|Effects of Chinese medicine Tongxinluo on hyperglycemia and beta-cell damage in streptozotocin-induced diabetic rats|
|Wang Huai-qing1,3; Zou Jun-jie2; Zhou Xiang-hai1; Ji Li-nong1; Liu Zhi-min2|
|关键词||Tongxinluo Streptozotocin Diabetes Oxidative Stress Antioxidants Beta-cell Function|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||RANDOMIZED CONTROLLED-TRIAL ; OXIDATIVE STRESS ; CARDIOVASCULAR-DISEASE ; REACTIVE OXYGEN ; MELATONIN ; MECHANISM ; INJURY ; OXIDE ; COMPLICATIONS ; INTERVENTION|
Background Oxidative stress has been implicated in the onset and progression of diabetes. Tongxinluo is a traditional Chinese medicine with potent antioxidant properties. The aim of this study was to test the hypothesis that pretreatment with Tongxinluo has similar effects as melatonin on preventing hyperglycemia and beta-cell damage in a rat model of streptozotocin (STZ)-induced diabetes.
Methods Forty male Sprague Dawley rats were randomly assigned to four groups (n=10 each): normal control (NC) group; STZ group (70 mg/kg, i.p.); Tongxinluo (1.0 g.kg(-1).d(-1)) pretreated (TXL+STZ) group and melatonin (200 mu g.kg(-1).d(-1)) pretreated (MLT+STZ) group. Tongxinluo and melatonin were administered by gavage beginning 8 days before STZ injection and continuing until the end of the study (15 days after STZ administration). Blood glucose levels and body weights, malondialdehyde (MDA), and reduced glutathione (GSH) levels were measured, and immunofluorescence studies were performed in all of the groups.
Results Pretreatment with Tongxinluo, as with melatonin, attenuated severe hyperglycemia and weight loss induced by STZ. In pancreatic homogenates, MDA levels were significantly lower and GSH levels were significantly higher in Tongxinluo pretreated group and in melatonin pretreated group than those in STZ group. Values of insulin staining were significantly improved in Tongxinluo pretreated group and in melatonin pretreated group as compared with those in STZ group.
Conclusions Tongxinluo, as melatonin, prevented hyperglycemia and beta-cell destruction induced by STZ in rats through reducing oxidative stress in pancreatic tissues. Tongxinluo may provide an alternative therapy for the prevention and treatment of diabetes. Chin Med J 2012;125(20):3675-3680
|项目编号||2005 CB 523304|
|资助机构||National Basic Research Program (973 Program) in China (Ministry of Science and Technology)|
|作者单位||1.Peking Univ, Dept Endocrinol & Metab, Peoples Hosp, Ctr Diabet, Beijing 100044, Peoples R China|
2.Secondary Mil Med Univ, Dept Endocrinol & Metab, Changzheng Hosp, Shanghai 200003, Peoples R China
3.China Three Gorges Univ, Dept Endocrinol & Metab, Peoples Hosp Yichang 1, Yichang 443000, Hubei, Peoples R China
|Wang Huai-qing,Zou Jun-jie,Zhou Xiang-hai,et al. Effects of Chinese medicine Tongxinluo on hyperglycemia and beta-cell damage in streptozotocin-induced diabetic rats[J]. CHINESE MEDICAL JOURNAL,2012,125(20):3675-3680.|
|APA||Wang Huai-qing,Zou Jun-jie,Zhou Xiang-hai,Ji Li-nong,&Liu Zhi-min.(2012).Effects of Chinese medicine Tongxinluo on hyperglycemia and beta-cell damage in streptozotocin-induced diabetic rats.CHINESE MEDICAL JOURNAL,125(20),3675-3680.|
|MLA||Wang Huai-qing,et al."Effects of Chinese medicine Tongxinluo on hyperglycemia and beta-cell damage in streptozotocin-induced diabetic rats".CHINESE MEDICAL JOURNAL 125.20(2012):3675-3680.|