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学科主题基础医学
Molecular cloning and characterization of rat chemokine-like factor 1 and 2
Lou, YX1; Xia, DL1; Han, WL1; Wang, Y1; Li, XT1; Li, Y1; Rui, M1; Ding, PG1; Song, QS1; Zhang, YM1; Ma, DL1
关键词chemokine chemokine-like factor expressed sequence tag assembly linear amplification of cDNA ends
刊名GENE
2003-03-27
DOI10.1016/S0378-1119(03)00450-5
307期:0页:125-132
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Genetics & Heredity
研究领域[WOS]Genetics & Heredity
关键词[WOS]SIGNAL PEPTIDES ; MESSENGER-RNAS ; CHEMOKINE ; CELLS ; IDENTIFICATION ; LYMPHOCYTES ; PREDICTION ; LIGAND ; SITES ; CXC
英文摘要

Chemokine-like factor I (CKLF1) is a newly cloned cytokine with three RNA splicing isoforms. It has chemotactic activities on leukocytes and plays an important role in skeletal muscle regeneration. Here we have isolated two rat homologues of human chemokine-like factors by expressed sequence tag assembly, which are designated as rat chemokine-like factor I and 2 (rat CKLF1, CKLF2). The full-length cDNAs of rat CKLF1 and -2 contain 523 and 682 nucleotides and the open reading frames encoding 98 and 151 amino acids, respectively. Rat CKLF1 and -2 share about 54.1 and 59.6% homologies with human CKLF1 and -2 at the amino acid level; both rat CKLF1 and -2 contain a CX3C motif at their C-terminal regions while human CKLF′s have a CC motif at the same regions. Rat CKLFs are highly expressed in testis, while human CKLFs have a broad expression spectrum across multiple tissues. Recombinant rat CKLF1 can be secreted into the cell culture supernatants and has chemotactic effects on neutrophils, macrophages and lymphocytes, which is similar to human CKLF1, while recombinant rat CKLF2 has weaker chemotactic effects on these cells. These findings show that rat CKLFs have similar bioactivity with human CKLFs, although they are different in tissue distribution and contain different characteristic motifs. (C) 2003 Published by Elsevier Science B.V.

语种英语
WOS记录号WOS:000182793100012
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51488
Collection北京大学基础医学院_免疫学系
作者单位1.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Lou, YX,Xia, DL,Han, WL,et al. Molecular cloning and characterization of rat chemokine-like factor 1 and 2[J]. GENE,2003,307(0):125-132.
APA Lou, YX.,Xia, DL.,Han, WL.,Wang, Y.,Li, XT.,...&Ma, DL.(2003).Molecular cloning and characterization of rat chemokine-like factor 1 and 2.GENE,307(0),125-132.
MLA Lou, YX,et al."Molecular cloning and characterization of rat chemokine-like factor 1 and 2".GENE 307.0(2003):125-132.
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