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学科主题临床医学
Hydrogen sulfide ameliorates vascular calcification induced by vitamin D3 plus nicotine in rats
Wu, SY1; Pan, CS1; Geng, B1; Zhao, J1; Yu, F1; Pang, YZ1; Tang, CS1; Qi, YF1
关键词Hydrogen Sulfide Cystathionine-lyase Vascular Calcification Mrna
刊名ACTA PHARMACOLOGICA SINICA
2006-03-01
DOI10.1111/j.1745-7254.2006.00283.x
27期:3页:299-306
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]SMOOTH-MUSCLE-CELLS ; ARTERIAL CALCIFICATION ; IN-VITRO ; AORTIC CALCIFICATION ; NITRIC-OXIDE ; ADRENOMEDULLIN ; MECHANISMS ; H2S ; OSTEOPOROSIS ; EXPRESSION
英文摘要

Aim: To investigate the role of the endogenous cystathionine gamma-synthase (CSE)/hydrogen sulfide (H2S) pathway in vascular calcification in vivo. Methods: A rat vascular calcification model was established by administration of vitamin D3 plus nicotine (VDN). The amount of CSE and osteopontin (OPN) mRNA was determined by using semi-quantitative reverse-transcription polymerase chain reaction. The calcium content, Ca-45(2+) accumulation and alkaline phosphatase (ALP) activity were measured. H2S production and CSE activity were measured. Results: von Kossa staining produced strong positive black/brown staining in areas among the elastic fibers of the medial layer in the calcified aorta. The calcium content, Ca-45(2+) accumulation and ALP activity in calcified arteries increased by 6.77-, 1.42-, and 1.87-fold, respectively, compared with controls. The expression of the OPN gene was upregulated (P < 0.01). Expression of the CSE gene was downregulated. However, calcium content, Ca-45(2+) uptake and ALP activity in the VDN plus NaHS group was lower than that in the VDN group. The content of calcium and Ca-45(2+) accumulation and activity of ALP in the aorta were 34.8%, 40.75% and 63.5% lower in the low-dosage NaHS group than in the VDN group, respectively (P < 0.01), and the calcium content and deposition of Ca-45(2+) and activity of ALP was 83.9%, 37.8 % and 46.2% lower in the aorta in the high-dosage NaHS group than in the VDN group, respectively (P < 0.01). The expression of the OPN gene was downregulated. Conclusion: The production of H2S, and CSE activity were decreased and CSE gene expression was downregulated in rats with vascular calcification. H2S can ameliorate vascular calcification, suggesting that the H2S/CSE pathway plays a regulatory role in the pathogenesis of vascular calcification.

语种英语
WOS记录号WOS:000235587300006
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51479
专题北京大学第一临床医学院_心血管内科
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
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Wu, SY,Pan, CS,Geng, B,et al. Hydrogen sulfide ameliorates vascular calcification induced by vitamin D3 plus nicotine in rats[J]. ACTA PHARMACOLOGICA SINICA,2006,27(3):299-306.
APA Wu, SY.,Pan, CS.,Geng, B.,Zhao, J.,Yu, F.,...&Qi, YF.(2006).Hydrogen sulfide ameliorates vascular calcification induced by vitamin D3 plus nicotine in rats.ACTA PHARMACOLOGICA SINICA,27(3),299-306.
MLA Wu, SY,et al."Hydrogen sulfide ameliorates vascular calcification induced by vitamin D3 plus nicotine in rats".ACTA PHARMACOLOGICA SINICA 27.3(2006):299-306.
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