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学科主题: 药学
题名:
Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum
作者: Li Da-Hui1; Jian Gui-Liang1; Zhang Ying-Tao1; Ai Tie-Min1
关键词: defensin ; expression ; Fusarium oxysporum ; RT-PCR ; Trichosanthes kirilowii
刊名: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
发表日期: 2007-02-01
DOI: 10.1007/s00253-006-0631-z
卷: 74, 期:1, 页:146-151
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
关键词[WOS]: ANTIMICROBIAL PEPTIDES ; PLANT DEFENSINS ; HOST-DEFENSE ; GENE FAMILY ; PROTEINS ; PEPPER ; POTATO
英文摘要:

The gene encoding Trichosanthes kirilowii defensin (TDEF1) was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR). The newly discovered TDEF1 cDNA contains 231 bp (Genbank accession number DQ526373) and encodes a 76-amino acid protein, which consists of a 29-amino acid signal peptide and a 47-amino acid mature peptide. The partial cDNA, corresponding to the mature peptide coding region of TDEF1, was inserted into bacterial expression vector pET32a(+). Subsequent expression showed that TDEF1 was produced as a 26-kDa fusion protein in the form of inclusion body in Escherichia coli BL21 (DE3). After protein refolding and purification, the fusion TDEF1 displayed an inhibitive activity against the fungal pathogen, Fusarium oxysporum, with EC50 of 247 mu g/ml by means of fungal growth inhibition method.

语种: 英语
WOS记录号: WOS:000243802200018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51482
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100094, Peoples R China

Recommended Citation:
Li Da-Hui,Jian Gui-Liang,Zhang Ying-Tao,et al. Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,74(1):146-151.
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