present study, we showed that genomic DNA status should be considered when evaluating the exogenous gene function in a cancer cell line with high variant genome through stable transfection technology, immunostaining, wound healing assay, Transwell invasion assay, real-time PCR, western blot and karyotyping analysis. Our results showed that the S100P expression level was not related to the migration and invasion abilities in these stably transfected cell lines derived from a human salivary adenoid cystic carcinoma cell line SACC-83. The MMP expression pattern was detected by western blot analysis which matched the biological behaviors in these cells. The genomic analysis showed that SACC-83 presented hypotetraploid karyotyping with high variance. Our data indicated that establishment of stable transgenic cancer cell lines should consider the status of genetic variance in a cancer cell to avoid any biased conclusion.

语种英语
WOS记录号WOS:000337947200015
项目编号81271150
资助机构National Nature Science Foundation of China
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51469
专题北京大学口腔医学院
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
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GB/T 7714
Wang, Lin,Wang, Yixiang,Guo, Chuanbin. Editing genomic DNA in cancer cells with high genetic variance: Benefit or risk?[J]. ONCOLOGY REPORTS,2014,31(5):2079-2084.
APA Wang, Lin,Wang, Yixiang,&Guo, Chuanbin.(2014).Editing genomic DNA in cancer cells with high genetic variance: Benefit or risk?.ONCOLOGY REPORTS,31(5),2079-2084.
MLA Wang, Lin,et al."Editing genomic DNA in cancer cells with high genetic variance: Benefit or risk?".ONCOLOGY REPORTS 31.5(2014):2079-2084.
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