|Relationship of CD44(+)CD24(-/low) breast cancer stem cells and axillary lymph node metastasis|
|Wei, Wei1; Hu, Hui1; Tan, Haosheng1; Chow, Louis W. C.2; Yip, Adrian Y. S.2; Loo, Wings T. Y.2|
|刊名||JOURNAL OF TRANSLATIONAL MEDICINE|
|收录类别||SCI ; ISTP|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, Research & Experimental|
|研究领域[WOS]||Research & Experimental Medicine|
|关键词[WOS]||MT-100-TC MAMMARY-CARCINOMA ; RATS BEARING ; CD44(+)/CD24(-) ; THERAPY ; MARKERS ; TUMORS|
Background: Axillary node staging plays an important role in the prognostic evaluation and planning of adjuvant treatment. Breast cancer stem cells, identified on the basis of CD44(+)CD24(-/low) expression, are associated with metastases and drug resistance. It is therefore important to investigate the proportion of CD44(+)CD24(-/low) breast cancer stem cells for the diagnosis of metastases in axillary nodes.
Methods: Thirty-two ipsilateral axillary lymph nodes were collected from patients diagnosed with invasive breast cancer. Each lymph node (LN) was divided into two equals - one was examined by H&E staining, while the other was made into a single cell suspension to study the content of CD44(+)CD24(-/low) cells by flow cytometry (FCM). The relationship was investigated between the content of CD44(+)CD24(-/low) cells and metastases in axillary nodes which were confirmed by histology. Associations were tested using the chi-square test (linear-by-linear association), and the significance level was set at a value of p < 0.05.
Results: In the 32 axillary nodes, the level of CD44(+)CD24(-/low) cells was determined to be between 0 and 18.4%: there was no presence of CD44+CD24-/low cells in 9 LNs, of which 2 had confirmed metastasis; there were less than 10% CD44+CD24-/low cells in 12 LNs, of which 6 had confirmed metastasis; and there were more than 10% CD44+CD24-/low cells in 11 LNs, of which 9 had confirmed metastasis. A higher percentage of detected CD44(+)CD24(-/low) cells was significantly associated with more confirmed LN metastases (p = 0.009).
Conclusions: CD44(+)CD24(-/low) breast cancer stem cells might help clinicians to determine the presence of LN metastases. However, its prognostic value remains unclear, while histological diagnosis is still the gold standard.
|作者单位||1.Peking Univ, Shenzhen Hosp, Dept Breast Surg, Shenzhen, Guangdong, Peoples R China|
2.Organisat Oncol & Translat Res & UNIMED Med Inst, Hong Kong, Hong Kong, Peoples R China
|Wei, Wei,Hu, Hui,Tan, Haosheng,et al. Relationship of CD44(+)CD24(-/low) breast cancer stem cells and axillary lymph node metastasis[J]. JOURNAL OF TRANSLATIONAL MEDICINE,2012,suppl.1.|
|APA||Wei, Wei,Hu, Hui,Tan, Haosheng,Chow, Louis W. C.,Yip, Adrian Y. S.,&Loo, Wings T. Y..(2012).Relationship of CD44(+)CD24(-/low) breast cancer stem cells and axillary lymph node metastasis.JOURNAL OF TRANSLATIONAL MEDICINE,suppl.1.|
|MLA||Wei, Wei,et al."Relationship of CD44(+)CD24(-/low) breast cancer stem cells and axillary lymph node metastasis".JOURNAL OF TRANSLATIONAL MEDICINE suppl.1(2012).|