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Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum
Li Da-Hui1; Jian Gui-Liang1; Zhang Ying-Tao1; Ai Tie-Min1
关键词Defensin Expression Fusarium Oxysporum Rt-pcr Trichosanthes Kirilowii
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
2007-02-01
DOI10.1007/s00253-006-0631-z
74期:1页:146-151
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]ANTIMICROBIAL PEPTIDES ; PLANT DEFENSINS ; HOST-DEFENSE ; GENE FAMILY ; PROTEINS ; PEPPER ; POTATO
英文摘要

The gene encoding Trichosanthes kirilowii defensin (TDEF1) was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR). The newly discovered TDEF1 cDNA contains 231 bp (Genbank accession number DQ526373) and encodes a 76-amino acid protein, which consists of a 29-amino acid signal peptide and a 47-amino acid mature peptide. The partial cDNA, corresponding to the mature peptide coding region of TDEF1, was inserted into bacterial expression vector pET32a(+). Subsequent expression showed that TDEF1 was produced as a 26-kDa fusion protein in the form of inclusion body in Escherichia coli BL21 (DE3). After protein refolding and purification, the fusion TDEF1 displayed an inhibitive activity against the fungal pathogen, Fusarium oxysporum, with EC50 of 247 mu g/ml by means of fungal growth inhibition method.

语种英语
WOS记录号WOS:000243802200018
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51482
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100094, Peoples R China
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Li Da-Hui,Jian Gui-Liang,Zhang Ying-Tao,et al. Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,74(1):146-151.
APA Li Da-Hui,Jian Gui-Liang,Zhang Ying-Tao,&Ai Tie-Min.(2007).Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,74(1),146-151.
MLA Li Da-Hui,et al."Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 74.1(2007):146-151.
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